Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T Cells
<i>FAM49B/Fam49b</i> is a member of the <i>Fam49</i> (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its i...
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MDPI AG
2022-02-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/2/388 |
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| author | Yijian Chen Yuyan Jiang Jihui Lao Yankuan Zhou Lida Su Xiao Huang |
| author_facet | Yijian Chen Yuyan Jiang Jihui Lao Yankuan Zhou Lida Su Xiao Huang |
| author_sort | Yijian Chen |
| collection | DOAJ |
| description | <i>FAM49B/Fam49b</i> is a member of the <i>Fam49</i> (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-driven cytoskeleton remolding. In this study, to further investigate its functions and mechanisms affecting cell behaviors, HEK293T cells (where <i>FAM49B</i> is highly expressed) were used to establish a <i>FAM49B</i> knockout cell line by CRISPR/Cas9 genome editing technology. Our data have clearly revealed that there are triple alleles of <i>FAM49B</i> in the genome of HEK293T cells. Meanwhile, the proliferation deficiency of the <i>FAM49B</i> KO HEK293T cell line and the significantly changed cell proliferation related gene expression profiles, such as CCND1, have been uncovered. At the same time, the existence of isoform 3 has been confirmed in HEK293T cells. Our studies have suggested that <i>FAM49B</i> may also affect cell proliferation via Cyclins, besides its influence on the cytoskeleton. |
| first_indexed | 2024-03-09T21:51:28Z |
| format | Article |
| id | doaj.art-04a0d67195864e3ea0d428dead78a322 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T21:51:28Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-04a0d67195864e3ea0d428dead78a3222023-11-23T20:06:06ZengMDPI AGGenes2073-44252022-02-0113238810.3390/genes13020388Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T CellsYijian Chen0Yuyan Jiang1Jihui Lao2Yankuan Zhou3Lida Su4Xiao Huang5Institute of Cell and Developmental Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Developmental Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Developmental Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Cell and Developmental Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaNeuroscience Care Unit, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, ChinaInstitute of Cell and Developmental Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China<i>FAM49B/Fam49b</i> is a member of the <i>Fam49</i> (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-driven cytoskeleton remolding. In this study, to further investigate its functions and mechanisms affecting cell behaviors, HEK293T cells (where <i>FAM49B</i> is highly expressed) were used to establish a <i>FAM49B</i> knockout cell line by CRISPR/Cas9 genome editing technology. Our data have clearly revealed that there are triple alleles of <i>FAM49B</i> in the genome of HEK293T cells. Meanwhile, the proliferation deficiency of the <i>FAM49B</i> KO HEK293T cell line and the significantly changed cell proliferation related gene expression profiles, such as CCND1, have been uncovered. At the same time, the existence of isoform 3 has been confirmed in HEK293T cells. Our studies have suggested that <i>FAM49B</i> may also affect cell proliferation via Cyclins, besides its influence on the cytoskeleton.https://www.mdpi.com/2073-4425/13/2/388<i>FAM49B</i>/<i>Fam49b</i> (CYRI-B)CCND1cell proliferationCRISPR/Cas9 |
| spellingShingle | Yijian Chen Yuyan Jiang Jihui Lao Yankuan Zhou Lida Su Xiao Huang Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T Cells Genes <i>FAM49B</i>/<i>Fam49b</i> (CYRI-B) CCND1 cell proliferation CRISPR/Cas9 |
| title | Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T Cells |
| title_full | Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T Cells |
| title_fullStr | Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T Cells |
| title_full_unstemmed | Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T Cells |
| title_short | Characterization and Functional Study of <i>FAM49B</i> Reveals Its Effect on Cell Proliferation in HEK293T Cells |
| title_sort | characterization and functional study of i fam49b i reveals its effect on cell proliferation in hek293t cells |
| topic | <i>FAM49B</i>/<i>Fam49b</i> (CYRI-B) CCND1 cell proliferation CRISPR/Cas9 |
| url | https://www.mdpi.com/2073-4425/13/2/388 |
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