Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from Chickens
The aim of the present study was to investigate variation in antimicrobial resistance in <i>Clostridium perfringens</i> (<i>C. perfringens</i>) isolated from chickens after withdrawal of antimicrobial growth promoters (AGPs); and to investigate the correlation between the pre...
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1825 |
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| author | Bai Wei Se-Yeoun Cha Jun-Feng Zhang Ke Shang Hae-Chul Park JeongWoo Kang Kwang-Jick Lee Min Kang Hyung-Kwan Jang |
| author_facet | Bai Wei Se-Yeoun Cha Jun-Feng Zhang Ke Shang Hae-Chul Park JeongWoo Kang Kwang-Jick Lee Min Kang Hyung-Kwan Jang |
| author_sort | Bai Wei |
| collection | DOAJ |
| description | The aim of the present study was to investigate variation in antimicrobial resistance in <i>Clostridium perfringens</i> (<i>C. perfringens</i>) isolated from chickens after withdrawal of antimicrobial growth promoters (AGPs); and to investigate the correlation between the presence of toxin genes (<i>cpb2</i>, <i>netB</i>, and <i>tpeL</i>) and antimicrobial resistance. Altogether, 162 isolates of <i>C. perfringens</i> were obtained from chickens displaying clinical signs of necrotic enteritis (<i>n</i> = 65) and from healthy chickens (<i>n</i> = 97) in Korea during 2010–2016. Compared to before AGP withdrawal, increased antimicrobial resistance or MIC<sub>50</sub>/MIC<sub>90</sub> value was observed for nine antimicrobials including penicillin, tetracycline, tylosin, erythromycin, florfenicol, enrofloxacin, monensin, salinomycin, and maduramycin. Significantly (<i>p</i> < 0.05) higher resistance to gentamicin, clindamycin, and virginiamycin was found in isolates from chickens with necrotic enteritis compared to those from healthy chickens. <i>tpeL</i> gene was not detected in <i>C. perfringens</i> isolates from healthy chickens. A correlation between toxin gene prevalence and antibiotic resistance was found in the <i>C. perfringens</i> isolates. Because the usage of antimicrobials may contribute to the selection of both resistance and toxin genes, these can potentially make it challenging to control antimicrobial resistance in pathogenic colonies. Therefore, a more complete understanding of the interplay between resistance and virulence genes is required. |
| first_indexed | 2024-03-10T14:43:30Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:43:30Z |
| publishDate | 2020-11-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-04a6acb92e91462b982f963234b2c5bf2023-11-20T21:33:04ZengMDPI AGMicroorganisms2076-26072020-11-01811182510.3390/microorganisms8111825Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from ChickensBai Wei0Se-Yeoun Cha1Jun-Feng Zhang2Ke Shang3Hae-Chul Park4JeongWoo Kang5Kwang-Jick Lee6Min Kang7Hyung-Kwan Jang8Center for Poultry Diseases Control, Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Jeonbuk National University, Iksan 54596, KoreaCenter for Poultry Diseases Control, Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Jeonbuk National University, Iksan 54596, KoreaCenter for Poultry Diseases Control, Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Jeonbuk National University, Iksan 54596, KoreaCenter for Poultry Diseases Control, Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Jeonbuk National University, Iksan 54596, KoreaVeterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA), Gimcheon 39660, KoreaVeterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA), Gimcheon 39660, KoreaVeterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA), Gimcheon 39660, KoreaCenter for Poultry Diseases Control, Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Jeonbuk National University, Iksan 54596, KoreaCenter for Poultry Diseases Control, Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Jeonbuk National University, Iksan 54596, KoreaThe aim of the present study was to investigate variation in antimicrobial resistance in <i>Clostridium perfringens</i> (<i>C. perfringens</i>) isolated from chickens after withdrawal of antimicrobial growth promoters (AGPs); and to investigate the correlation between the presence of toxin genes (<i>cpb2</i>, <i>netB</i>, and <i>tpeL</i>) and antimicrobial resistance. Altogether, 162 isolates of <i>C. perfringens</i> were obtained from chickens displaying clinical signs of necrotic enteritis (<i>n</i> = 65) and from healthy chickens (<i>n</i> = 97) in Korea during 2010–2016. Compared to before AGP withdrawal, increased antimicrobial resistance or MIC<sub>50</sub>/MIC<sub>90</sub> value was observed for nine antimicrobials including penicillin, tetracycline, tylosin, erythromycin, florfenicol, enrofloxacin, monensin, salinomycin, and maduramycin. Significantly (<i>p</i> < 0.05) higher resistance to gentamicin, clindamycin, and virginiamycin was found in isolates from chickens with necrotic enteritis compared to those from healthy chickens. <i>tpeL</i> gene was not detected in <i>C. perfringens</i> isolates from healthy chickens. A correlation between toxin gene prevalence and antibiotic resistance was found in the <i>C. perfringens</i> isolates. Because the usage of antimicrobials may contribute to the selection of both resistance and toxin genes, these can potentially make it challenging to control antimicrobial resistance in pathogenic colonies. Therefore, a more complete understanding of the interplay between resistance and virulence genes is required.https://www.mdpi.com/2076-2607/8/11/1825<i>C. perfringens</i>chickenantimicrobials resistancegrowth promotersvirulence factors |
| spellingShingle | Bai Wei Se-Yeoun Cha Jun-Feng Zhang Ke Shang Hae-Chul Park JeongWoo Kang Kwang-Jick Lee Min Kang Hyung-Kwan Jang Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from Chickens Microorganisms <i>C. perfringens</i> chicken antimicrobials resistance growth promoters virulence factors |
| title | Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from Chickens |
| title_full | Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from Chickens |
| title_fullStr | Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from Chickens |
| title_full_unstemmed | Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from Chickens |
| title_short | Antimicrobial Susceptibility and Association with Toxin Determinants in <i>Clostridium perfringens</i> Isolates from Chickens |
| title_sort | antimicrobial susceptibility and association with toxin determinants in i clostridium perfringens i isolates from chickens |
| topic | <i>C. perfringens</i> chicken antimicrobials resistance growth promoters virulence factors |
| url | https://www.mdpi.com/2076-2607/8/11/1825 |
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