Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks
<p>Abstract</p> <p>Background</p> <p>Muscle growth depends on the fusion of proliferate satellite cells to existing myofibers. We reported previously that 0–14 day intermittent feeding led to persistent retardation in myofiber hypertrophy. However, how satellite cells r...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | http://www.microbialcellfactories.com/content/11/1/146 |
| _version_ | 1819121907525484544 |
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| author | Li Yue Yang Xiaojing Ni Yingdong Decuypere Eddy Buyse Johan Everaert Nadia Grossmann Roland Zhao Ruqian |
| author_facet | Li Yue Yang Xiaojing Ni Yingdong Decuypere Eddy Buyse Johan Everaert Nadia Grossmann Roland Zhao Ruqian |
| author_sort | Li Yue |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Muscle growth depends on the fusion of proliferate satellite cells to existing myofibers. We reported previously that 0–14 day intermittent feeding led to persistent retardation in myofiber hypertrophy. However, how satellite cells respond to such nutritional insult has not been adequately elucidated.</p> <p>Results</p> <p>One-day-old broiler chicks were allocated to control (Con, <it>ad libitum</it> feeding), intermittent feeding (IF, feed provided on alternate days) and re-feeding (RF, 2 days <it>ad libitum</it> feeding after 12 days of intermittent feeding) groups. Chickens were killed on Day 15 and satellite cells were isolated. When cultured, satellite cells from the IF group demonstrated significant retardation in proliferation and differentiation potential, while RF partly restored the proliferation rate and differentiation potential of the satellite cells. Significant up-regulation of insulin like growth factor I receptor (IGF-IR) (<it>P<</it>0.05) and thyroid hormone receptor α (TRα) (<it>P<</it>0.05), and down-regulation of growth hormone receptor (GHR) (<it>P<</it>0.01) and IGF-I (<it>P<</it>0.01) mRNA expression was observed in freshly isolated IF satellite cells when compared with Con cells. In RF cells, the mRNA expression of IGF-I was higher (<it>P<</it>0.05) and of TRα was lower (<it>P<</it>0.01) than in IF cells, suggesting that RF restored the mRNA expression of TRα and IGF-I, but not of GHR and IGF-IR. The Bax/Bcl-2 ratio tended to increase in the IF group, which was reversed in the RF group (<it>P<</it>0.05), indicating that RF reduced the pro-apoptotic influence of IF. Moreover, no significant effect of T<sub>3</sub> was detected on cell survival in IF cells compared with Con (<it>P</it><0.001) or RF (<it>P<</it>0.05) cells.</p> <p>Conclusions</p> <p>These data suggest that early-age feed restriction inhibits the proliferation and differentiation of satellite cells, induces changes in mRNA expression of the GH/IGF-I and thyroid hormone receptors in satellite cells, as well as blunted sensitivity of satellite cells to T<sub>3</sub>, and that RF partially reverses these effects. Thus, a moderate nutritional strategy for feed restriction should be chosen in early chick rearing systems.</p> |
| first_indexed | 2024-12-22T06:44:01Z |
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| institution | Directory Open Access Journal |
| issn | 1674-9782 2049-1891 |
| language | English |
| last_indexed | 2024-12-22T06:44:01Z |
| publishDate | 2012-11-01 |
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| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-049b3dfcbaf3438bb0335c075ac5a1f32022-12-21T18:35:21ZengBMCJournal of Animal Science and Biotechnology1674-97822049-18912012-11-01313310.1186/2049-1891-3-33Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicksLi YueYang XiaojingNi YingdongDecuypere EddyBuyse JohanEveraert NadiaGrossmann RolandZhao Ruqian<p>Abstract</p> <p>Background</p> <p>Muscle growth depends on the fusion of proliferate satellite cells to existing myofibers. We reported previously that 0–14 day intermittent feeding led to persistent retardation in myofiber hypertrophy. However, how satellite cells respond to such nutritional insult has not been adequately elucidated.</p> <p>Results</p> <p>One-day-old broiler chicks were allocated to control (Con, <it>ad libitum</it> feeding), intermittent feeding (IF, feed provided on alternate days) and re-feeding (RF, 2 days <it>ad libitum</it> feeding after 12 days of intermittent feeding) groups. Chickens were killed on Day 15 and satellite cells were isolated. When cultured, satellite cells from the IF group demonstrated significant retardation in proliferation and differentiation potential, while RF partly restored the proliferation rate and differentiation potential of the satellite cells. Significant up-regulation of insulin like growth factor I receptor (IGF-IR) (<it>P<</it>0.05) and thyroid hormone receptor α (TRα) (<it>P<</it>0.05), and down-regulation of growth hormone receptor (GHR) (<it>P<</it>0.01) and IGF-I (<it>P<</it>0.01) mRNA expression was observed in freshly isolated IF satellite cells when compared with Con cells. In RF cells, the mRNA expression of IGF-I was higher (<it>P<</it>0.05) and of TRα was lower (<it>P<</it>0.01) than in IF cells, suggesting that RF restored the mRNA expression of TRα and IGF-I, but not of GHR and IGF-IR. The Bax/Bcl-2 ratio tended to increase in the IF group, which was reversed in the RF group (<it>P<</it>0.05), indicating that RF reduced the pro-apoptotic influence of IF. Moreover, no significant effect of T<sub>3</sub> was detected on cell survival in IF cells compared with Con (<it>P</it><0.001) or RF (<it>P<</it>0.05) cells.</p> <p>Conclusions</p> <p>These data suggest that early-age feed restriction inhibits the proliferation and differentiation of satellite cells, induces changes in mRNA expression of the GH/IGF-I and thyroid hormone receptors in satellite cells, as well as blunted sensitivity of satellite cells to T<sub>3</sub>, and that RF partially reverses these effects. Thus, a moderate nutritional strategy for feed restriction should be chosen in early chick rearing systems.</p>http://www.microbialcellfactories.com/content/11/1/146Feed restrictionSatellite cellsProliferationGene expressionChicken |
| spellingShingle | Li Yue Yang Xiaojing Ni Yingdong Decuypere Eddy Buyse Johan Everaert Nadia Grossmann Roland Zhao Ruqian Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks Journal of Animal Science and Biotechnology Feed restriction Satellite cells Proliferation Gene expression Chicken |
| title | Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks |
| title_full | Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks |
| title_fullStr | Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks |
| title_full_unstemmed | Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks |
| title_short | Early-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks |
| title_sort | early age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks |
| topic | Feed restriction Satellite cells Proliferation Gene expression Chicken |
| url | http://www.microbialcellfactories.com/content/11/1/146 |
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