Genetic diversity in cultivated carioca common beans based on molecular marker analysis
A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca be...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2011-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017 |
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| author | Juliana Morini Küpper Cardoso Perseguini Alisson Fernando Chioratto Maria Imaculada Zucchi Carlos Augusto Colombo Sérgio Augusto Moraes Carbonell Jorge Mauricio Costa Mondego Rodrigo Gazaffi Antonio Augusto Franco Garcia Tatiana de Campos Anete Pereira de Souza Luciana Benchimol Rubiano |
| author_facet | Juliana Morini Küpper Cardoso Perseguini Alisson Fernando Chioratto Maria Imaculada Zucchi Carlos Augusto Colombo Sérgio Augusto Moraes Carbonell Jorge Mauricio Costa Mondego Rodrigo Gazaffi Antonio Augusto Franco Garcia Tatiana de Campos Anete Pereira de Souza Luciana Benchimol Rubiano |
| author_sort | Juliana Morini Küpper Cardoso Perseguini |
| collection | DOAJ |
| description | A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm. |
| first_indexed | 2024-12-22T03:19:14Z |
| format | Article |
| id | doaj.art-04a73e63a7d84660904e12fb71d5676b |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T03:19:14Z |
| publishDate | 2011-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-04a73e63a7d84660904e12fb71d5676b2022-12-21T18:40:45ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-013418810210.1590/S1415-47572011000100017Genetic diversity in cultivated carioca common beans based on molecular marker analysisJuliana Morini Küpper Cardoso PerseguiniAlisson Fernando ChiorattoMaria Imaculada ZucchiCarlos Augusto ColomboSérgio Augusto Moraes CarbonellJorge Mauricio Costa MondegoRodrigo GazaffiAntonio Augusto Franco GarciaTatiana de CamposAnete Pereira de SouzaLuciana Benchimol RubianoA wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017AFLPsgenetic structuregenetic variabilityPhaseolus vulgaris L.SSRs |
| spellingShingle | Juliana Morini Küpper Cardoso Perseguini Alisson Fernando Chioratto Maria Imaculada Zucchi Carlos Augusto Colombo Sérgio Augusto Moraes Carbonell Jorge Mauricio Costa Mondego Rodrigo Gazaffi Antonio Augusto Franco Garcia Tatiana de Campos Anete Pereira de Souza Luciana Benchimol Rubiano Genetic diversity in cultivated carioca common beans based on molecular marker analysis Genetics and Molecular Biology AFLPs genetic structure genetic variability Phaseolus vulgaris L. SSRs |
| title | Genetic diversity in cultivated carioca common beans based on molecular marker analysis |
| title_full | Genetic diversity in cultivated carioca common beans based on molecular marker analysis |
| title_fullStr | Genetic diversity in cultivated carioca common beans based on molecular marker analysis |
| title_full_unstemmed | Genetic diversity in cultivated carioca common beans based on molecular marker analysis |
| title_short | Genetic diversity in cultivated carioca common beans based on molecular marker analysis |
| title_sort | genetic diversity in cultivated carioca common beans based on molecular marker analysis |
| topic | AFLPs genetic structure genetic variability Phaseolus vulgaris L. SSRs |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100017 |
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