Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of Australia
ABSTRACT: Dairy cows can be reservoirs of foodborne pathogens such as Salmonella that pose serious public health risks to humans. The study was designed to examine the molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cow...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S002203022101078X |
| _version_ | 1818114067949158400 |
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| author | J.W. Aleri S. Sahibzada A. Harb A.D. Fisher F.K. Waichigo T. Lee I.D. Robertson S. Abraham |
| author_facet | J.W. Aleri S. Sahibzada A. Harb A.D. Fisher F.K. Waichigo T. Lee I.D. Robertson S. Abraham |
| author_sort | J.W. Aleri |
| collection | DOAJ |
| description | ABSTRACT: Dairy cows can be reservoirs of foodborne pathogens such as Salmonella that pose serious public health risks to humans. The study was designed to examine the molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in the pasture-based system of Australia. A total of 838 animals (328 heifer calves and 510 lactating cows) from 22 farms were sampled. Overall, 54 Salmonella isolates were recovered (calves 28/328 and cows 26/510). A herd-level Salmonella prevalence of 50% (95% confidence interval: 31%–69%) was recorded. Within-herd prevalence for Salmonella ranged between 4%–29% and 4%–45% among the heifer calves and adult lactating cows, respectively. Three different serovars were identified with Salmonella Infantis being the most common serovar (n = 33, 61%) followed by Salmonella Kiambu (n = 20, 37.0%) and one isolate of Salmonella Cerro (2%). The highest antimicrobial resistance prevalence of Salmonella isolates was found against streptomycin (n = 31, 57%), followed by cefoxitin (n = 12, 22%), ceftriaxone (n = 2, 4%), and chloramphenicol (n = 1, 2%). Multiple class resistance was observed on 4 isolates against cefoxitin, chloramphenicol, and streptomycin. Multilocus sequence types ST32 (61%), ST309 (37%), and ST367 (2%) were strongly linked to the serovars Salmonella Infantis, Salmonella Kiambu, and Salmonella Cerro, respectively. Whole genome sequencing of Salmonella isolates detected only 2 resistance genes: aac(6′) gene that confers resistance against aminoglycosides among 40.7% of the isolates, and a single isolate positive for the blaDHA-16 gene. Two distinct clusters among the serovars were observed suggesting 2 independent sources of spread. Despite the low prevalence of antimicrobial resistance among Salmonella from the dairy farms, our findings contribute to the regional and national understanding of antimicrobial resistance in dairy herds in Australia. There is need for continued antimicrobial resistance stewardship and surveillance programs to ensure the production of high-quality food products and the long-term protection of both animal and human health. |
| first_indexed | 2024-12-11T03:44:50Z |
| format | Article |
| id | doaj.art-04987b58c68e48beb6e0adaf152b5cfe |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-12-11T03:44:50Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-04987b58c68e48beb6e0adaf152b5cfe2022-12-22T01:22:03ZengElsevierJournal of Dairy Science0022-03022022-02-01105214931503Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of AustraliaJ.W. Aleri0S. Sahibzada1A. Harb2A.D. Fisher3F.K. Waichigo4T. Lee5I.D. Robertson6S. Abraham7School of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; Centre for Animal Production and Health, Future Foods Institute, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; Antimicrobial Resistance and Infectious Diseases Research Laboratory, Harry Butler Institute, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; Corresponding authorSchool of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; Antimicrobial Resistance and Infectious Diseases Research Laboratory, Harry Butler Institute, Murdoch University, 90 South Street, Murdoch, 6150 WA, AustraliaSchool of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; Antimicrobial Resistance and Infectious Diseases Research Laboratory, Harry Butler Institute, Murdoch University, 90 South Street, Murdoch, 6150 WA, AustraliaFaculty of Veterinary and Agricultural Sciences, University of Melbourne, 250 Princes Highway, Werribee, 3030 VIC, Australia; Animal Welfare Science Centre, University of Melbourne, 3010 VIC, AustraliaBrunswick Veterinary Services, 27 Ommaney Road, Brunswick Junction, 6224 WA, AustraliaSchool of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; Antimicrobial Resistance and Infectious Diseases Research Laboratory, Harry Butler Institute, Murdoch University, 90 South Street, Murdoch, 6150 WA, AustraliaSchool of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; College of Veterinary Medicine, Huazhong Agricultural University. Wuhan, Hubei, 430070, ChinaSchool of Veterinary Medicine, College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, 6150 WA, Australia; Antimicrobial Resistance and Infectious Diseases Research Laboratory, Harry Butler Institute, Murdoch University, 90 South Street, Murdoch, 6150 WA, AustraliaABSTRACT: Dairy cows can be reservoirs of foodborne pathogens such as Salmonella that pose serious public health risks to humans. The study was designed to examine the molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in the pasture-based system of Australia. A total of 838 animals (328 heifer calves and 510 lactating cows) from 22 farms were sampled. Overall, 54 Salmonella isolates were recovered (calves 28/328 and cows 26/510). A herd-level Salmonella prevalence of 50% (95% confidence interval: 31%–69%) was recorded. Within-herd prevalence for Salmonella ranged between 4%–29% and 4%–45% among the heifer calves and adult lactating cows, respectively. Three different serovars were identified with Salmonella Infantis being the most common serovar (n = 33, 61%) followed by Salmonella Kiambu (n = 20, 37.0%) and one isolate of Salmonella Cerro (2%). The highest antimicrobial resistance prevalence of Salmonella isolates was found against streptomycin (n = 31, 57%), followed by cefoxitin (n = 12, 22%), ceftriaxone (n = 2, 4%), and chloramphenicol (n = 1, 2%). Multiple class resistance was observed on 4 isolates against cefoxitin, chloramphenicol, and streptomycin. Multilocus sequence types ST32 (61%), ST309 (37%), and ST367 (2%) were strongly linked to the serovars Salmonella Infantis, Salmonella Kiambu, and Salmonella Cerro, respectively. Whole genome sequencing of Salmonella isolates detected only 2 resistance genes: aac(6′) gene that confers resistance against aminoglycosides among 40.7% of the isolates, and a single isolate positive for the blaDHA-16 gene. Two distinct clusters among the serovars were observed suggesting 2 independent sources of spread. Despite the low prevalence of antimicrobial resistance among Salmonella from the dairy farms, our findings contribute to the regional and national understanding of antimicrobial resistance in dairy herds in Australia. There is need for continued antimicrobial resistance stewardship and surveillance programs to ensure the production of high-quality food products and the long-term protection of both animal and human health.http://www.sciencedirect.com/science/article/pii/S002203022101078XprevalenceSalmonellaSalmonella Infantiszoonoses |
| spellingShingle | J.W. Aleri S. Sahibzada A. Harb A.D. Fisher F.K. Waichigo T. Lee I.D. Robertson S. Abraham Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of Australia Journal of Dairy Science prevalence Salmonella Salmonella Infantis zoonoses |
| title | Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of Australia |
| title_full | Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of Australia |
| title_fullStr | Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of Australia |
| title_full_unstemmed | Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of Australia |
| title_short | Molecular epidemiology and antimicrobial resistance profiles of Salmonella isolates from dairy heifer calves and adult lactating cows in a Mediterranean pasture-based system of Australia |
| title_sort | molecular epidemiology and antimicrobial resistance profiles of salmonella isolates from dairy heifer calves and adult lactating cows in a mediterranean pasture based system of australia |
| topic | prevalence Salmonella Salmonella Infantis zoonoses |
| url | http://www.sciencedirect.com/science/article/pii/S002203022101078X |
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