Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray
In spite of intensive and increasingly successful attempts to determine the multiple steps involved in colorectal carcinogenesis, the mechanisms responsible for metastasis of colorectal tumors to the liver remain to be clarified. To identify genes that are candidates for involvement in the metastati...
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| Format: | Article |
| Language: | English |
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Elsevier
2001-01-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S147655860180052X |
| _version_ | 1811316513148239872 |
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| author | Rempei Yanagawa Yoichi Furukawa Tatsuhiko Tsunoda Osamu Kitahara Masao Kameyama Kohei Murata Osamu Ishikawa Yusuke Nakamura |
| author_facet | Rempei Yanagawa Yoichi Furukawa Tatsuhiko Tsunoda Osamu Kitahara Masao Kameyama Kohei Murata Osamu Ishikawa Yusuke Nakamura |
| author_sort | Rempei Yanagawa |
| collection | DOAJ |
| description | In spite of intensive and increasingly successful attempts to determine the multiple steps involved in colorectal carcinogenesis, the mechanisms responsible for metastasis of colorectal tumors to the liver remain to be clarified. To identify genes that are candidates for involvement in the metastatic process, we analyzed genome-wide expression profiles of 10 primary colorectal cancers and their corresponding metastatic lesions by means of a cDNA microarray consisting of 9121 human genes. This analysis identified 40 genes whose expression was commonly upregulated in metastatic lesions, and 7 that were commonly downregulated. The upregulated genes encoded proteins involved in cell adhesion, or remodeling of the actin cytoskeleton. Investigation of the functions of more of the altered genes should improve our understanding of metastasis and may identify diagnostic markers and/or novel molecular targets for prevention or therapy of metastatic lesions. |
| first_indexed | 2024-04-13T11:50:08Z |
| format | Article |
| id | doaj.art-04c28dffea444b558f93704a06ced051 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-04-13T11:50:08Z |
| publishDate | 2001-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-04c28dffea444b558f93704a06ced0512022-12-22T02:48:03ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022001-01-013539540110.1038/sj.neo.7900185Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA MicroarrayRempei Yanagawa0Yoichi Furukawa1Tatsuhiko Tsunoda2Osamu Kitahara3Masao Kameyama4Kohei Murata5Osamu Ishikawa6Yusuke Nakamura7Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanLaboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanLaboratory for Medical Informatics, SNP Research Center, Riken (Institute of Physical and Chemical Research), 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanLaboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanDepartment of Surgical Oncology, Osaka Medical Center for Cancer and Cardiovascular Disease, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, JapanDepartment of Surgical Oncology, Osaka Medical Center for Cancer and Cardiovascular Disease, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, JapanDepartment of Surgical Oncology, Osaka Medical Center for Cancer and Cardiovascular Disease, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, JapanLaboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanIn spite of intensive and increasingly successful attempts to determine the multiple steps involved in colorectal carcinogenesis, the mechanisms responsible for metastasis of colorectal tumors to the liver remain to be clarified. To identify genes that are candidates for involvement in the metastatic process, we analyzed genome-wide expression profiles of 10 primary colorectal cancers and their corresponding metastatic lesions by means of a cDNA microarray consisting of 9121 human genes. This analysis identified 40 genes whose expression was commonly upregulated in metastatic lesions, and 7 that were commonly downregulated. The upregulated genes encoded proteins involved in cell adhesion, or remodeling of the actin cytoskeleton. Investigation of the functions of more of the altered genes should improve our understanding of metastasis and may identify diagnostic markers and/or novel molecular targets for prevention or therapy of metastatic lesions.http://www.sciencedirect.com/science/article/pii/S147655860180052XmetastasiscDNA microarraymicrodissectioncolorectal cancer (CRC)T7-based RNA amplification |
| spellingShingle | Rempei Yanagawa Yoichi Furukawa Tatsuhiko Tsunoda Osamu Kitahara Masao Kameyama Kohei Murata Osamu Ishikawa Yusuke Nakamura Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray Neoplasia: An International Journal for Oncology Research metastasis cDNA microarray microdissection colorectal cancer (CRC) T7-based RNA amplification |
| title | Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray |
| title_full | Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray |
| title_fullStr | Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray |
| title_full_unstemmed | Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray |
| title_short | Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray |
| title_sort | genome wide screening of genes showing altered expression in liver metastases of human colorectal cancers by cdna microarray |
| topic | metastasis cDNA microarray microdissection colorectal cancer (CRC) T7-based RNA amplification |
| url | http://www.sciencedirect.com/science/article/pii/S147655860180052X |
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