Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens
The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individ...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2002-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800005 |
| _version_ | 1797718491680210944 |
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| author | Antas Paulo RZ Oliveira Eliane B Milagres Alexandre S Franken Kees C Ottenhoff Tom HM Klatser Paul Sarno Euzenir N Sampaio Elizabeth P |
| author_facet | Antas Paulo RZ Oliveira Eliane B Milagres Alexandre S Franken Kees C Ottenhoff Tom HM Klatser Paul Sarno Euzenir N Sampaio Elizabeth P |
| author_sort | Antas Paulo RZ |
| collection | DOAJ |
| description | The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease. |
| first_indexed | 2024-03-12T08:51:31Z |
| format | Article |
| id | doaj.art-04a175b3ade746deacbb9a883124772f |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:51:31Z |
| publishDate | 2002-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-04a175b3ade746deacbb9a883124772f2023-09-02T16:17:51ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602002-01-0197810971099Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigensAntas Paulo RZOliveira Eliane BMilagres Alexandre SFranken Kees COttenhoff Tom HMKlatser PaulSarno Euzenir NSampaio Elizabeth PThe phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800005activation markersCD69CD25flow cytometry |
| spellingShingle | Antas Paulo RZ Oliveira Eliane B Milagres Alexandre S Franken Kees C Ottenhoff Tom HM Klatser Paul Sarno Euzenir N Sampaio Elizabeth P Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens Memorias do Instituto Oswaldo Cruz activation markers CD69 CD25 flow cytometry |
| title | Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens |
| title_full | Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens |
| title_fullStr | Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens |
| title_full_unstemmed | Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens |
| title_short | Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens |
| title_sort | kinetics of t cell activation molecules in response to mycobacterium tuberculosis antigens |
| topic | activation markers CD69 CD25 flow cytometry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800005 |
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