Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced Steatohepatitis
Metabolic-dysfunction-associated fatty-liver disease (MAFLD) is the principal worldwide cause of liver disease. Individuals with nonalcoholic steatohepatitis (NASH) have a higher prevalence of small-intestinal bacterial overgrowth (SIBO). We examined gut-microbiota isolated from 12-week-old stroke-p...
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MDPI AG
2023-02-01
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| author | Shini Kanezawa Mitsuhiko Moriyama Tatsuo Kanda Akiko Fukushima Ryota Masuzaki Reina Sasaki-Tanaka Akiko Tsunemi Takahiro Ueno Noboru Fukuda Hirofumi Kogure |
| author_facet | Shini Kanezawa Mitsuhiko Moriyama Tatsuo Kanda Akiko Fukushima Ryota Masuzaki Reina Sasaki-Tanaka Akiko Tsunemi Takahiro Ueno Noboru Fukuda Hirofumi Kogure |
| author_sort | Shini Kanezawa |
| collection | DOAJ |
| description | Metabolic-dysfunction-associated fatty-liver disease (MAFLD) is the principal worldwide cause of liver disease. Individuals with nonalcoholic steatohepatitis (NASH) have a higher prevalence of small-intestinal bacterial overgrowth (SIBO). We examined gut-microbiota isolated from 12-week-old stroke-prone spontaneously hypertensive-5 rats (SHRSP5) fed on a normal diet (ND) or a high-fat- and high-cholesterol-containing diet (HFCD) and clarified the differences between their gut-microbiota. We observed that the <i>Firmicute/Bacteroidetes (F/B)</i> ratio in both the small intestines and the feces of the SHRSP5 rats fed HFCD increased compared to that of the SHRSP5 rats fed ND. Notably, the quantities of the 16S rRNA genes in small intestines of the SHRSP5 rats fed HFCD were significantly lower than those of the SHRSP5 rats fed ND. As in SIBO syndrome, the SHRSP5 rats fed HFCD presented with diarrhea and body-weight loss with abnormal types of bacteria in the small intestine, although the number of bacteria in the small intestine did not increase. The microbiota of the feces in the SHRSP5 rats fed HFCD was different from those in the SHRP5 rats fed ND. In conclusion, there is an association between MAFLD and gut-microbiota alteration. Gut-microbiota alteration may be a therapeutic target for MAFLD. |
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| language | English |
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| spelling | doaj.art-04a70fb8fc8540c0b61bdd97322acce42023-11-17T07:50:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01245460310.3390/ijms24054603Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced SteatohepatitisShini Kanezawa0Mitsuhiko Moriyama1Tatsuo Kanda2Akiko Fukushima3Ryota Masuzaki4Reina Sasaki-Tanaka5Akiko Tsunemi6Takahiro Ueno7Noboru Fukuda8Hirofumi Kogure9Division of Gastroenterology and Hepatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Gastroenterology and Hepatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Gastroenterology and Hepatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Gastroenterology and Hepatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Gastroenterology and Hepatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Gastroenterology and Hepatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanDivision of Gastroenterology and Hepatology, Department of Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, JapanMetabolic-dysfunction-associated fatty-liver disease (MAFLD) is the principal worldwide cause of liver disease. Individuals with nonalcoholic steatohepatitis (NASH) have a higher prevalence of small-intestinal bacterial overgrowth (SIBO). We examined gut-microbiota isolated from 12-week-old stroke-prone spontaneously hypertensive-5 rats (SHRSP5) fed on a normal diet (ND) or a high-fat- and high-cholesterol-containing diet (HFCD) and clarified the differences between their gut-microbiota. We observed that the <i>Firmicute/Bacteroidetes (F/B)</i> ratio in both the small intestines and the feces of the SHRSP5 rats fed HFCD increased compared to that of the SHRSP5 rats fed ND. Notably, the quantities of the 16S rRNA genes in small intestines of the SHRSP5 rats fed HFCD were significantly lower than those of the SHRSP5 rats fed ND. As in SIBO syndrome, the SHRSP5 rats fed HFCD presented with diarrhea and body-weight loss with abnormal types of bacteria in the small intestine, although the number of bacteria in the small intestine did not increase. The microbiota of the feces in the SHRSP5 rats fed HFCD was different from those in the SHRP5 rats fed ND. In conclusion, there is an association between MAFLD and gut-microbiota alteration. Gut-microbiota alteration may be a therapeutic target for MAFLD.https://www.mdpi.com/1422-0067/24/5/4603high-fat- and high-cholesterol-containing diet<i>Firmicutes/Bacteroidetes</i> ratiogut-microbiotametabolic-dysfunction-associated fatty-liver diseasenonalcoholic steatohepatitissmall-intestinal bacterial overgrowth |
| spellingShingle | Shini Kanezawa Mitsuhiko Moriyama Tatsuo Kanda Akiko Fukushima Ryota Masuzaki Reina Sasaki-Tanaka Akiko Tsunemi Takahiro Ueno Noboru Fukuda Hirofumi Kogure Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced Steatohepatitis International Journal of Molecular Sciences high-fat- and high-cholesterol-containing diet <i>Firmicutes/Bacteroidetes</i> ratio gut-microbiota metabolic-dysfunction-associated fatty-liver disease nonalcoholic steatohepatitis small-intestinal bacterial overgrowth |
| title | Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced Steatohepatitis |
| title_full | Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced Steatohepatitis |
| title_fullStr | Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced Steatohepatitis |
| title_full_unstemmed | Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced Steatohepatitis |
| title_short | Gut-Microbiota Dysbiosis in Stroke-Prone Spontaneously Hypertensive Rats with Diet-Induced Steatohepatitis |
| title_sort | gut microbiota dysbiosis in stroke prone spontaneously hypertensive rats with diet induced steatohepatitis |
| topic | high-fat- and high-cholesterol-containing diet <i>Firmicutes/Bacteroidetes</i> ratio gut-microbiota metabolic-dysfunction-associated fatty-liver disease nonalcoholic steatohepatitis small-intestinal bacterial overgrowth |
| url | https://www.mdpi.com/1422-0067/24/5/4603 |
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