Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China
Abstract: China is the largest potato producing country worldwide, with this crop representing the fourth largest staple food crop in China. However, the steady presence of Potato spindle tuber viroid (PSTVd) over the past five decades has a significant economic impact on potato production. To deter...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-02-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915611753 |
| _version_ | 1818739000058314752 |
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| author | Cai-ling QIU Zhi-xiang ZHANG Shi-fang LI Yan-ju BAI Shang-wu LIU Guo-quan FAN Yan-ling GAO Wei ZHANG Shu ZHANG Wen-he LÜ Dian-qiu LÜ |
| author_facet | Cai-ling QIU Zhi-xiang ZHANG Shi-fang LI Yan-ju BAI Shang-wu LIU Guo-quan FAN Yan-ling GAO Wei ZHANG Shu ZHANG Wen-he LÜ Dian-qiu LÜ |
| author_sort | Cai-ling QIU |
| collection | DOAJ |
| description | Abstract: China is the largest potato producing country worldwide, with this crop representing the fourth largest staple food crop in China. However, the steady presence of Potato spindle tuber viroid (PSTVd) over the past five decades has a significant economic impact on potato production. To determine why PSTVd control measures have been ineffective in China, more than 1 000 seed potatoes collected between 2009 and 2014 were subjected to PSTVd detection at the Supervision and Testing Center for Virus-free Seed Potatoes Quality, Ministry of Agriculture, China. A high PSTVd infection rate (6.5%) was detected among these commercial seed potatoes. Some breeding lines of potato collected from 2012 to 2015 were also tested for PSTVd infection, revealing a high rate of PSTVd contamination in these potato propagation materials. Furthermore, comparison of the full-length sequences of 71 different Chinese PSTVd isolates revealed a total of 74 predominant PSTVd variants, which represented 42 different sequence variants of PSTVd. Comparative sequence analysis revealed 30 novel PSTVd sequence variants specific to China. Comprehensive phylogenetic analysis uncovered a close relationship between the Chinese PSTVd sequence variants and those isolated from Russia. It is worth noting that three intermediate strains and six mild strains were identified among these variants. These results have important implications for explaining the ineffective control of PSTVd in China and thus could serve as a basic reference for designing more effective measures to eliminate PSTVd from China in the future. |
| first_indexed | 2024-12-18T01:17:52Z |
| format | Article |
| id | doaj.art-04d53bdd87d04a7b9774bd284bbd6b3d |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T01:17:52Z |
| publishDate | 2016-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-04d53bdd87d04a7b9774bd284bbd6b3d2022-12-21T21:25:54ZengElsevierJournal of Integrative Agriculture2095-31192016-02-01152349363Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North ChinaCai-ling QIU0Zhi-xiang ZHANG1Shi-fang LI2Yan-ju BAI3Shang-wu LIU4Guo-quan FAN5Yan-ling GAO6Wei ZHANG7Shu ZHANG8Wen-he LÜ9Dian-qiu LÜ10Northeast Agricultural University, Harbin 150030, P.R.China; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China; QIU Cai-lingState Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaNortheast Agricultural University, Harbin 150030, P.R.China; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.ChinaVirus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.ChinaVirus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.ChinaNortheast Agricultural University, Harbin 150030, P.R.China; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.ChinaNortheast Agricultural University, Harbin 150030, P.R.China; Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.ChinaVirus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.ChinaNortheast Agricultural University, Harbin 150030, P.R.China; Correspondence LÜ Wen-he, Tel: +86-451-55191763Virus-free Seedling Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China; LÜ Dian-qiu, Tel/Fax: +86-451-86619234Abstract: China is the largest potato producing country worldwide, with this crop representing the fourth largest staple food crop in China. However, the steady presence of Potato spindle tuber viroid (PSTVd) over the past five decades has a significant economic impact on potato production. To determine why PSTVd control measures have been ineffective in China, more than 1 000 seed potatoes collected between 2009 and 2014 were subjected to PSTVd detection at the Supervision and Testing Center for Virus-free Seed Potatoes Quality, Ministry of Agriculture, China. A high PSTVd infection rate (6.5%) was detected among these commercial seed potatoes. Some breeding lines of potato collected from 2012 to 2015 were also tested for PSTVd infection, revealing a high rate of PSTVd contamination in these potato propagation materials. Furthermore, comparison of the full-length sequences of 71 different Chinese PSTVd isolates revealed a total of 74 predominant PSTVd variants, which represented 42 different sequence variants of PSTVd. Comparative sequence analysis revealed 30 novel PSTVd sequence variants specific to China. Comprehensive phylogenetic analysis uncovered a close relationship between the Chinese PSTVd sequence variants and those isolated from Russia. It is worth noting that three intermediate strains and six mild strains were identified among these variants. These results have important implications for explaining the ineffective control of PSTVd in China and thus could serve as a basic reference for designing more effective measures to eliminate PSTVd from China in the future.http://www.sciencedirect.com/science/article/pii/S2095311915611753certificationgermplasmphylogenetic analysisquarantineseed potatoviroid |
| spellingShingle | Cai-ling QIU Zhi-xiang ZHANG Shi-fang LI Yan-ju BAI Shang-wu LIU Guo-quan FAN Yan-ling GAO Wei ZHANG Shu ZHANG Wen-he LÜ Dian-qiu LÜ Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China Journal of Integrative Agriculture certification germplasm phylogenetic analysis quarantine seed potato viroid |
| title | Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China |
| title_full | Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China |
| title_fullStr | Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China |
| title_full_unstemmed | Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China |
| title_short | Occurrence and molecular characterization of Potato spindle tuber viroid (PSTVd) isolates from potato plants in North China |
| title_sort | occurrence and molecular characterization of potato spindle tuber viroid pstvd isolates from potato plants in north china |
| topic | certification germplasm phylogenetic analysis quarantine seed potato viroid |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915611753 |
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