Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens
<p>To investigate the effects of miR-499-5p on muscle metabolism in broiler chickens, eight broiler chicks were assigned to the control group and eight to the treatment group, and then we monitored the effects using metabolomics. Chicks were fed basal diets without or with miR-499-5p delivery....
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2022-08-01
|
| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/65/275/2022/aab-65-275-2022.pdf |
| _version_ | 1828187322292633600 |
|---|---|
| author | C. Chen C. Chen J. Li J. Li Z. Li Z. Li |
| author_facet | C. Chen C. Chen J. Li J. Li Z. Li Z. Li |
| author_sort | C. Chen |
| collection | DOAJ |
| description | <p>To investigate the effects of miR-499-5p on muscle
metabolism in broiler chickens, eight broiler chicks were assigned to the
control group and eight to the treatment group, and then we monitored the effects
using metabolomics. Chicks were fed basal diets without or with miR-499-5p
delivery. Gastrocnemius muscle samples were collected and analyzed by
ultrahigh-performance liquid chromatography–tandem mass spectrometry. The
results showed that miR-499-5p injection altered the concentrations of a
variety of metabolites in the gastrocnemius muscle. Thereby, a total of 46
metabolites were identified at higher (<span class="inline-formula"><i>P</i><i><</i>0.05</span>) concentrations and
30 metabolites were identified at lower (<span class="inline-formula"><i>P</i><i><</i>0.05</span>) concentrations in
the treatment group compared with the control group. These metabolites
were primarily involved with the regulation of lipid and carbohydrate
metabolism. Further metabolic pathway analysis revealed that fructose and
mannose metabolism, galactose metabolism, inositol phosphate metabolism, and
terpenoid backbone biosynthesis were the most critical pathway which may
partially interpret the effects of miR-499-5p. To our knowledge, this
research is the first report of metabolic signatures and related metabolic
pathways in the skeletal muscle for miR-499-5p injection and provides new
insight into the effect of miRNA on growth performance.</p> |
| first_indexed | 2024-04-12T07:34:12Z |
| format | Article |
| id | doaj.art-04c537faf1ec4499941ebd0528664b4b |
| institution | Directory Open Access Journal |
| issn | 0003-9438 2363-9822 |
| language | English |
| last_indexed | 2024-04-12T07:34:12Z |
| publishDate | 2022-08-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj.art-04c537faf1ec4499941ebd0528664b4b2022-12-22T03:41:59ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222022-08-016527528410.5194/aab-65-275-2022Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickensC. Chen0C. Chen1J. Li2J. Li3Z. Li4Z. Li5Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, 610041, ChinaCollege of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, 610041, ChinaCollege of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, 610041, ChinaCollege of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China<p>To investigate the effects of miR-499-5p on muscle metabolism in broiler chickens, eight broiler chicks were assigned to the control group and eight to the treatment group, and then we monitored the effects using metabolomics. Chicks were fed basal diets without or with miR-499-5p delivery. Gastrocnemius muscle samples were collected and analyzed by ultrahigh-performance liquid chromatography–tandem mass spectrometry. The results showed that miR-499-5p injection altered the concentrations of a variety of metabolites in the gastrocnemius muscle. Thereby, a total of 46 metabolites were identified at higher (<span class="inline-formula"><i>P</i><i><</i>0.05</span>) concentrations and 30 metabolites were identified at lower (<span class="inline-formula"><i>P</i><i><</i>0.05</span>) concentrations in the treatment group compared with the control group. These metabolites were primarily involved with the regulation of lipid and carbohydrate metabolism. Further metabolic pathway analysis revealed that fructose and mannose metabolism, galactose metabolism, inositol phosphate metabolism, and terpenoid backbone biosynthesis were the most critical pathway which may partially interpret the effects of miR-499-5p. To our knowledge, this research is the first report of metabolic signatures and related metabolic pathways in the skeletal muscle for miR-499-5p injection and provides new insight into the effect of miRNA on growth performance.</p>https://aab.copernicus.org/articles/65/275/2022/aab-65-275-2022.pdf |
| spellingShingle | C. Chen C. Chen J. Li J. Li Z. Li Z. Li Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens Archives Animal Breeding |
| title | Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens |
| title_full | Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens |
| title_fullStr | Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens |
| title_full_unstemmed | Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens |
| title_short | Sustained injection of miR-499-5p alters the gastrocnemius muscle metabolome in broiler chickens |
| title_sort | sustained injection of mir 499 5p alters the gastrocnemius muscle metabolome in broiler chickens |
| url | https://aab.copernicus.org/articles/65/275/2022/aab-65-275-2022.pdf |
| work_keys_str_mv | AT cchen sustainedinjectionofmir4995paltersthegastrocnemiusmusclemetabolomeinbroilerchickens AT cchen sustainedinjectionofmir4995paltersthegastrocnemiusmusclemetabolomeinbroilerchickens AT jli sustainedinjectionofmir4995paltersthegastrocnemiusmusclemetabolomeinbroilerchickens AT jli sustainedinjectionofmir4995paltersthegastrocnemiusmusclemetabolomeinbroilerchickens AT zli sustainedinjectionofmir4995paltersthegastrocnemiusmusclemetabolomeinbroilerchickens AT zli sustainedinjectionofmir4995paltersthegastrocnemiusmusclemetabolomeinbroilerchickens |