CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives
Background: Early detection of pathogen-associated diseases are critical for effective treatment. Rapid, specific, sensitive, and cost-effective diagnostic technologies continue to be challenging to develop. The current gold standard for pathogen detection, polymerase chain reaction technology, has...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Advanced Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123222002405 |
| _version_ | 1797769752540610560 |
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| author | Tao Huang Rui Zhang Jinming Li |
| author_facet | Tao Huang Rui Zhang Jinming Li |
| author_sort | Tao Huang |
| collection | DOAJ |
| description | Background: Early detection of pathogen-associated diseases are critical for effective treatment. Rapid, specific, sensitive, and cost-effective diagnostic technologies continue to be challenging to develop. The current gold standard for pathogen detection, polymerase chain reaction technology, has limitations such as long operational cycles, high cost, and high technician and instrumentation requirements. Aim of review: This review examines and highlights the technical advancements of CRISPR-Cas in pathogen detection and provides an outlook for future development, multi-application scenarios, and clinical translation. Key scientific concepts of review: Approaches enabling clinical detection of pathogen nucleic acids that are highly sensitive, specific, cheap, and portable are necessary. CRISPR-Cas9 specificity in targeting nucleic acids and “collateral cleavage” activity of CRISPR-Cas12/Cas13/Cas14 show significant promise in nucleic acid detection technology. These methods have a high specificity, versatility, and rapid detection cycle. In this paper, CRISPR-Cas-based detection methods are discussed in depth. Although CRISPR-Cas-mediated pathogen diagnostic solutions face challenges, their powerful capabilities will pave the way for ideal diagnostic tools. |
| first_indexed | 2024-03-12T21:13:32Z |
| format | Article |
| id | doaj.art-04a047499d804e81abd7a460d9a81c8c |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-03-12T21:13:32Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-04a047499d804e81abd7a460d9a81c8c2023-07-30T04:22:14ZengElsevierJournal of Advanced Research2090-12322023-08-01506982CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectivesTao Huang0Rui Zhang1Jinming Li2National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, PR China; Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China; Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, PR ChinaCorresponding authors at: National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, NO. 1 Da HuaRoad, DongDan, Beijing 100730, PR China.; National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, PR China; Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China; Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, PR ChinaCorresponding authors at: National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, NO. 1 Da HuaRoad, DongDan, Beijing 100730, PR China.; National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, PR China; Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China; Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, PR ChinaBackground: Early detection of pathogen-associated diseases are critical for effective treatment. Rapid, specific, sensitive, and cost-effective diagnostic technologies continue to be challenging to develop. The current gold standard for pathogen detection, polymerase chain reaction technology, has limitations such as long operational cycles, high cost, and high technician and instrumentation requirements. Aim of review: This review examines and highlights the technical advancements of CRISPR-Cas in pathogen detection and provides an outlook for future development, multi-application scenarios, and clinical translation. Key scientific concepts of review: Approaches enabling clinical detection of pathogen nucleic acids that are highly sensitive, specific, cheap, and portable are necessary. CRISPR-Cas9 specificity in targeting nucleic acids and “collateral cleavage” activity of CRISPR-Cas12/Cas13/Cas14 show significant promise in nucleic acid detection technology. These methods have a high specificity, versatility, and rapid detection cycle. In this paper, CRISPR-Cas-based detection methods are discussed in depth. Although CRISPR-Cas-mediated pathogen diagnostic solutions face challenges, their powerful capabilities will pave the way for ideal diagnostic tools.http://www.sciencedirect.com/science/article/pii/S2090123222002405CRISPR-CasPathogen detectionPoint-of-care testingStandardized testing |
| spellingShingle | Tao Huang Rui Zhang Jinming Li CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives Journal of Advanced Research CRISPR-Cas Pathogen detection Point-of-care testing Standardized testing |
| title | CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives |
| title_full | CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives |
| title_fullStr | CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives |
| title_full_unstemmed | CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives |
| title_short | CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives |
| title_sort | crispr cas based techniques for pathogen detection retrospect recent advances and future perspectives |
| topic | CRISPR-Cas Pathogen detection Point-of-care testing Standardized testing |
| url | http://www.sciencedirect.com/science/article/pii/S2090123222002405 |
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