A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape Pomace
Antioxidant compounds with health benefits can be found in food processing residues, such as grape pomace. In this study, antioxidants were identified and quantified in an extract obtained from Graciano red grape pomace via a green process. The antioxidant activity of the extract was assessed by the...
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| Formatua: | Artikulua |
| Hizkuntza: | English |
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MDPI AG
2022-06-01
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| Saila: | Antioxidants |
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| Sarrera elektronikoa: | https://www.mdpi.com/2076-3921/11/7/1270 |
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| author | Carlos Asensio-Regalado Rosa María Alonso-Salces Blanca Gallo Luis A. Berrueta Clara Porcedda Francesca Pintus Antonio Vassallo Carla Caddeo |
| author_facet | Carlos Asensio-Regalado Rosa María Alonso-Salces Blanca Gallo Luis A. Berrueta Clara Porcedda Francesca Pintus Antonio Vassallo Carla Caddeo |
| author_sort | Carlos Asensio-Regalado |
| collection | DOAJ |
| description | Antioxidant compounds with health benefits can be found in food processing residues, such as grape pomace. In this study, antioxidants were identified and quantified in an extract obtained from Graciano red grape pomace via a green process. The antioxidant activity of the extract was assessed by the DPPH and FRAP tests, and the phenolic content by the Folin–Ciocalteu test. Furthermore, nanotechnologies were employed to produce a safe and effective formulation that would exploit the antioxidant potential of the extract for skin applications. Anthocyanins, flavan-3-ols and flavanols were the main constituents of the grape pomace extract. Phospholipid vesicles, namely liposomes, were prepared and characterized. Cryo-TEM images showed that the extract-loaded liposomes were predominantly spherical/elongated, small, unilamellar vesicles. Light scattering results revealed that the liposomes were small (~100 nm), homogeneously dispersed, and stable during storage. The non-toxicity of the liposomal formulation was demonstrated in vitro in skin cells, suggesting its possible safe use. These findings indicate that an extract with antioxidant properties can be obtained from food processing residues, and a liposomal formulation can be developed to exploit its bioactive value, resulting in a promising healthy product. |
| first_indexed | 2024-03-09T10:22:49Z |
| format | Article |
| id | doaj.art-04a85834fb2f4fa38c1e10eeab1a6d1c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T10:22:49Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-04a85834fb2f4fa38c1e10eeab1a6d1c2023-12-01T21:49:36ZengMDPI AGAntioxidants2076-39212022-06-01117127010.3390/antiox11071270A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape PomaceCarlos Asensio-Regalado0Rosa María Alonso-Salces1Blanca Gallo2Luis A. Berrueta3Clara Porcedda4Francesca Pintus5Antonio Vassallo6Carla Caddeo7Department of Analytical Chemistry, University of the Basque Country UPV/EHU, P.O. Box 644, 48080 Bilbao, SpainConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CIAS-IIPROSAM, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3350, Mar de Plata 7600, ArgentinaDepartment of Analytical Chemistry, University of the Basque Country UPV/EHU, P.O. Box 644, 48080 Bilbao, SpainDepartment of Analytical Chemistry, University of the Basque Country UPV/EHU, P.O. Box 644, 48080 Bilbao, SpainDepartment of Biomedical Sciences, University of Cagliari, SS 554-Bivio per Sestu, Monserrato, 09042 Cagliari, ItalyDepartment of Scienze della Vita e dell’Ambiente, Sezione Biomedica, University of Cagliari, SS 554-Bivio per Sestu, 09042 Cagliari, ItalyDepartment of Science, University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyDepartment of Scienze della Vita e dell’Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, ItalyAntioxidant compounds with health benefits can be found in food processing residues, such as grape pomace. In this study, antioxidants were identified and quantified in an extract obtained from Graciano red grape pomace via a green process. The antioxidant activity of the extract was assessed by the DPPH and FRAP tests, and the phenolic content by the Folin–Ciocalteu test. Furthermore, nanotechnologies were employed to produce a safe and effective formulation that would exploit the antioxidant potential of the extract for skin applications. Anthocyanins, flavan-3-ols and flavanols were the main constituents of the grape pomace extract. Phospholipid vesicles, namely liposomes, were prepared and characterized. Cryo-TEM images showed that the extract-loaded liposomes were predominantly spherical/elongated, small, unilamellar vesicles. Light scattering results revealed that the liposomes were small (~100 nm), homogeneously dispersed, and stable during storage. The non-toxicity of the liposomal formulation was demonstrated in vitro in skin cells, suggesting its possible safe use. These findings indicate that an extract with antioxidant properties can be obtained from food processing residues, and a liposomal formulation can be developed to exploit its bioactive value, resulting in a promising healthy product.https://www.mdpi.com/2076-3921/11/7/1270grape pomacegreen extractionliposomesantioxidantskin cellscytocompatibility |
| spellingShingle | Carlos Asensio-Regalado Rosa María Alonso-Salces Blanca Gallo Luis A. Berrueta Clara Porcedda Francesca Pintus Antonio Vassallo Carla Caddeo A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape Pomace Antioxidants grape pomace green extraction liposomes antioxidant skin cells cytocompatibility |
| title | A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape Pomace |
| title_full | A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape Pomace |
| title_fullStr | A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape Pomace |
| title_full_unstemmed | A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape Pomace |
| title_short | A Liposomal Formulation to Exploit the Bioactive Potential of an Extract from <i>Graciano</i> Grape Pomace |
| title_sort | liposomal formulation to exploit the bioactive potential of an extract from i graciano i grape pomace |
| topic | grape pomace green extraction liposomes antioxidant skin cells cytocompatibility |
| url | https://www.mdpi.com/2076-3921/11/7/1270 |
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