Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects
The utilization of papaya plants (Carica papaya L) heretofore is focusing on their aril/mesocarp, leaves, and flowers, while the seeds are still neglected. Thus, this recent study attempts to make use of papaya seeds as a green-synthesis bioreductor of silver nanoparticles. It aims at finding out th...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang
2023-07-01
|
| Series: | Walisongo Journal of Chemistry |
| Subjects: | |
| Online Access: | https://journal.walisongo.ac.id/index.php/wjc/article/view/14660 |
| _version_ | 1827784466294112256 |
|---|---|
| author | Alifa Noora Rahma Suratno Suratno |
| author_facet | Alifa Noora Rahma Suratno Suratno |
| author_sort | Alifa Noora Rahma |
| collection | DOAJ |
| description | The utilization of papaya plants (Carica papaya L) heretofore is focusing on their aril/mesocarp, leaves, and flowers, while the seeds are still neglected. Thus, this recent study attempts to make use of papaya seeds as a green-synthesis bioreductor of silver nanoparticles. It aims at finding out the characteristics of silver nanoparticles of ethanol extract of papaya seeds (AgNPs-EEPS), investigating the optimization as well as the characteristics of the AgNPs-EEPS anti-acne cream formula, and examining the antibacterial activity of Propionibacterium acnes on the AgNPs-EEPS anti-acne cream. The software design expert with factorial design method was applied to optimize the AgNPs-EEPS biosynthesis and cream formula. The analysis of the formation of AgNPs-EEPS characteristics, employing various levels of temperature and extract concentration, and applying UV- VIS spectrophotometry, resulted in a wavelength of 415 nm. Meanwhile, the implementation of particle size distribution testing, using PSD on the optimum concentration by 6% with temperature 65°C, generated a wavelength of 54 nm, pI 0.820, and zeta potential -7,7 mV. The optimization of the AgNPs-EEPS cream formula obtained optimum results at a stearic acid level 7 and cetyl alcohol 6. The characteristics of the cream produced a cream that was physically stable and met the requirements of good topical preparation. The anti-acne cream from silver nanoparticles ethanol extract of papaya seeds has the antibacterial activity of Propionibacterium acnes which belongs to the moderate category. |
| first_indexed | 2024-03-11T16:04:40Z |
| format | Article |
| id | doaj.art-049a7bb2c160419aa392e6dd4353e934 |
| institution | Directory Open Access Journal |
| issn | 2549-385X 2621-5985 |
| language | English |
| last_indexed | 2024-03-11T16:04:40Z |
| publishDate | 2023-07-01 |
| publisher | Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang |
| record_format | Article |
| series | Walisongo Journal of Chemistry |
| spelling | doaj.art-049a7bb2c160419aa392e6dd4353e9342023-10-25T05:15:38ZengDepartment of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Walisongo SemarangWalisongo Journal of Chemistry2549-385X2621-59852023-07-0161122110.21580/wjc.v6i1.146604407Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial EffectsAlifa Noora Rahma0Suratno Suratno1Department of Pharmacy, the Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Central JavaDepartment of Pharmacognosy, Semmelweis University, BudapestThe utilization of papaya plants (Carica papaya L) heretofore is focusing on their aril/mesocarp, leaves, and flowers, while the seeds are still neglected. Thus, this recent study attempts to make use of papaya seeds as a green-synthesis bioreductor of silver nanoparticles. It aims at finding out the characteristics of silver nanoparticles of ethanol extract of papaya seeds (AgNPs-EEPS), investigating the optimization as well as the characteristics of the AgNPs-EEPS anti-acne cream formula, and examining the antibacterial activity of Propionibacterium acnes on the AgNPs-EEPS anti-acne cream. The software design expert with factorial design method was applied to optimize the AgNPs-EEPS biosynthesis and cream formula. The analysis of the formation of AgNPs-EEPS characteristics, employing various levels of temperature and extract concentration, and applying UV- VIS spectrophotometry, resulted in a wavelength of 415 nm. Meanwhile, the implementation of particle size distribution testing, using PSD on the optimum concentration by 6% with temperature 65°C, generated a wavelength of 54 nm, pI 0.820, and zeta potential -7,7 mV. The optimization of the AgNPs-EEPS cream formula obtained optimum results at a stearic acid level 7 and cetyl alcohol 6. The characteristics of the cream produced a cream that was physically stable and met the requirements of good topical preparation. The anti-acne cream from silver nanoparticles ethanol extract of papaya seeds has the antibacterial activity of Propionibacterium acnes which belongs to the moderate category.https://journal.walisongo.ac.id/index.php/wjc/article/view/14660optimizationsilver nanoparticlescarica papaya l.propionibacterium acnes |
| spellingShingle | Alifa Noora Rahma Suratno Suratno Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects Walisongo Journal of Chemistry optimization silver nanoparticles carica papaya l. propionibacterium acnes |
| title | Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects |
| title_full | Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects |
| title_fullStr | Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects |
| title_full_unstemmed | Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects |
| title_short | Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects |
| title_sort | optimization of silver nanoparticle biosynthesis with papaya carica papaya l seed ethanol extract and its evaluation on antibacterial effects |
| topic | optimization silver nanoparticles carica papaya l. propionibacterium acnes |
| url | https://journal.walisongo.ac.id/index.php/wjc/article/view/14660 |
| work_keys_str_mv | AT alifanoorarahma optimizationofsilvernanoparticlebiosynthesiswithpapayacaricapapayalseedethanolextractanditsevaluationonantibacterialeffects AT suratnosuratno optimizationofsilvernanoparticlebiosynthesiswithpapayacaricapapayalseedethanolextractanditsevaluationonantibacterialeffects |