Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
Metal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu<sup>2+</sup> contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu<sup>2+</sup...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/11/1380 |
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| author | Binfeng Yin Xinhua Wan Changcheng Qian A. S. M. Muhtasim Fuad Sohan Teng Zhou Wenkai Yue |
| author_facet | Binfeng Yin Xinhua Wan Changcheng Qian A. S. M. Muhtasim Fuad Sohan Teng Zhou Wenkai Yue |
| author_sort | Binfeng Yin |
| collection | DOAJ |
| description | Metal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu<sup>2+</sup> contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu<sup>2+</sup> in seawater. In this method, Cu<sup>2+</sup> is reduced to Cu<sup>+</sup> to inhibit horseradish peroxidase (HRP) activity, which then results in the color distortion of the reaction solution. The chip provides both naked eye and spectrophotometer modalities. Cu<sup>2+</sup> concentrations have an ideal linear relationship, with absorbance values ranging from 3.91 nM to 256 μM. The proposed enzyme method-based microfluidic chip detects Cu<sup>2+</sup> with a limit of detection (LOD) of 0.87 nM. Other common metal ions do not affect the operation of the chip. The successful detection of Cu<sup>2+</sup> was achieved using three real seawater samples, verifying the ability of the chip in practical applications. Furthermore, the chip realizes the functions of two AND gates in series and has potential practical implementations in biochemical detection and biological computing. |
| first_indexed | 2024-03-10T05:15:23Z |
| format | Article |
| id | doaj.art-049ba2f0a7314564bf66d5cbcf11e943 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T05:15:23Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-049ba2f0a7314564bf66d5cbcf11e9432023-11-23T00:26:40ZengMDPI AGMicromachines2072-666X2021-11-011211138010.3390/mi12111380Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper IonsBinfeng Yin0Xinhua Wan1Changcheng Qian2A. S. M. Muhtasim Fuad Sohan3Teng Zhou4Wenkai Yue5School of Mechanical Engineering, Yangzhou University, Yangzhou 225127, ChinaSchool of Mechanical Engineering, Yangzhou University, Yangzhou 225127, ChinaSchool of Mechanical Engineering, Yangzhou University, Yangzhou 225127, ChinaSchool of Mechanical Engineering, Yangzhou University, Yangzhou 225127, ChinaMechanical and Electrical Engineering College, Hainan University, Haikou 570228, ChinaSchool of Mechanical Engineering, Yangzhou University, Yangzhou 225127, ChinaMetal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu<sup>2+</sup> contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu<sup>2+</sup> in seawater. In this method, Cu<sup>2+</sup> is reduced to Cu<sup>+</sup> to inhibit horseradish peroxidase (HRP) activity, which then results in the color distortion of the reaction solution. The chip provides both naked eye and spectrophotometer modalities. Cu<sup>2+</sup> concentrations have an ideal linear relationship, with absorbance values ranging from 3.91 nM to 256 μM. The proposed enzyme method-based microfluidic chip detects Cu<sup>2+</sup> with a limit of detection (LOD) of 0.87 nM. Other common metal ions do not affect the operation of the chip. The successful detection of Cu<sup>2+</sup> was achieved using three real seawater samples, verifying the ability of the chip in practical applications. Furthermore, the chip realizes the functions of two AND gates in series and has potential practical implementations in biochemical detection and biological computing.https://www.mdpi.com/2072-666X/12/11/1380biological logic gateCu<sup>2+</sup> detectionmicrofluidic chip |
| spellingShingle | Binfeng Yin Xinhua Wan Changcheng Qian A. S. M. Muhtasim Fuad Sohan Teng Zhou Wenkai Yue Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions Micromachines biological logic gate Cu<sup>2+</sup> detection microfluidic chip |
| title | Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions |
| title_full | Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions |
| title_fullStr | Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions |
| title_full_unstemmed | Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions |
| title_short | Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions |
| title_sort | enzyme method based microfluidic chip for the rapid detection of copper ions |
| topic | biological logic gate Cu<sup>2+</sup> detection microfluidic chip |
| url | https://www.mdpi.com/2072-666X/12/11/1380 |
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