Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism
Myopathies have risen strongly in recent years, likely linked to selection for appetite. For white striping (WS), causes have been identified; but for wooden breast (WB), the cause remains speculative. We used metabolomics to study the breast muscle of 51 birds that were scored for both at 35 days o...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/13/1/20 |
| _version_ | 1797438772118290432 |
|---|---|
| author | Gavin M. Boerboom Alberto Navarro-Villa Theo A. T. G. van Kempen |
| author_facet | Gavin M. Boerboom Alberto Navarro-Villa Theo A. T. G. van Kempen |
| author_sort | Gavin M. Boerboom |
| collection | DOAJ |
| description | Myopathies have risen strongly in recent years, likely linked to selection for appetite. For white striping (WS), causes have been identified; but for wooden breast (WB), the cause remains speculative. We used metabolomics to study the breast muscle of 51 birds that were scored for both at 35 days of age to better understand potential causes. A partial least square discriminant analysis revealed that WS and WB had distinct metabolic profiles, implying different etiologies. Arginine and proline metabolism were affected in both, although differently: WB increased arginine in breast muscle implying that the birds did not use this pathway to increase tissue blood flow. Antioxidant defenses were impeded as shown by low anserine and beta-alanine. In contrast, GSH and selenium concentrations were increased. Serine, linked to anti-inflammatory properties, was increased. Taurine, which can stabilize the cell’s sarcolemma as well as modulate potassium channels and cellular calcium homeostasis, was also increased. Mineral data and depressed phosphatidylethanolamine, cAMP, and creatine-phosphate suggested compromised energy metabolism. WB also had drastically lower diet-derived lipids, suggesting compromised lipid digestion. In conclusion, WB may be caused by impaired lipid digestion triggered by a very high appetite: the ensuing deficiencies may well impair blood flow into muscle resulting in irreparable damage. |
| first_indexed | 2024-03-09T11:43:06Z |
| format | Article |
| id | doaj.art-04a30a58501e42468fd31e8d762b0e8e |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T11:43:06Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-04a30a58501e42468fd31e8d762b0e8e2023-11-30T23:27:44ZengMDPI AGMetabolites2218-19892022-12-011312010.3390/metabo13010020Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid MetabolismGavin M. Boerboom0Alberto Navarro-Villa1Theo A. T. G. van Kempen2Trouw Nutrition R&D, 3811 MH Amersfoort, The NetherlandsTrouw Nutrition R&D, 3811 MH Amersfoort, The NetherlandsDepartment of Animal Science, North Carolina State University, Raleigh, NC 27695, USAMyopathies have risen strongly in recent years, likely linked to selection for appetite. For white striping (WS), causes have been identified; but for wooden breast (WB), the cause remains speculative. We used metabolomics to study the breast muscle of 51 birds that were scored for both at 35 days of age to better understand potential causes. A partial least square discriminant analysis revealed that WS and WB had distinct metabolic profiles, implying different etiologies. Arginine and proline metabolism were affected in both, although differently: WB increased arginine in breast muscle implying that the birds did not use this pathway to increase tissue blood flow. Antioxidant defenses were impeded as shown by low anserine and beta-alanine. In contrast, GSH and selenium concentrations were increased. Serine, linked to anti-inflammatory properties, was increased. Taurine, which can stabilize the cell’s sarcolemma as well as modulate potassium channels and cellular calcium homeostasis, was also increased. Mineral data and depressed phosphatidylethanolamine, cAMP, and creatine-phosphate suggested compromised energy metabolism. WB also had drastically lower diet-derived lipids, suggesting compromised lipid digestion. In conclusion, WB may be caused by impaired lipid digestion triggered by a very high appetite: the ensuing deficiencies may well impair blood flow into muscle resulting in irreparable damage.https://www.mdpi.com/2218-1989/13/1/20broilermetabolomicsmyopathywooden breast |
| spellingShingle | Gavin M. Boerboom Alberto Navarro-Villa Theo A. T. G. van Kempen Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism Metabolites broiler metabolomics myopathy wooden breast |
| title | Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism |
| title_full | Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism |
| title_fullStr | Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism |
| title_full_unstemmed | Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism |
| title_short | Metabolomic Analysis of Wooden Breast Myopathy Shows a Disturbed Lipid Metabolism |
| title_sort | metabolomic analysis of wooden breast myopathy shows a disturbed lipid metabolism |
| topic | broiler metabolomics myopathy wooden breast |
| url | https://www.mdpi.com/2218-1989/13/1/20 |
| work_keys_str_mv | AT gavinmboerboom metabolomicanalysisofwoodenbreastmyopathyshowsadisturbedlipidmetabolism AT albertonavarrovilla metabolomicanalysisofwoodenbreastmyopathyshowsadisturbedlipidmetabolism AT theoatgvankempen metabolomicanalysisofwoodenbreastmyopathyshowsadisturbedlipidmetabolism |