Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars.
The methylation of DNA bases plays an important role in numerous biological processes including development, gene expression, and DNA replication. Salmonella is an important foodborne pathogen, and methylation in Salmonella is implicated in virulence. Using single molecule real-time (SMRT) DNA-seque...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4393132?pdf=render |
| _version_ | 1818584488734621696 |
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| author | Cary Pirone-Davies Maria Hoffmann Richard J Roberts Tim Muruvanda Ruth E Timme Errol Strain Yan Luo Justin Payne Khai Luong Yi Song Yu-Chih Tsai Matthew Boitano Tyson A Clark Jonas Korlach Peter S Evans Marc W Allard |
| author_facet | Cary Pirone-Davies Maria Hoffmann Richard J Roberts Tim Muruvanda Ruth E Timme Errol Strain Yan Luo Justin Payne Khai Luong Yi Song Yu-Chih Tsai Matthew Boitano Tyson A Clark Jonas Korlach Peter S Evans Marc W Allard |
| author_sort | Cary Pirone-Davies |
| collection | DOAJ |
| description | The methylation of DNA bases plays an important role in numerous biological processes including development, gene expression, and DNA replication. Salmonella is an important foodborne pathogen, and methylation in Salmonella is implicated in virulence. Using single molecule real-time (SMRT) DNA-sequencing, we sequenced and assembled the complete genomes of eleven Salmonella enterica isolates from nine different serovars, and analysed the whole-genome methylation patterns of each genome. We describe 16 distinct N6-methyladenine (m6A) methylated motifs, one N4-methylcytosine (m4C) motif, and one combined m6A-m4C motif. Eight of these motifs are novel, i.e., they have not been previously described. We also identified the methyltransferases (MTases) associated with 13 of the motifs. Some motifs are conserved across all Salmonella serovars tested, while others were found only in a subset of serovars. Eight of the nine serovars contained a unique methylated motif that was not found in any other serovar (most of these motifs were part of Type I restriction modification systems), indicating the high diversity of methylation patterns present in Salmonella. |
| first_indexed | 2024-12-16T08:21:59Z |
| format | Article |
| id | doaj.art-04dca1afa2eb4085b825d589e0a3e1f3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:21:59Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-04dca1afa2eb4085b825d589e0a3e1f32022-12-21T22:38:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012363910.1371/journal.pone.0123639Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars.Cary Pirone-DaviesMaria HoffmannRichard J RobertsTim MuruvandaRuth E TimmeErrol StrainYan LuoJustin PayneKhai LuongYi SongYu-Chih TsaiMatthew BoitanoTyson A ClarkJonas KorlachPeter S EvansMarc W AllardThe methylation of DNA bases plays an important role in numerous biological processes including development, gene expression, and DNA replication. Salmonella is an important foodborne pathogen, and methylation in Salmonella is implicated in virulence. Using single molecule real-time (SMRT) DNA-sequencing, we sequenced and assembled the complete genomes of eleven Salmonella enterica isolates from nine different serovars, and analysed the whole-genome methylation patterns of each genome. We describe 16 distinct N6-methyladenine (m6A) methylated motifs, one N4-methylcytosine (m4C) motif, and one combined m6A-m4C motif. Eight of these motifs are novel, i.e., they have not been previously described. We also identified the methyltransferases (MTases) associated with 13 of the motifs. Some motifs are conserved across all Salmonella serovars tested, while others were found only in a subset of serovars. Eight of the nine serovars contained a unique methylated motif that was not found in any other serovar (most of these motifs were part of Type I restriction modification systems), indicating the high diversity of methylation patterns present in Salmonella.http://europepmc.org/articles/PMC4393132?pdf=render |
| spellingShingle | Cary Pirone-Davies Maria Hoffmann Richard J Roberts Tim Muruvanda Ruth E Timme Errol Strain Yan Luo Justin Payne Khai Luong Yi Song Yu-Chih Tsai Matthew Boitano Tyson A Clark Jonas Korlach Peter S Evans Marc W Allard Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars. PLoS ONE |
| title | Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars. |
| title_full | Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars. |
| title_fullStr | Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars. |
| title_full_unstemmed | Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars. |
| title_short | Genome-wide methylation patterns in Salmonella enterica Subsp. enterica Serovars. |
| title_sort | genome wide methylation patterns in salmonella enterica subsp enterica serovars |
| url | http://europepmc.org/articles/PMC4393132?pdf=render |
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