Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry
The potential development of a rapid and highly sensitive breast cancer (BC) diagnostic method has been increasingly investigated by many researchers in order to significantly improve the diagnosis of this disease that affects millions of women worldwide. Thus, this investigation aimed to establish...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/10/5/291 |
| _version_ | 1797598272012943360 |
|---|---|
| author | Patrícia Sousa Irene Camacho José S. Câmara Rosa Perestrelo |
| author_facet | Patrícia Sousa Irene Camacho José S. Câmara Rosa Perestrelo |
| author_sort | Patrícia Sousa |
| collection | DOAJ |
| description | The potential development of a rapid and highly sensitive breast cancer (BC) diagnostic method has been increasingly investigated by many researchers in order to significantly improve the diagnosis of this disease that affects millions of women worldwide. Thus, this investigation aimed to establish a potential BC urinary peptidomic pattern using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE) coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a useful approach for BC diagnosis. The results of Lowry’s assay demonstrated that the total protein concentration increased after precipitation and that the healthy control group (HCs, 160 to 318 µg/mL, 142 ± DD µg/mL, on average) presented higher total protein content than the BC patients (140 to 311 µg/mL, 115 ± DD µg/mL, on average). Related to MALDI-TOF MS analysis, the results revealed that four peptide ion biosignatures (<i>m</i>/<i>z</i> 1046.5, 1062.5, 1237.7 and 1727.9) allowed the discrimination between BC patients and HCs. The distinction efficiency and accuracy of BC urine peptides were determined by receiver operating characteristic (ROC) curve analysis that enabled the recognition of some features with great sensitivity (88%) and specificity (98%). Therefore, the obtained data revealed MALDI-TOF MS as a powerful tool to explore peptidomic biosignatures due to its speed, sensitivity, and mass accuracy, which allow the establishment of novel disease biomarkers. |
| first_indexed | 2024-03-11T03:19:01Z |
| format | Article |
| id | doaj.art-049779d9890e4ff6ae9584b5d6cc37a1 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-11T03:19:01Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-049779d9890e4ff6ae9584b5d6cc37a12023-11-18T03:16:00ZengMDPI AGSeparations2297-87392023-05-0110529110.3390/separations10050291Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass SpectrometryPatrícia Sousa0Irene Camacho1José S. Câmara2Rosa Perestrelo3CQM—Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, PortugalFaculdade de Ciências da Vida, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, PortugalCQM—Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, PortugalCQM—Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, PortugalThe potential development of a rapid and highly sensitive breast cancer (BC) diagnostic method has been increasingly investigated by many researchers in order to significantly improve the diagnosis of this disease that affects millions of women worldwide. Thus, this investigation aimed to establish a potential BC urinary peptidomic pattern using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D SDS-PAGE) coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a useful approach for BC diagnosis. The results of Lowry’s assay demonstrated that the total protein concentration increased after precipitation and that the healthy control group (HCs, 160 to 318 µg/mL, 142 ± DD µg/mL, on average) presented higher total protein content than the BC patients (140 to 311 µg/mL, 115 ± DD µg/mL, on average). Related to MALDI-TOF MS analysis, the results revealed that four peptide ion biosignatures (<i>m</i>/<i>z</i> 1046.5, 1062.5, 1237.7 and 1727.9) allowed the discrimination between BC patients and HCs. The distinction efficiency and accuracy of BC urine peptides were determined by receiver operating characteristic (ROC) curve analysis that enabled the recognition of some features with great sensitivity (88%) and specificity (98%). Therefore, the obtained data revealed MALDI-TOF MS as a powerful tool to explore peptidomic biosignatures due to its speed, sensitivity, and mass accuracy, which allow the establishment of novel disease biomarkers.https://www.mdpi.com/2297-8739/10/5/291biomarkersbreast cancer1D SDS-PAGEMALDI-TOF MSurine peptidomic |
| spellingShingle | Patrícia Sousa Irene Camacho José S. Câmara Rosa Perestrelo Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry Separations biomarkers breast cancer 1D SDS-PAGE MALDI-TOF MS urine peptidomic |
| title | Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry |
| title_full | Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry |
| title_fullStr | Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry |
| title_full_unstemmed | Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry |
| title_short | Urinary Proteomic/Peptidomic Biosignature of Breast Cancer Patients Using 1D SDS-PAGE Combined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry |
| title_sort | urinary proteomic peptidomic biosignature of breast cancer patients using 1d sds page combined with matrix assisted laser desorption ionization time of flight mass spectrometry |
| topic | biomarkers breast cancer 1D SDS-PAGE MALDI-TOF MS urine peptidomic |
| url | https://www.mdpi.com/2297-8739/10/5/291 |
| work_keys_str_mv | AT patriciasousa urinaryproteomicpeptidomicbiosignatureofbreastcancerpatientsusing1dsdspagecombinedwithmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT irenecamacho urinaryproteomicpeptidomicbiosignatureofbreastcancerpatientsusing1dsdspagecombinedwithmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT josescamara urinaryproteomicpeptidomicbiosignatureofbreastcancerpatientsusing1dsdspagecombinedwithmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry AT rosaperestrelo urinaryproteomicpeptidomicbiosignatureofbreastcancerpatientsusing1dsdspagecombinedwithmatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry |