Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows
Improving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 (<i>ACSL1</i>) is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactatio...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/10/12/2282 |
| _version_ | 1827700802237497344 |
|---|---|
| author | Yan Liang Qisong Gao Qiang Zhang Abdelaziz Adam Idriss Arbab Mingxun Li Zhangping Yang Niel A. Karrow Yongjiang Mao |
| author_facet | Yan Liang Qisong Gao Qiang Zhang Abdelaziz Adam Idriss Arbab Mingxun Li Zhangping Yang Niel A. Karrow Yongjiang Mao |
| author_sort | Yan Liang |
| collection | DOAJ |
| description | Improving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 (<i>ACSL1</i>) is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactation performance of cows, including fat percentage, milk protein percentage etc. Our study was aimed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in <i>ACSL1</i> on milk production traits. Twenty Chinese Holstein cows were randomly selected to extract DNA from their blood samples for PCR amplification and sequencing to identify SNPs of the bovine <i>ACSL1</i> gene, and six SNPs (5’UTR-g.20523C>G, g.35446C>T, g.35651G>A, g.35827C>T, g.35941G>A and g.51472C>T) were discovered. Then, Holstein cow genotyping (n = 992) was performed by Sequenom MassARRAY based on former SNP information. Associations between SNPs and milk production traits and somatic cell score (SCS) were analyzed by the least-squares method. The results showed that SNP g.35827C>T was in high linkage disequilibrium with g.35941G>A. Significant associations were found between SNPs and test-day milk yield (TDMY), fat content (FC), protein content (PC) and SCS (<i>p</i> < 0.05). Among these SNPs, SNP 5’UTR-g.20523C>G showed an extremely significant effect on PC and SCS (<i>p</i> < 0.01). The SNP g.35446C>T showed a statistically significant effect on FC, PC, and SCS (<i>p</i> < 0.01), and also TDMY (<i>p</i> < 0.05). The SNP g.35651G>A had a statistically significant effect on PC (<i>p</i> < 0.01). The SNP g.35827C>T showed a highly significant effect on TDMY, FC, and SCS (<i>p</i> < 0.01) and significantly influenced PC (<i>p</i> < 0.05). Lastly, SNP g.51472C>T was significantly associated with TDMY, FC, and SCS (<i>p</i> < 0.05). In summary, the pleiotropic effects of bovine <i>ACSL1</i> for milk production traits were found in this paper, but further investigation will be required on the intrinsic correlation to provide a theoretical basis for the research on molecular genetics of milk quality traits of Holstein cows. |
| first_indexed | 2024-03-10T14:21:01Z |
| format | Article |
| id | doaj.art-04d7016896644ba78d7c8a8861b5ac92 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:21:01Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-04d7016896644ba78d7c8a8861b5ac922023-11-20T23:22:26ZengMDPI AGAnimals2076-26152020-12-011012228210.3390/ani10122282Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein CowsYan Liang0Qisong Gao1Qiang Zhang2Abdelaziz Adam Idriss Arbab3Mingxun Li4Zhangping Yang5Niel A. Karrow6Yongjiang Mao7Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCenter for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, CanadaKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaImproving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 (<i>ACSL1</i>) is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactation performance of cows, including fat percentage, milk protein percentage etc. Our study was aimed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in <i>ACSL1</i> on milk production traits. Twenty Chinese Holstein cows were randomly selected to extract DNA from their blood samples for PCR amplification and sequencing to identify SNPs of the bovine <i>ACSL1</i> gene, and six SNPs (5’UTR-g.20523C>G, g.35446C>T, g.35651G>A, g.35827C>T, g.35941G>A and g.51472C>T) were discovered. Then, Holstein cow genotyping (n = 992) was performed by Sequenom MassARRAY based on former SNP information. Associations between SNPs and milk production traits and somatic cell score (SCS) were analyzed by the least-squares method. The results showed that SNP g.35827C>T was in high linkage disequilibrium with g.35941G>A. Significant associations were found between SNPs and test-day milk yield (TDMY), fat content (FC), protein content (PC) and SCS (<i>p</i> < 0.05). Among these SNPs, SNP 5’UTR-g.20523C>G showed an extremely significant effect on PC and SCS (<i>p</i> < 0.01). The SNP g.35446C>T showed a statistically significant effect on FC, PC, and SCS (<i>p</i> < 0.01), and also TDMY (<i>p</i> < 0.05). The SNP g.35651G>A had a statistically significant effect on PC (<i>p</i> < 0.01). The SNP g.35827C>T showed a highly significant effect on TDMY, FC, and SCS (<i>p</i> < 0.01) and significantly influenced PC (<i>p</i> < 0.05). Lastly, SNP g.51472C>T was significantly associated with TDMY, FC, and SCS (<i>p</i> < 0.05). In summary, the pleiotropic effects of bovine <i>ACSL1</i> for milk production traits were found in this paper, but further investigation will be required on the intrinsic correlation to provide a theoretical basis for the research on molecular genetics of milk quality traits of Holstein cows.https://www.mdpi.com/2076-2615/10/12/2282Holstein cowsSNPs<i>ACSL1</i>milk production traits |
| spellingShingle | Yan Liang Qisong Gao Qiang Zhang Abdelaziz Adam Idriss Arbab Mingxun Li Zhangping Yang Niel A. Karrow Yongjiang Mao Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows Animals Holstein cows SNPs <i>ACSL1</i> milk production traits |
| title | Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows |
| title_full | Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows |
| title_fullStr | Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows |
| title_full_unstemmed | Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows |
| title_short | Polymorphisms of the <i>ACSL1</i> Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows |
| title_sort | polymorphisms of the i acsl1 i gene influence milk production traits and somatic cell score in chinese holstein cows |
| topic | Holstein cows SNPs <i>ACSL1</i> milk production traits |
| url | https://www.mdpi.com/2076-2615/10/12/2282 |
| work_keys_str_mv | AT yanliang polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows AT qisonggao polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows AT qiangzhang polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows AT abdelazizadamidrissarbab polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows AT mingxunli polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows AT zhangpingyang polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows AT nielakarrow polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows AT yongjiangmao polymorphismsoftheiacsl1igeneinfluencemilkproductiontraitsandsomaticcellscoreinchineseholsteincows |