Evolutionary diversification of epidermal barrier genes in amphibians
Abstract The epidermal differentiation complex (EDC) is a cluster of genes encoding components of the skin barrier in terrestrial vertebrates. EDC genes can be categorized as S100 fused-type protein (SFTP) genes such as filaggrin, which contain two coding exons, and single-coding-exon EDC (SEDC) gen...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18053-7 |
| _version_ | 1818084424020918272 |
|---|---|
| author | Attila Placido Sachslehner Leopold Eckhart |
| author_facet | Attila Placido Sachslehner Leopold Eckhart |
| author_sort | Attila Placido Sachslehner |
| collection | DOAJ |
| description | Abstract The epidermal differentiation complex (EDC) is a cluster of genes encoding components of the skin barrier in terrestrial vertebrates. EDC genes can be categorized as S100 fused-type protein (SFTP) genes such as filaggrin, which contain two coding exons, and single-coding-exon EDC (SEDC) genes such as loricrin. SFTPs are known to be present in amniotes (mammals, reptiles and birds) and amphibians, whereas SEDCs have not yet been reported in amphibians. Here, we show that caecilians (Amphibia: Gymnophiona) have both SFTP and SEDC genes. Two to four SEDC genes were identified in the genomes of Rhinatrema bivittatum, Microcaecilia unicolor and Geotrypetes seraphini. Comparative analysis of tissue transcriptomes indicated predominant expression of SEDC genes in the skin of caecilians. The proteins encoded by caecilian SEDC genes resemble human SEDC proteins, such as involucrin and small proline-rich proteins, with regard to low sequence complexity and high contents of proline, glutamine and lysine. Our data reveal diversification of EDC genes in amphibians and suggest that SEDC-type skin barrier genes have originated either in a common ancestor of tetrapods followed by loss in Batrachia (frogs and salamanders) or, by convergent evolution, in caecilians and amniotes. |
| first_indexed | 2024-12-10T19:53:40Z |
| format | Article |
| id | doaj.art-04914df8726c435bb6c5e3acae252f27 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T19:53:40Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-04914df8726c435bb6c5e3acae252f272022-12-22T01:35:42ZengNature PortfolioScientific Reports2045-23222022-08-011211910.1038/s41598-022-18053-7Evolutionary diversification of epidermal barrier genes in amphibiansAttila Placido Sachslehner0Leopold Eckhart1Skin Biology Laboratory, Department of Dermatology, Medical University of ViennaSkin Biology Laboratory, Department of Dermatology, Medical University of ViennaAbstract The epidermal differentiation complex (EDC) is a cluster of genes encoding components of the skin barrier in terrestrial vertebrates. EDC genes can be categorized as S100 fused-type protein (SFTP) genes such as filaggrin, which contain two coding exons, and single-coding-exon EDC (SEDC) genes such as loricrin. SFTPs are known to be present in amniotes (mammals, reptiles and birds) and amphibians, whereas SEDCs have not yet been reported in amphibians. Here, we show that caecilians (Amphibia: Gymnophiona) have both SFTP and SEDC genes. Two to four SEDC genes were identified in the genomes of Rhinatrema bivittatum, Microcaecilia unicolor and Geotrypetes seraphini. Comparative analysis of tissue transcriptomes indicated predominant expression of SEDC genes in the skin of caecilians. The proteins encoded by caecilian SEDC genes resemble human SEDC proteins, such as involucrin and small proline-rich proteins, with regard to low sequence complexity and high contents of proline, glutamine and lysine. Our data reveal diversification of EDC genes in amphibians and suggest that SEDC-type skin barrier genes have originated either in a common ancestor of tetrapods followed by loss in Batrachia (frogs and salamanders) or, by convergent evolution, in caecilians and amniotes.https://doi.org/10.1038/s41598-022-18053-7 |
| spellingShingle | Attila Placido Sachslehner Leopold Eckhart Evolutionary diversification of epidermal barrier genes in amphibians Scientific Reports |
| title | Evolutionary diversification of epidermal barrier genes in amphibians |
| title_full | Evolutionary diversification of epidermal barrier genes in amphibians |
| title_fullStr | Evolutionary diversification of epidermal barrier genes in amphibians |
| title_full_unstemmed | Evolutionary diversification of epidermal barrier genes in amphibians |
| title_short | Evolutionary diversification of epidermal barrier genes in amphibians |
| title_sort | evolutionary diversification of epidermal barrier genes in amphibians |
| url | https://doi.org/10.1038/s41598-022-18053-7 |
| work_keys_str_mv | AT attilaplacidosachslehner evolutionarydiversificationofepidermalbarriergenesinamphibians AT leopoldeckhart evolutionarydiversificationofepidermalbarriergenesinamphibians |