Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats
ABSTRACT Gastrointestinal microorganisms play a crucial role in host survival and adaptation, but information on host-specific selection or environmental factors that shape the microbiome in natural populations is limited. In this study, we employed 16S rRNA gene amplicon sequencing to investigate t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2023-12-01
|
| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02073-23 |
| _version_ | 1797393574704185344 |
|---|---|
| author | Yue Dong Yixuan Li Meiling Ge Tetsuya Takatsu Zongling Wang Xuelei Zhang Dewen Ding Qinzeng Xu |
| author_facet | Yue Dong Yixuan Li Meiling Ge Tetsuya Takatsu Zongling Wang Xuelei Zhang Dewen Ding Qinzeng Xu |
| author_sort | Yue Dong |
| collection | DOAJ |
| description | ABSTRACT Gastrointestinal microorganisms play a crucial role in host survival and adaptation, but information on host-specific selection or environmental factors that shape the microbiome in natural populations is limited. In this study, we employed 16S rRNA gene amplicon sequencing to investigate the composition and predicted the functions of gut microbiota of two ophiuroid species (Ophiura sarsii and its subspecies O. sarsii vadicola) from cold-water habitats across three geographically distant sea areas in the Northern Pacific Ocean. We also explored the potential influence of the host and environment on the microbiota. The two ophiuroids possessed diverse microbial communities, with Proteobacteria being the most dominant phylum in all four groups. Aliivibrio was the predominant genus in O. sarsii from the Bering Sea, while Candidatus Hepatoplasma was the dominant genus in O. sarsii from the Japan Sea and O. sarsii vadicola from the Yellow Sea. Predicted bacterial functions indicated that most of the pathways with significant differences belonged to metabolism and genetic information processing. Notably, no significant difference was observed between the two coexisting ophiuroid species in the Japan Sea. The high similarity in microbial communities between O. sarsii from the Japan Sea and O. sarsii vadicola from the Yellow Sea may be attributed to their analogous ecological niche. The host and the environment jointly shape the composition of the gut microbial community. The presence of specific microorganisms supports the ecological success of ophiuroids across diverse environments, providing a foundation for host adaptation to cold-water habitats. IMPORTANCE Gastrointestinal microorganisms are critical to the survival and adaptation of hosts, and there are few studies on the differences and functions of gastrointestinal microbes in widely distributed species. This study investigated the gut microbes of two ophiuroid species (Ophiura sarsii and its subspecies O. sarsii vadicola) in cold-water habitats of the Northern Pacific Ocean. The results showed that a combination of host and environmental factors shapes the intestinal microbiota of ophiuroids. There was a high similarity in microbial communities between the two groups living in different regions, which may be related to their similar ecological niches. These microorganisms played a vital role in the ecological success of ophiuroids as the foundation for their adaptation to cold-water environments. This study revealed the complex relationship between hosts and their gut microbes, providing insights into the role they play in the adaptation and survival of marine species. |
| first_indexed | 2024-03-09T00:04:40Z |
| format | Article |
| id | doaj.art-04d7c20da1f5459686854b0bddb14d8f |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-09T00:04:40Z |
| publishDate | 2023-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-04d7c20da1f5459686854b0bddb14d8f2023-12-12T13:17:21ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-12-0111610.1128/spectrum.02073-23Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitatsYue Dong0Yixuan Li1Meiling Ge2Tetsuya Takatsu3Zongling Wang4Xuelei Zhang5Dewen Ding6Qinzeng Xu7College of Environmental Science and Engineering, Ocean University of China , Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources , Qingdao, ChinaCollege of Environmental Science and Engineering, Ocean University of China , Qingdao, ChinaFaculty of Fisheries Sciences, Hokkaido University , Hakodate, JapanKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources , Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources , Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources , Qingdao, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources , Qingdao, ChinaABSTRACT Gastrointestinal microorganisms play a crucial role in host survival and adaptation, but information on host-specific selection or environmental factors that shape the microbiome in natural populations is limited. In this study, we employed 16S rRNA gene amplicon sequencing to investigate the composition and predicted the functions of gut microbiota of two ophiuroid species (Ophiura sarsii and its subspecies O. sarsii vadicola) from cold-water habitats across three geographically distant sea areas in the Northern Pacific Ocean. We also explored the potential influence of the host and environment on the microbiota. The two ophiuroids possessed diverse microbial communities, with Proteobacteria being the most dominant phylum in all four groups. Aliivibrio was the predominant genus in O. sarsii from the Bering Sea, while Candidatus Hepatoplasma was the dominant genus in O. sarsii from the Japan Sea and O. sarsii vadicola from the Yellow Sea. Predicted bacterial functions indicated that most of the pathways with significant differences belonged to metabolism and genetic information processing. Notably, no significant difference was observed between the two coexisting ophiuroid species in the Japan Sea. The high similarity in microbial communities between O. sarsii from the Japan Sea and O. sarsii vadicola from the Yellow Sea may be attributed to their analogous ecological niche. The host and the environment jointly shape the composition of the gut microbial community. The presence of specific microorganisms supports the ecological success of ophiuroids across diverse environments, providing a foundation for host adaptation to cold-water habitats. IMPORTANCE Gastrointestinal microorganisms are critical to the survival and adaptation of hosts, and there are few studies on the differences and functions of gastrointestinal microbes in widely distributed species. This study investigated the gut microbes of two ophiuroid species (Ophiura sarsii and its subspecies O. sarsii vadicola) in cold-water habitats of the Northern Pacific Ocean. The results showed that a combination of host and environmental factors shapes the intestinal microbiota of ophiuroids. There was a high similarity in microbial communities between the two groups living in different regions, which may be related to their similar ecological niches. These microorganisms played a vital role in the ecological success of ophiuroids as the foundation for their adaptation to cold-water environments. This study revealed the complex relationship between hosts and their gut microbes, providing insights into the role they play in the adaptation and survival of marine species.https://journals.asm.org/doi/10.1128/spectrum.02073-23cold-water environmentecological nichegut microbiotahost differentiationOphiuroidea |
| spellingShingle | Yue Dong Yixuan Li Meiling Ge Tetsuya Takatsu Zongling Wang Xuelei Zhang Dewen Ding Qinzeng Xu Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats Microbiology Spectrum cold-water environment ecological niche gut microbiota host differentiation Ophiuroidea |
| title | Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats |
| title_full | Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats |
| title_fullStr | Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats |
| title_full_unstemmed | Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats |
| title_short | Distinct gut microbial communities and functional predictions in divergent ophiuroid species: host differentiation, ecological niches, and adaptation to cold-water habitats |
| title_sort | distinct gut microbial communities and functional predictions in divergent ophiuroid species host differentiation ecological niches and adaptation to cold water habitats |
| topic | cold-water environment ecological niche gut microbiota host differentiation Ophiuroidea |
| url | https://journals.asm.org/doi/10.1128/spectrum.02073-23 |
| work_keys_str_mv | AT yuedong distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats AT yixuanli distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats AT meilingge distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats AT tetsuyatakatsu distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats AT zonglingwang distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats AT xueleizhang distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats AT dewending distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats AT qinzengxu distinctgutmicrobialcommunitiesandfunctionalpredictionsindivergentophiuroidspecieshostdifferentiationecologicalnichesandadaptationtocoldwaterhabitats |