Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate
Osteoarthritis (OA) is a degenerative joint condition with limited disease-modifying treatments currently. Palm tocotrienol-rich fraction (TRF) has been previously shown to be effective against OA, but its mechanism of action remains elusive. This study aims to compare serum metabolomic alteration i...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/13/12/2343 |
| _version_ | 1797380236673810432 |
|---|---|
| author | Sophia Ogechi Ekeuku Jen-Kit Tan Hiba Murtadha Al-Saadi Fairus Ahmad Mohd Ramli Elvy Suhana Azlan Mohd Arlamsyah Fadhlullah Zuhair Japar Sidik Juliana Abdul Hamid Soelaiman Ima-Nirwana Kok-Yong Chin |
| author_facet | Sophia Ogechi Ekeuku Jen-Kit Tan Hiba Murtadha Al-Saadi Fairus Ahmad Mohd Ramli Elvy Suhana Azlan Mohd Arlamsyah Fadhlullah Zuhair Japar Sidik Juliana Abdul Hamid Soelaiman Ima-Nirwana Kok-Yong Chin |
| author_sort | Sophia Ogechi Ekeuku |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a degenerative joint condition with limited disease-modifying treatments currently. Palm tocotrienol-rich fraction (TRF) has been previously shown to be effective against OA, but its mechanism of action remains elusive. This study aims to compare serum metabolomic alteration in Sprague–Dawley rats with monosodium iodoacetate (MIA)-induced OA which were treated with palm TRF, glucosamine sulphate, or a combination of both. This study was performed on thirty adult male rats, which were divided into normal control (<i>n</i> = 6) and OA groups (<i>n</i> = 24). The OA group received intra-articular injections of MIA and daily oral treatments of refined olive oil (vehicle, <i>n</i> = 6), palm TRF (100 mg/kg, <i>n</i> = 6), glucosamine sulphate (250 mg/kg, <i>n</i> = 6), or a combination of TRF and glucosamine (<i>n</i> = 6) for four weeks. Serum was collected at the study’s conclusion for metabolomic analysis. The findings revealed that MIA-induced OA influences amino acid metabolism, leading to changes in metabolites associated with the biosynthesis of phenylalanine, tyrosine and tryptophan as well as alterations in the metabolism of phenylalanine, tryptophan, arginine and proline. Supplementation with glucosamine sulphate, TRF, or both effectively reversed these metabolic changes induced by OA. The amelioration of metabolic effects induced by OA is linked to the therapeutic effects of TRF and glucosamine. However, it remains unclear whether these effects are direct or indirect in nature. |
| first_indexed | 2024-03-08T20:35:27Z |
| format | Article |
| id | doaj.art-04def696dcdb4cf6bf61417771e14bae |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T20:35:27Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-04def696dcdb4cf6bf61417771e14bae2023-12-22T14:21:22ZengMDPI AGLife2075-17292023-12-011312234310.3390/life13122343Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine SulphateSophia Ogechi Ekeuku0Jen-Kit Tan1Hiba Murtadha Al-Saadi2Fairus Ahmad3Mohd Ramli Elvy Suhana4Azlan Mohd Arlamsyah5Fadhlullah Zuhair Japar Sidik6Juliana Abdul Hamid7Soelaiman Ima-Nirwana8Kok-Yong Chin9Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras 56000, MalaysiaOsteoarthritis (OA) is a degenerative joint condition with limited disease-modifying treatments currently. Palm tocotrienol-rich fraction (TRF) has been previously shown to be effective against OA, but its mechanism of action remains elusive. This study aims to compare serum metabolomic alteration in Sprague–Dawley rats with monosodium iodoacetate (MIA)-induced OA which were treated with palm TRF, glucosamine sulphate, or a combination of both. This study was performed on thirty adult male rats, which were divided into normal control (<i>n</i> = 6) and OA groups (<i>n</i> = 24). The OA group received intra-articular injections of MIA and daily oral treatments of refined olive oil (vehicle, <i>n</i> = 6), palm TRF (100 mg/kg, <i>n</i> = 6), glucosamine sulphate (250 mg/kg, <i>n</i> = 6), or a combination of TRF and glucosamine (<i>n</i> = 6) for four weeks. Serum was collected at the study’s conclusion for metabolomic analysis. The findings revealed that MIA-induced OA influences amino acid metabolism, leading to changes in metabolites associated with the biosynthesis of phenylalanine, tyrosine and tryptophan as well as alterations in the metabolism of phenylalanine, tryptophan, arginine and proline. Supplementation with glucosamine sulphate, TRF, or both effectively reversed these metabolic changes induced by OA. The amelioration of metabolic effects induced by OA is linked to the therapeutic effects of TRF and glucosamine. However, it remains unclear whether these effects are direct or indirect in nature.https://www.mdpi.com/2075-1729/13/12/2343amino acid metabolismarthritisjointtocotrienolvitamin E |
| spellingShingle | Sophia Ogechi Ekeuku Jen-Kit Tan Hiba Murtadha Al-Saadi Fairus Ahmad Mohd Ramli Elvy Suhana Azlan Mohd Arlamsyah Fadhlullah Zuhair Japar Sidik Juliana Abdul Hamid Soelaiman Ima-Nirwana Kok-Yong Chin Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate Life amino acid metabolism arthritis joint tocotrienol vitamin E |
| title | Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate |
| title_full | Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate |
| title_fullStr | Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate |
| title_full_unstemmed | Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate |
| title_short | Serum Metabolomic Alteration in Rats with Osteoarthritis Treated with Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate |
| title_sort | serum metabolomic alteration in rats with osteoarthritis treated with palm tocotrienol rich fraction alone or in combination with glucosamine sulphate |
| topic | amino acid metabolism arthritis joint tocotrienol vitamin E |
| url | https://www.mdpi.com/2075-1729/13/12/2343 |
| work_keys_str_mv | AT sophiaogechiekeuku serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT jenkittan serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT hibamurtadhaalsaadi serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT fairusahmad serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT mohdramlielvysuhana serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT azlanmohdarlamsyah serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT fadhlullahzuhairjaparsidik serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT julianaabdulhamid serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT soelaimanimanirwana serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate AT kokyongchin serummetabolomicalterationinratswithosteoarthritistreatedwithpalmtocotrienolrichfractionaloneorincombinationwithglucosaminesulphate |