In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods
<em>Candida</em> bloodstream infections are a significant cause of morbidity and mortality in hospitalized patients. The most important contribution of biofilm is the higher antifungal resistance than planktonic cells. We aimed to investigate the biofilm formation rate and antifungal sus...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-09-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/mr/article/view/6916 |
| _version_ | 1798031515135770624 |
|---|---|
| author | Yasemin Oz Iman Qoraan Egemen Gokbolat |
| author_facet | Yasemin Oz Iman Qoraan Egemen Gokbolat |
| author_sort | Yasemin Oz |
| collection | DOAJ |
| description | <em>Candida</em> bloodstream infections are a significant cause of morbidity and mortality in hospitalized patients. The most important contribution of biofilm is the higher antifungal resistance than planktonic cells. We aimed to investigate the biofilm formation rate and antifungal susceptibility characteristics of our bloodstream isolates, and evaluate two different biofilm detection methods. A total of 200 bloodstream <em>Candida</em> isolates were included. The biofilms were formed on 96-well microtiter plates and measured by spectrophotometric percent transmittance and 2,3-bis(2- methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium- 5-carboxanilide colorimetric assay. In addition antifungal susceptibilities of these isolates were evaluated against caspofungin, anidulafungin and amphotericin B by reference method. Biofilm production rate was considerably high among our bloodstream isolates. The most important biofilm producer species was <em>C. tropicalis;</em> <em>C. glabrata</em> had the lowest biofilm production rate. The consistency rate between biofilm detection methods was 66%. Remarkable antifungal resistance was not observed among our isolates in general. In conclusion, biofilm production in <em>Candida</em> species is an important virulence factor, and its rate is considerably high in bloodstream isolates. At present, a standardized method has not been established to detect the biofilm formation. |
| first_indexed | 2024-04-11T19:58:42Z |
| format | Article |
| id | doaj.art-04c6c44af18a4fb7955aa35643e501d6 |
| institution | Directory Open Access Journal |
| issn | 2036-7473 2036-7481 |
| language | English |
| last_indexed | 2024-04-11T19:58:42Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-04c6c44af18a4fb7955aa35643e501d62022-12-22T04:05:46ZengMDPI AGMicrobiology Research2036-74732036-74812017-09-018110.4081/mr.2017.69163773In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methodsYasemin Oz0Iman Qoraan1Egemen Gokbolat2Department of Microbiology, Faculty of Medicine, Eskisehir Osmangazi University, EskisehirDepartment of Microbiology, Faculty of Medicine, Eskisehir Osmangazi University, EskisehirDepartment of Microbiology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir<em>Candida</em> bloodstream infections are a significant cause of morbidity and mortality in hospitalized patients. The most important contribution of biofilm is the higher antifungal resistance than planktonic cells. We aimed to investigate the biofilm formation rate and antifungal susceptibility characteristics of our bloodstream isolates, and evaluate two different biofilm detection methods. A total of 200 bloodstream <em>Candida</em> isolates were included. The biofilms were formed on 96-well microtiter plates and measured by spectrophotometric percent transmittance and 2,3-bis(2- methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium- 5-carboxanilide colorimetric assay. In addition antifungal susceptibilities of these isolates were evaluated against caspofungin, anidulafungin and amphotericin B by reference method. Biofilm production rate was considerably high among our bloodstream isolates. The most important biofilm producer species was <em>C. tropicalis;</em> <em>C. glabrata</em> had the lowest biofilm production rate. The consistency rate between biofilm detection methods was 66%. Remarkable antifungal resistance was not observed among our isolates in general. In conclusion, biofilm production in <em>Candida</em> species is an important virulence factor, and its rate is considerably high in bloodstream isolates. At present, a standardized method has not been established to detect the biofilm formation.http://www.pagepress.org/journals/index.php/mr/article/view/6916antifungal susceptibility, biofilm formation, bloodstream, Candida, XTT |
| spellingShingle | Yasemin Oz Iman Qoraan Egemen Gokbolat In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods Microbiology Research antifungal susceptibility, biofilm formation, bloodstream, Candida, XTT |
| title | In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods |
| title_full | In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods |
| title_fullStr | In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods |
| title_full_unstemmed | In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods |
| title_short | In vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods |
| title_sort | in vitro antifungal susceptibility of candidemia agents and detection of their biofilm production by two different methods |
| topic | antifungal susceptibility, biofilm formation, bloodstream, Candida, XTT |
| url | http://www.pagepress.org/journals/index.php/mr/article/view/6916 |
| work_keys_str_mv | AT yaseminoz invitroantifungalsusceptibilityofcandidemiaagentsanddetectionoftheirbiofilmproductionbytwodifferentmethods AT imanqoraan invitroantifungalsusceptibilityofcandidemiaagentsanddetectionoftheirbiofilmproductionbytwodifferentmethods AT egemengokbolat invitroantifungalsusceptibilityofcandidemiaagentsanddetectionoftheirbiofilmproductionbytwodifferentmethods |