The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers.
Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE) and can mediate disease pathogenesis by the formation of immune complexes. Since blocking immune complex formation can attenuate disease manifestations, the effects of nucleic acid binding polymers (NABPs...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3394750?pdf=render |
| _version_ | 1817982232852168704 |
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| author | Nancy A Stearns Jaewoo Lee Kam W Leong Bruce A Sullenger David S Pisetsky |
| author_facet | Nancy A Stearns Jaewoo Lee Kam W Leong Bruce A Sullenger David S Pisetsky |
| author_sort | Nancy A Stearns |
| collection | DOAJ |
| description | Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE) and can mediate disease pathogenesis by the formation of immune complexes. Since blocking immune complex formation can attenuate disease manifestations, the effects of nucleic acid binding polymers (NABPs) on anti-DNA binding in vitro were investigated. The compounds tested included polyamidoamine dendrimer, 1,4-diaminobutane core, generation 3.0 (PAMAM-G3), hexadimethrine bromide, and a β-cylodextrin-containing polycation. As shown with plasma from patients with SLE, NABPs can inhibit anti-DNA antibody binding in ELISA assays. The inhibition was specific since the NABPs did not affect binding to tetanus toxoid or the Sm protein, another lupus autoantigen. Furthermore, the polymers could displace antibody from preformed complexes. Together, these results indicate that NABPs can inhibit the formation of immune complexes and may represent a new approach to treatment. |
| first_indexed | 2024-04-13T23:18:05Z |
| format | Article |
| id | doaj.art-04a52858cab94b828ce2937d1eff4c45 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T23:18:05Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
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| spelling | doaj.art-04a52858cab94b828ce2937d1eff4c452022-12-22T02:25:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4086210.1371/journal.pone.0040862The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers.Nancy A StearnsJaewoo LeeKam W LeongBruce A SullengerDavid S PisetskyAntibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE) and can mediate disease pathogenesis by the formation of immune complexes. Since blocking immune complex formation can attenuate disease manifestations, the effects of nucleic acid binding polymers (NABPs) on anti-DNA binding in vitro were investigated. The compounds tested included polyamidoamine dendrimer, 1,4-diaminobutane core, generation 3.0 (PAMAM-G3), hexadimethrine bromide, and a β-cylodextrin-containing polycation. As shown with plasma from patients with SLE, NABPs can inhibit anti-DNA antibody binding in ELISA assays. The inhibition was specific since the NABPs did not affect binding to tetanus toxoid or the Sm protein, another lupus autoantigen. Furthermore, the polymers could displace antibody from preformed complexes. Together, these results indicate that NABPs can inhibit the formation of immune complexes and may represent a new approach to treatment.http://europepmc.org/articles/PMC3394750?pdf=render |
| spellingShingle | Nancy A Stearns Jaewoo Lee Kam W Leong Bruce A Sullenger David S Pisetsky The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers. PLoS ONE |
| title | The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers. |
| title_full | The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers. |
| title_fullStr | The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers. |
| title_full_unstemmed | The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers. |
| title_short | The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers. |
| title_sort | inhibition of anti dna binding to dna by nucleic acid binding polymers |
| url | http://europepmc.org/articles/PMC3394750?pdf=render |
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