Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis
Abstract Bacillus cereus is well known as a causative agent of foodborne gastrointestinal diseases and systemic non-gastrointestinal diseases. We have recently identified a pathogenic B. cereus (named H2) from a deep-sea cold-seep. H2 possesses the pyroptosis-inducing capacity and contains a number...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2022-07-01
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| Series: | Cell Death Discovery |
| Online Access: | https://doi.org/10.1038/s41420-022-01091-5 |
| _version_ | 1828775032868831232 |
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| author | Yan Zhao Li Sun |
| author_facet | Yan Zhao Li Sun |
| author_sort | Yan Zhao |
| collection | DOAJ |
| description | Abstract Bacillus cereus is well known as a causative agent of foodborne gastrointestinal diseases and systemic non-gastrointestinal diseases. We have recently identified a pathogenic B. cereus (named H2) from a deep-sea cold-seep. H2 possesses the pyroptosis-inducing capacity and contains a number of enterotoxins including cytotoxin K (CytK). In the present work, we examined the cytotoxicity of the CytK of H2 to human macrophages. CytK bound macrophages by interaction with the plasma membrane and caused cellular structure damage. CytK−cell interaction triggered rapid pyroptosis mediated by caspase 1-activated gasdermin D (GSDMD). CytK-induced pyroptosis required NLRP3 inflammasome activation, K+ efflux, and intracellular Ca2+ accumulation. CytK exhibited apparent binding to several cytomembrane lipids, in particular phosphatidic acid, which proved to be essential to CytK-elicited cell death. Together, these results add new insights into the cytotoxic mechanism of CytK. |
| first_indexed | 2024-12-11T15:33:02Z |
| format | Article |
| id | doaj.art-049fc047ce9d47dabf8af18136c6d265 |
| institution | Directory Open Access Journal |
| issn | 2058-7716 |
| language | English |
| last_indexed | 2024-12-11T15:33:02Z |
| publishDate | 2022-07-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death Discovery |
| spelling | doaj.art-049fc047ce9d47dabf8af18136c6d2652022-12-22T01:00:01ZengNature Publishing GroupCell Death Discovery2058-77162022-07-01811810.1038/s41420-022-01091-5Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosisYan Zhao0Li Sun1CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, CAS Center for Ocean Mega-Science, Chinese Academy of SciencesCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, CAS Center for Ocean Mega-Science, Chinese Academy of SciencesAbstract Bacillus cereus is well known as a causative agent of foodborne gastrointestinal diseases and systemic non-gastrointestinal diseases. We have recently identified a pathogenic B. cereus (named H2) from a deep-sea cold-seep. H2 possesses the pyroptosis-inducing capacity and contains a number of enterotoxins including cytotoxin K (CytK). In the present work, we examined the cytotoxicity of the CytK of H2 to human macrophages. CytK bound macrophages by interaction with the plasma membrane and caused cellular structure damage. CytK−cell interaction triggered rapid pyroptosis mediated by caspase 1-activated gasdermin D (GSDMD). CytK-induced pyroptosis required NLRP3 inflammasome activation, K+ efflux, and intracellular Ca2+ accumulation. CytK exhibited apparent binding to several cytomembrane lipids, in particular phosphatidic acid, which proved to be essential to CytK-elicited cell death. Together, these results add new insights into the cytotoxic mechanism of CytK.https://doi.org/10.1038/s41420-022-01091-5 |
| spellingShingle | Yan Zhao Li Sun Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis Cell Death Discovery |
| title | Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis |
| title_full | Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis |
| title_fullStr | Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis |
| title_full_unstemmed | Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis |
| title_short | Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis |
| title_sort | bacillus cereus cytotoxin k triggers gasdermin d dependent pyroptosis |
| url | https://doi.org/10.1038/s41420-022-01091-5 |
| work_keys_str_mv | AT yanzhao bacilluscereuscytotoxinktriggersgasderminddependentpyroptosis AT lisun bacilluscereuscytotoxinktriggersgasderminddependentpyroptosis |