Effect of thimerosal on thyroid hormones metabolism in rats
Mercury seems to exert an inhibitory effect on deiodinases, but there are few studies using Thimerosal (TM) as the mercury source. We aimed to elucidate the effect of TM on thyroid hormones peripheral metabolism. Adult Wistar female rats received 0.25 μg or 250 μg TM/100 g BW, IM, twice a week, for...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Bioscientifica
2017-11-01
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| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | http://www.endocrineconnections.com/content/6/8/741.full |
| _version_ | 1828155446135881728 |
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| author | Thiago U Pantaleão Andrea C F Ferreira Maria C S Santos Álvaro S P Figueiredo Ruy A N Louzada Doris Rosenthal Denise P Carvalho Vânia M Corrêa da Costa |
| author_facet | Thiago U Pantaleão Andrea C F Ferreira Maria C S Santos Álvaro S P Figueiredo Ruy A N Louzada Doris Rosenthal Denise P Carvalho Vânia M Corrêa da Costa |
| author_sort | Thiago U Pantaleão |
| collection | DOAJ |
| description | Mercury seems to exert an inhibitory effect on deiodinases, but there are few studies using Thimerosal (TM) as the mercury source. We aimed to elucidate the effect of TM on thyroid hormones peripheral metabolism. Adult Wistar female rats received 0.25 μg or 250 μg TM/100 g BW, IM, twice a week, for a month. We evaluated serum total T3 and T4, D1 activity using 125I-rT3 as tracer, and D2 activity using 125I-T4. NADPH oxidase activity was measured by Amplex-red/HRP method and mRNA levels by real time PCR. Serum T4 was increased and T3 decreased by the greatest dose of TM. Even though D1 activity in pituitary and kidney was reduced by the highest dose of TM, hepatic D1 activity and D1 mRNA levels remained unchanged. D2 activity was also significantly decreased by the highest dose of TM in all CNS samples tested, except cerebellum, but D2 mRNA was unaltered. mRNA levels of the tested NADPH oxidases were not affected by TM and NADPH oxidase activity was either unaltered or decreased. Our results indicate that TM might directly interact with deiodinases, inhibiting their activity probably by binding to their selenium catalytic site, without changes in enzyme expression. |
| first_indexed | 2024-04-11T22:54:06Z |
| format | Article |
| id | doaj.art-04dc1c37c76349c2914eff9dc1d3ed51 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 2049-3614 |
| language | English |
| last_indexed | 2024-04-11T22:54:06Z |
| publishDate | 2017-11-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-04dc1c37c76349c2914eff9dc1d3ed512022-12-22T03:58:29ZengBioscientificaEndocrine Connections2049-36142049-36142017-11-016874174710.1530/EC-17-0220Effect of thimerosal on thyroid hormones metabolism in ratsThiago U Pantaleão0Andrea C F Ferreira1Maria C S Santos2Álvaro S P Figueiredo3Ruy A N Louzada4Doris Rosenthal5Denise P Carvalho6Vânia M Corrêa da Costa7Laboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; NUMPEX, Pólo de Xerém, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Fisiologia Endócrina Doris Rosenthal, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilMercury seems to exert an inhibitory effect on deiodinases, but there are few studies using Thimerosal (TM) as the mercury source. We aimed to elucidate the effect of TM on thyroid hormones peripheral metabolism. Adult Wistar female rats received 0.25 μg or 250 μg TM/100 g BW, IM, twice a week, for a month. We evaluated serum total T3 and T4, D1 activity using 125I-rT3 as tracer, and D2 activity using 125I-T4. NADPH oxidase activity was measured by Amplex-red/HRP method and mRNA levels by real time PCR. Serum T4 was increased and T3 decreased by the greatest dose of TM. Even though D1 activity in pituitary and kidney was reduced by the highest dose of TM, hepatic D1 activity and D1 mRNA levels remained unchanged. D2 activity was also significantly decreased by the highest dose of TM in all CNS samples tested, except cerebellum, but D2 mRNA was unaltered. mRNA levels of the tested NADPH oxidases were not affected by TM and NADPH oxidase activity was either unaltered or decreased. Our results indicate that TM might directly interact with deiodinases, inhibiting their activity probably by binding to their selenium catalytic site, without changes in enzyme expression.http://www.endocrineconnections.com/content/6/8/741.fullthimerosalthyroid functioniodothyronine deiodinases |
| spellingShingle | Thiago U Pantaleão Andrea C F Ferreira Maria C S Santos Álvaro S P Figueiredo Ruy A N Louzada Doris Rosenthal Denise P Carvalho Vânia M Corrêa da Costa Effect of thimerosal on thyroid hormones metabolism in rats Endocrine Connections thimerosal thyroid function iodothyronine deiodinases |
| title | Effect of thimerosal on thyroid hormones metabolism in rats |
| title_full | Effect of thimerosal on thyroid hormones metabolism in rats |
| title_fullStr | Effect of thimerosal on thyroid hormones metabolism in rats |
| title_full_unstemmed | Effect of thimerosal on thyroid hormones metabolism in rats |
| title_short | Effect of thimerosal on thyroid hormones metabolism in rats |
| title_sort | effect of thimerosal on thyroid hormones metabolism in rats |
| topic | thimerosal thyroid function iodothyronine deiodinases |
| url | http://www.endocrineconnections.com/content/6/8/741.full |
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