Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China
Juan Xu, 1,* Wenping Lin, 2,* Yanmin Chen, 3 Fang He 3 1Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang 310013, People’s Republic of China; 2Centers for Disease Control and Prevention of Ningbo, Ningbo, Zhejiang 315010, People&...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-03-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/characterization-of-an-imp-4-producing-klebsiella-pneumoniae-st1873-st-peer-reviewed-article-IDR |
| _version_ | 1818594876419211264 |
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| author | Xu J Lin W Chen Y He F |
| author_facet | Xu J Lin W Chen Y He F |
| author_sort | Xu J |
| collection | DOAJ |
| description | Juan Xu, 1,* Wenping Lin, 2,* Yanmin Chen, 3 Fang He 3 1Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang 310013, People’s Republic of China; 2Centers for Disease Control and Prevention of Ningbo, Ningbo, Zhejiang 315010, People’s Republic of China; 3Department of Clinical Laboratory, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fang He Tel/Fax +86-571-8821-5596Email hetrue@163.comPurpose: Imipenemase (IMP), an Ambler class B metallo-β-lactamase, is an important carbapenemase that confers resistance to almost all β-lactams. In this study, we characterized the genomic feature of an IMP-4-producing Klebsiella pneumoniae ST1873 strain, a rare sequence type (ST) isolated from an infant with a bloodstream infection in China.Patients and Methods: K. pneumoniae strain, BKP19, was collected from a bloodstream infection in an infant who was hospitalized at the department of paediatrics. The whole genome sequence of the strain was sequenced using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. Multilocus sequence typing, antimicrobial resistance gene identification, plasmid and phylogenetic relationship analysis of the strain were analysed by various bioinformatics approaches.Results: K. pneumoniae BKP19 was resistant to multiple antimicrobials, including carbapenems. Eleven antimicrobial resistance genes corresponding to beta-lactam resistance, quinolone resistance, phenicol resistance and fosfomycin resistance could be identified in the genome. The carbapenem resistance gene blaIMP-4 was located in an IS26-associated class 1 integron of an IncN-type plasmid with 39,033 bp (pIMP-4-BKP19). Sequence alignment revealed that pIMP-4-BKP19 is closely related to the common plasmid carrying IMP-4 in K. pneumoniae (pIMP-HZ1-like plasmid) but is smaller, lacking the quinolone resistance gene qnrS1 and multiple tra gene orthologs. Conjugation experiment revealed that pIMP-4-BKP19 is a non-conjugative plasmid. According to in silico MLST analysis, K. pneumoniae strain BKP19 belongs to a sporadic clone ST1873.Conclusion: In summary, our study reports the first genome sequence of a K. pneumoniae ST1873 strain harbouring the class B β-lactamase blaIMP-4 in an IncN-type plasmid recovered from an infant with a bloodstream infection in China. Considering the global emergence of IMP-4 carbapenemase, more attention must be paid to prevent its future prevalence.Keywords: Klebsiella pneumoniae, blaIMP-4, IncN plasmid, ST1873, bloodstream infection |
| first_indexed | 2024-12-16T11:07:05Z |
| format | Article |
| id | doaj.art-04a4c791f63e472fb89946f1cf8bdb96 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-16T11:07:05Z |
| publishDate | 2020-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-04a4c791f63e472fb89946f1cf8bdb962022-12-21T22:33:49ZengDove Medical PressInfection and Drug Resistance1178-69732020-03-01Volume 1377377952386Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in ChinaXu JLin WChen YHe FJuan Xu, 1,* Wenping Lin, 2,* Yanmin Chen, 3 Fang He 3 1Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang 310013, People’s Republic of China; 2Centers for Disease Control and Prevention of Ningbo, Ningbo, Zhejiang 315010, People’s Republic of China; 3Department of Clinical Laboratory, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, People’s Republic of China*These authors contributed equally to this workCorrespondence: Fang He Tel/Fax +86-571-8821-5596Email hetrue@163.comPurpose: Imipenemase (IMP), an Ambler class B metallo-β-lactamase, is an important carbapenemase that confers resistance to almost all β-lactams. In this study, we characterized the genomic feature of an IMP-4-producing Klebsiella pneumoniae ST1873 strain, a rare sequence type (ST) isolated from an infant with a bloodstream infection in China.Patients and Methods: K. pneumoniae strain, BKP19, was collected from a bloodstream infection in an infant who was hospitalized at the department of paediatrics. The whole genome sequence of the strain was sequenced using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. Multilocus sequence typing, antimicrobial resistance gene identification, plasmid and phylogenetic relationship analysis of the strain were analysed by various bioinformatics approaches.Results: K. pneumoniae BKP19 was resistant to multiple antimicrobials, including carbapenems. Eleven antimicrobial resistance genes corresponding to beta-lactam resistance, quinolone resistance, phenicol resistance and fosfomycin resistance could be identified in the genome. The carbapenem resistance gene blaIMP-4 was located in an IS26-associated class 1 integron of an IncN-type plasmid with 39,033 bp (pIMP-4-BKP19). Sequence alignment revealed that pIMP-4-BKP19 is closely related to the common plasmid carrying IMP-4 in K. pneumoniae (pIMP-HZ1-like plasmid) but is smaller, lacking the quinolone resistance gene qnrS1 and multiple tra gene orthologs. Conjugation experiment revealed that pIMP-4-BKP19 is a non-conjugative plasmid. According to in silico MLST analysis, K. pneumoniae strain BKP19 belongs to a sporadic clone ST1873.Conclusion: In summary, our study reports the first genome sequence of a K. pneumoniae ST1873 strain harbouring the class B β-lactamase blaIMP-4 in an IncN-type plasmid recovered from an infant with a bloodstream infection in China. Considering the global emergence of IMP-4 carbapenemase, more attention must be paid to prevent its future prevalence.Keywords: Klebsiella pneumoniae, blaIMP-4, IncN plasmid, ST1873, bloodstream infectionhttps://www.dovepress.com/characterization-of-an-imp-4-producing-klebsiella-pneumoniae-st1873-st-peer-reviewed-article-IDRklebsiella pneumoniaeblaimp-4incn plasmidst1873bloodstream infection |
| spellingShingle | Xu J Lin W Chen Y He F Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China Infection and Drug Resistance klebsiella pneumoniae blaimp-4 incn plasmid st1873 bloodstream infection |
| title | Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China |
| title_full | Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China |
| title_fullStr | Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China |
| title_full_unstemmed | Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China |
| title_short | Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China |
| title_sort | characterization of an imp 4 producing klebsiella pneumoniae st1873 strain recovered from an infant with a bloodstream infection in china |
| topic | klebsiella pneumoniae blaimp-4 incn plasmid st1873 bloodstream infection |
| url | https://www.dovepress.com/characterization-of-an-imp-4-producing-klebsiella-pneumoniae-st1873-st-peer-reviewed-article-IDR |
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