Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy
In this paper, ZnS nanoparticles were bioconjugated with bovine serum albumin and prepared in a form of nanosuspension using a wet circulation grinding. The stable nanosuspension with monomodal particle size distribution (d<sub>50</sub> = 137 nm) and negative zeta potential (−18.3 mV) wa...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/16/9/1219 |
| _version_ | 1797578067432964096 |
|---|---|
| author | Zdenka Lukáčová Bujňáková Erika Dutková Jana Jakubíková Danka Cholujová Rastislav Varhač Larysa Borysenko Inna Melnyk |
| author_facet | Zdenka Lukáčová Bujňáková Erika Dutková Jana Jakubíková Danka Cholujová Rastislav Varhač Larysa Borysenko Inna Melnyk |
| author_sort | Zdenka Lukáčová Bujňáková |
| collection | DOAJ |
| description | In this paper, ZnS nanoparticles were bioconjugated with bovine serum albumin and prepared in a form of nanosuspension using a wet circulation grinding. The stable nanosuspension with monomodal particle size distribution (d<sub>50</sub> = 137 nm) and negative zeta potential (−18.3 mV) was obtained. The sorption kinetics and isotherm were determined. Interactions between ZnS and albumin were studied using the fluorescence techniques. The quenching mechanism, describing both static and dynamic interactions, was investigated. Various parameters were calculated, including the quenching rate constant, binding constant, stoichiometry of the binding process, and accessibility of fluorophore to the quencher. It has been found that tryptophan, in comparison to tyrosine, can be closer to the binding site established by analyzing the synchronous fluorescence spectra. The cellular mechanism in multiple myeloma cells treated with nanosuspension was evaluated by fluorescence assays for quantification of apoptosis, assessment of mitochondrial membrane potential and evaluation of cell cycle changes. The preliminary results confirm that the nontoxic nature of ZnS nanoparticles is potentially applicable in drug delivery systems. Additionally, slight changes in the secondary structure of albumin, accompanied by a decrease in α-helix content, were investigated using the FTIR method after analyzing the deconvoluted Amide I band spectra of ZnS nanoparticles conjugated with albumin. Thermogravimetric analysis and long-term stability studies were also performed to obtain a complete picture about the studied system. |
| first_indexed | 2024-03-10T22:17:24Z |
| format | Article |
| id | doaj.art-04c06157258346c8822288ee92f40601 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T22:17:24Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-04c06157258346c8822288ee92f406012023-11-19T12:24:13ZengMDPI AGPharmaceuticals1424-82472023-08-01169121910.3390/ph16091219Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence SpectroscopyZdenka Lukáčová Bujňáková0Erika Dutková1Jana Jakubíková2Danka Cholujová3Rastislav Varhač4Larysa Borysenko5Inna Melnyk6Institute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, 04001 Košice, SlovakiaInstitute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, 04001 Košice, SlovakiaCancer Research Institute of Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 84505 Bratislava, SlovakiaCancer Research Institute of Biomedical Research Center, Slovak Academy of Sciences, Dúbravská Cesta 9, 84505 Bratislava, SlovakiaFaculty of Science, Pavol Jozef Šafárik University in Košice, Šrobárova 2, 04154 Košice, SlovakiaChuiko Institute of Surface Chemistry, National Academy of Science of Ukraine, Generala Naumova 17, 03164 Kyiv, UkraineInstitute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, 04001 Košice, SlovakiaIn this paper, ZnS nanoparticles were bioconjugated with bovine serum albumin and prepared in a form of nanosuspension using a wet circulation grinding. The stable nanosuspension with monomodal particle size distribution (d<sub>50</sub> = 137 nm) and negative zeta potential (−18.3 mV) was obtained. The sorption kinetics and isotherm were determined. Interactions between ZnS and albumin were studied using the fluorescence techniques. The quenching mechanism, describing both static and dynamic interactions, was investigated. Various parameters were calculated, including the quenching rate constant, binding constant, stoichiometry of the binding process, and accessibility of fluorophore to the quencher. It has been found that tryptophan, in comparison to tyrosine, can be closer to the binding site established by analyzing the synchronous fluorescence spectra. The cellular mechanism in multiple myeloma cells treated with nanosuspension was evaluated by fluorescence assays for quantification of apoptosis, assessment of mitochondrial membrane potential and evaluation of cell cycle changes. The preliminary results confirm that the nontoxic nature of ZnS nanoparticles is potentially applicable in drug delivery systems. Additionally, slight changes in the secondary structure of albumin, accompanied by a decrease in α-helix content, were investigated using the FTIR method after analyzing the deconvoluted Amide I band spectra of ZnS nanoparticles conjugated with albumin. Thermogravimetric analysis and long-term stability studies were also performed to obtain a complete picture about the studied system.https://www.mdpi.com/1424-8247/16/9/1219ZnS nanoparticlesalbuminmillingfluorescencequenching |
| spellingShingle | Zdenka Lukáčová Bujňáková Erika Dutková Jana Jakubíková Danka Cholujová Rastislav Varhač Larysa Borysenko Inna Melnyk Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy Pharmaceuticals ZnS nanoparticles albumin milling fluorescence quenching |
| title | Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy |
| title_full | Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy |
| title_fullStr | Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy |
| title_full_unstemmed | Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy |
| title_short | Investigation of the Interaction between Mechanosynthesized ZnS Nanoparticles and Albumin Using Fluorescence Spectroscopy |
| title_sort | investigation of the interaction between mechanosynthesized zns nanoparticles and albumin using fluorescence spectroscopy |
| topic | ZnS nanoparticles albumin milling fluorescence quenching |
| url | https://www.mdpi.com/1424-8247/16/9/1219 |
| work_keys_str_mv | AT zdenkalukacovabujnakova investigationoftheinteractionbetweenmechanosynthesizedznsnanoparticlesandalbuminusingfluorescencespectroscopy AT erikadutkova investigationoftheinteractionbetweenmechanosynthesizedznsnanoparticlesandalbuminusingfluorescencespectroscopy AT janajakubikova investigationoftheinteractionbetweenmechanosynthesizedznsnanoparticlesandalbuminusingfluorescencespectroscopy AT dankacholujova investigationoftheinteractionbetweenmechanosynthesizedznsnanoparticlesandalbuminusingfluorescencespectroscopy AT rastislavvarhac investigationoftheinteractionbetweenmechanosynthesizedznsnanoparticlesandalbuminusingfluorescencespectroscopy AT larysaborysenko investigationoftheinteractionbetweenmechanosynthesizedznsnanoparticlesandalbuminusingfluorescencespectroscopy AT innamelnyk investigationoftheinteractionbetweenmechanosynthesizedznsnanoparticlesandalbuminusingfluorescencespectroscopy |