Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis Patients
Rheumatoid arthritis (RA) and osteoarthritis (OA) are destructive joint diseases, the development of which are associated with the expansion of pathogenic T lymphocytes. Mesenchymal stem cells may be an attractive therapeutic option for patients with RA or OA due to the regenerative and immunomodula...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/15/3/1003 |
| _version_ | 1827747977707388928 |
|---|---|
| author | Ewa Kuca-Warnawin Weronika Kurowska Magdalena Plebańczyk Anna Wajda Anna Kornatka Tomasz Burakowski Iwona Janicka Piotr Syrówka Urszula Skalska |
| author_facet | Ewa Kuca-Warnawin Weronika Kurowska Magdalena Plebańczyk Anna Wajda Anna Kornatka Tomasz Burakowski Iwona Janicka Piotr Syrówka Urszula Skalska |
| author_sort | Ewa Kuca-Warnawin |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) and osteoarthritis (OA) are destructive joint diseases, the development of which are associated with the expansion of pathogenic T lymphocytes. Mesenchymal stem cells may be an attractive therapeutic option for patients with RA or OA due to the regenerative and immunomodulatory abilities of these cells. The infrapatellar fat pad (IFP) is a rich and easily available source of mesenchymal stem cells (adipose-derived stem cells, ASCs). However, the phenotypic, potential and immunomodulatory properties of ASCs have not been fully characterised. We aimed to evaluate the phenotype, regenerative potential and effects of IFP-derived ASCs from RA and OA patients on CD4+ T cell proliferation. The MSC phenotype was assessed using flow cytometry. The multipotency of MSCs was evaluated on the basis of their ability to differentiate into adipocytes, chondrocytes and osteoblasts. The immunomodulatory activities of MSCs were examined in co-cultures with sorted CD4+ T cells or peripheral blood mononuclear cells. The concentrations of soluble factors involved in ASC-dependent immunomodulatory activities were assessed in co-culture supernatants using ELISA. We found that ASCs with PPIs from RA and OA patients maintain the ability to differentiate into adipocytes, chondrocytes and osteoblasts. ASCs from RA and OA patients also showed a similar phenotype and comparable abilities to inhibit CD4+ T cell proliferation, which was dependent on the induction of soluble factors The results of our study constitute the basis for further research on the therapeutic potential of ASCs in the treatment of patients with RA and OA. |
| first_indexed | 2024-03-11T06:00:48Z |
| format | Article |
| id | doaj.art-04e16f30111f41bda5069e92ad75f1b6 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T06:00:48Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-04e16f30111f41bda5069e92ad75f1b62023-11-17T13:17:25ZengMDPI AGPharmaceutics1999-49232023-03-01153100310.3390/pharmaceutics15031003Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis PatientsEwa Kuca-Warnawin0Weronika Kurowska1Magdalena Plebańczyk2Anna Wajda3Anna Kornatka4Tomasz Burakowski5Iwona Janicka6Piotr Syrówka7Urszula Skalska8Department of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandDepartment of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandDepartment of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandDepartment of Molecular Biology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandDepartment of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandDepartment of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandDepartment of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandRheumaorthopedics Clinic and Polyclinic, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandDepartment of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, PolandRheumatoid arthritis (RA) and osteoarthritis (OA) are destructive joint diseases, the development of which are associated with the expansion of pathogenic T lymphocytes. Mesenchymal stem cells may be an attractive therapeutic option for patients with RA or OA due to the regenerative and immunomodulatory abilities of these cells. The infrapatellar fat pad (IFP) is a rich and easily available source of mesenchymal stem cells (adipose-derived stem cells, ASCs). However, the phenotypic, potential and immunomodulatory properties of ASCs have not been fully characterised. We aimed to evaluate the phenotype, regenerative potential and effects of IFP-derived ASCs from RA and OA patients on CD4+ T cell proliferation. The MSC phenotype was assessed using flow cytometry. The multipotency of MSCs was evaluated on the basis of their ability to differentiate into adipocytes, chondrocytes and osteoblasts. The immunomodulatory activities of MSCs were examined in co-cultures with sorted CD4+ T cells or peripheral blood mononuclear cells. The concentrations of soluble factors involved in ASC-dependent immunomodulatory activities were assessed in co-culture supernatants using ELISA. We found that ASCs with PPIs from RA and OA patients maintain the ability to differentiate into adipocytes, chondrocytes and osteoblasts. ASCs from RA and OA patients also showed a similar phenotype and comparable abilities to inhibit CD4+ T cell proliferation, which was dependent on the induction of soluble factors The results of our study constitute the basis for further research on the therapeutic potential of ASCs in the treatment of patients with RA and OA.https://www.mdpi.com/1999-4923/15/3/1003adipose-derived stem cellsrheumatoid arthritisosteoarthritistissue regenerationimmunomodulation |
| spellingShingle | Ewa Kuca-Warnawin Weronika Kurowska Magdalena Plebańczyk Anna Wajda Anna Kornatka Tomasz Burakowski Iwona Janicka Piotr Syrówka Urszula Skalska Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis Patients Pharmaceutics adipose-derived stem cells rheumatoid arthritis osteoarthritis tissue regeneration immunomodulation |
| title | Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis Patients |
| title_full | Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis Patients |
| title_fullStr | Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis Patients |
| title_full_unstemmed | Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis Patients |
| title_short | Basic Properties of Adipose-Derived Mesenchymal Stem Cells of Rheumatoid Arthritis and Osteoarthritis Patients |
| title_sort | basic properties of adipose derived mesenchymal stem cells of rheumatoid arthritis and osteoarthritis patients |
| topic | adipose-derived stem cells rheumatoid arthritis osteoarthritis tissue regeneration immunomodulation |
| url | https://www.mdpi.com/1999-4923/15/3/1003 |
| work_keys_str_mv | AT ewakucawarnawin basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT weronikakurowska basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT magdalenaplebanczyk basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT annawajda basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT annakornatka basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT tomaszburakowski basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT iwonajanicka basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT piotrsyrowka basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients AT urszulaskalska basicpropertiesofadiposederivedmesenchymalstemcellsofrheumatoidarthritisandosteoarthritispatients |