Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles
Ascoviruses are large DNA viruses that primarily infect lepidopteran larvae. They differ markedly from other plant or animal viruses by initiating replication in the nucleus, then inducing nuclear lysis followed by extensive cellular hypertrophy and subsequent cleavage of the entire enlarged cell in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/7/1444 |
| _version_ | 1797415072427933696 |
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| author | Heba A. H. Zaghloul Peter Arensburger Brian A. Federici |
| author_facet | Heba A. H. Zaghloul Peter Arensburger Brian A. Federici |
| author_sort | Heba A. H. Zaghloul |
| collection | DOAJ |
| description | Ascoviruses are large DNA viruses that primarily infect lepidopteran larvae. They differ markedly from other plant or animal viruses by initiating replication in the nucleus, then inducing nuclear lysis followed by extensive cellular hypertrophy and subsequent cleavage of the entire enlarged cell into numerous viral vesicles. Most progeny virions are assembled in these vesicles as they circulate in the hemolymph. Here, we report transcriptome studies of host cytoskeletal genes in larvae infected with ascoviruses from 6 h to 21 days post-infection (dpi). We focused on the cabbage looper, <i>Trichoplusia ni</i>, infected with the <i>Trichoplusia ni</i> ascovirus (TnAV), along with supporting studies on the fall armyworm, <i>Spodoptera frugiperda</i>, infected with the <i>Spodoptera frugiperda</i> ascovirus (SfAV). In <i>T. ni</i>, many cytoskeleton genes were upregulated at 48 hours post-infection (hpi), including 29 tubulins, 21 actins, 21 dyneins, and 13 kinesins. Mitochondrial genes were upregulated as much as two-fold at 48 hpi and were expressed at levels comparable to controls in both <i>T. ni</i> and <i>S. frugiperda</i>, even after 21 dpi, when several cytoskeleton genes remained upregulated. Our studies suggest a temporal correlation between increases in the expression of certain host cytoskeletal genes and viral vesicle formation. However, these results need confirmation through functional genetic studies of proteins encoded by these genes. |
| first_indexed | 2024-03-09T05:42:46Z |
| format | Article |
| id | doaj.art-04b3689beb0d4cb9a7354931dbefb24f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:42:46Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-04b3689beb0d4cb9a7354931dbefb24f2023-12-03T12:23:07ZengMDPI AGViruses1999-49152022-06-01147144410.3390/v14071444Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral VesiclesHeba A. H. Zaghloul0Peter Arensburger1Brian A. Federici2Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria 21511, EgyptDepartment of Biological Sciences, California State Polytechnic University, Pomona, 3801 West Temple Avenue, Pomona, CA 91768, USAInterdepartmental Graduate Program in Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USAAscoviruses are large DNA viruses that primarily infect lepidopteran larvae. They differ markedly from other plant or animal viruses by initiating replication in the nucleus, then inducing nuclear lysis followed by extensive cellular hypertrophy and subsequent cleavage of the entire enlarged cell into numerous viral vesicles. Most progeny virions are assembled in these vesicles as they circulate in the hemolymph. Here, we report transcriptome studies of host cytoskeletal genes in larvae infected with ascoviruses from 6 h to 21 days post-infection (dpi). We focused on the cabbage looper, <i>Trichoplusia ni</i>, infected with the <i>Trichoplusia ni</i> ascovirus (TnAV), along with supporting studies on the fall armyworm, <i>Spodoptera frugiperda</i>, infected with the <i>Spodoptera frugiperda</i> ascovirus (SfAV). In <i>T. ni</i>, many cytoskeleton genes were upregulated at 48 hours post-infection (hpi), including 29 tubulins, 21 actins, 21 dyneins, and 13 kinesins. Mitochondrial genes were upregulated as much as two-fold at 48 hpi and were expressed at levels comparable to controls in both <i>T. ni</i> and <i>S. frugiperda</i>, even after 21 dpi, when several cytoskeleton genes remained upregulated. Our studies suggest a temporal correlation between increases in the expression of certain host cytoskeletal genes and viral vesicle formation. However, these results need confirmation through functional genetic studies of proteins encoded by these genes.https://www.mdpi.com/1999-4915/14/7/1444ascoviruscytoskeletonmitochondriaRNA-seqvesicles<i>Trichoplusia ni</i> |
| spellingShingle | Heba A. H. Zaghloul Peter Arensburger Brian A. Federici Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles Viruses ascovirus cytoskeleton mitochondria RNA-seq vesicles <i>Trichoplusia ni</i> |
| title | Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles |
| title_full | Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles |
| title_fullStr | Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles |
| title_full_unstemmed | Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles |
| title_short | Host Cytoskeleton Gene Expression Is Correlated with the Formation of Ascovirus Reproductive Viral Vesicles |
| title_sort | host cytoskeleton gene expression is correlated with the formation of ascovirus reproductive viral vesicles |
| topic | ascovirus cytoskeleton mitochondria RNA-seq vesicles <i>Trichoplusia ni</i> |
| url | https://www.mdpi.com/1999-4915/14/7/1444 |
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