Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Ce...
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| Format: | Article |
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MDPI AG
2015-05-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/7/5/1738 |
| _version_ | 1818474041836568576 |
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| author | Lina Lu Zhijun Qi Jiwen Zhang Wenjun Wu |
| author_facet | Lina Lu Zhijun Qi Jiwen Zhang Wenjun Wu |
| author_sort | Lina Lu |
| collection | DOAJ |
| description | Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V. Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae. The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed. |
| first_indexed | 2024-04-14T04:31:17Z |
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| id | doaj.art-04c4ab041c9249b699ffa0801fafb08f |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T04:31:17Z |
| publishDate | 2015-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-04c4ab041c9249b699ffa0801fafb08f2022-12-22T02:12:01ZengMDPI AGToxins2072-66512015-05-01751738174810.3390/toxins7051738toxins7051738Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity ChromatographyLina Lu0Zhijun Qi1Jiwen Zhang2Wenjun Wu3Institute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, ChinaInstitute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, ChinaCollege of Science, Northwest A & F University, Yangling 712100, Shaanxi, ChinaInstitute of Pesticide Science, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, ChinaCelangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to analyze the collected fraction eluted by Celangulin V. Eight binding proteins (Zinc finger protein, Thioredoxin peroxidase (TPx), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), SUMO E3 ligase RanBP2, Transmembrane protein 1, Actin, APN and V-ATPase) were obtained and identified by LC/Q-TOF-MS from the midgut of Mythimna separata larvae. The potential of these proteins to serve as target proteins involved in the insecticidal activity of Celangulin V is discussed.http://www.mdpi.com/2072-6651/7/5/1738Celangulin Vbinding proteinaffinity chromatographyMythimna separata Walker |
| spellingShingle | Lina Lu Zhijun Qi Jiwen Zhang Wenjun Wu Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography Toxins Celangulin V binding protein affinity chromatography Mythimna separata Walker |
| title | Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography |
| title_full | Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography |
| title_fullStr | Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography |
| title_full_unstemmed | Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography |
| title_short | Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography |
| title_sort | separation of binding protein of celangulin v from the midgut of mythimna separata walker by affinity chromatography |
| topic | Celangulin V binding protein affinity chromatography Mythimna separata Walker |
| url | http://www.mdpi.com/2072-6651/7/5/1738 |
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