Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics
The increase in bacterial resistance to antibiotics is a global problem for public health. In our previous works, it was shown that the application of AgNPs in cow mastitis treatment increased <i>S. aureus</i> and <i>S</i>. <i>dysgalactiae</i> susceptibility to 31...
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/7854 |
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| author | Nina Bogdanchikova Maria Maklakova Luis Jesús Villarreal-Gómez Ekaterina Nefedova Nikolay N. Shkil Evgenii Plotnikov Alexey Pestryakov |
| author_facet | Nina Bogdanchikova Maria Maklakova Luis Jesús Villarreal-Gómez Ekaterina Nefedova Nikolay N. Shkil Evgenii Plotnikov Alexey Pestryakov |
| author_sort | Nina Bogdanchikova |
| collection | DOAJ |
| description | The increase in bacterial resistance to antibiotics is a global problem for public health. In our previous works, it was shown that the application of AgNPs in cow mastitis treatment increased <i>S. aureus</i> and <i>S</i>. <i>dysgalactiae</i> susceptibility to 31 antibiotics due to a decrease in the bacterial efflux effect. The aim of the present work was to shed light on whether the change in adhesive and anti-lysozyme activities caused by AgNPs also contribute to the restoration of bacterial susceptibility to antibiotics. In vivo sampling was performed before and after cow mastitis treatments with antibiotics or AgNPs. The isolates were identified, and the adhesive and anti-lysozyme activities were assessed. These data were compared with the results obtained for in vitro pre-treatment of reference bacteria with AgNPs or antibiotics. The present study revealed that bacterial treatments in vitro and in vivo with AgNPs: (1) decrease the bacterial ability to adhere to cells to start an infection and (2) decrease bacterial anti-lysozyme activity, thereby enhancing the activity of lysozyme, a natural “antibiotic” present in living organisms. The obtained data contribute to the perspective of the future application of AgNPs for recovering the activity of antibiotics rapidly disappearing from the market. |
| first_indexed | 2024-03-11T04:16:46Z |
| format | Article |
| id | doaj.art-04e1a72b825f4517bed183f3beef0e40 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:16:46Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-04e1a72b825f4517bed183f3beef0e402023-11-17T23:01:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01249785410.3390/ijms24097854Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to AntibioticsNina Bogdanchikova0Maria Maklakova1Luis Jesús Villarreal-Gómez2Ekaterina Nefedova3Nikolay N. Shkil4Evgenii Plotnikov5Alexey Pestryakov6Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada 22800, BC, MexicoFacultad de Pedagogía e Innovación Educativa, Universidad Autónoma de Baja California, Av. Monclova Esq con Calle Río Mocorito S/n, Ex-Ejido Coahuila, Mexicali 21360, BC, MexicoFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Blvd. Universitario 1000, Unidad Valle de Las Palmas, Tijuana 22260, BC, MexicoSiberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, 630501 Novosibirsk, RussiaSiberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, 630501 Novosibirsk, RussiaTomsk National Research Medical Center of the Russian Academy of Sciences, Mental Health Research Institute, 634014 Tomsk, RussiaResearch School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, 634050 Tomsk, RussiaThe increase in bacterial resistance to antibiotics is a global problem for public health. In our previous works, it was shown that the application of AgNPs in cow mastitis treatment increased <i>S. aureus</i> and <i>S</i>. <i>dysgalactiae</i> susceptibility to 31 antibiotics due to a decrease in the bacterial efflux effect. The aim of the present work was to shed light on whether the change in adhesive and anti-lysozyme activities caused by AgNPs also contribute to the restoration of bacterial susceptibility to antibiotics. In vivo sampling was performed before and after cow mastitis treatments with antibiotics or AgNPs. The isolates were identified, and the adhesive and anti-lysozyme activities were assessed. These data were compared with the results obtained for in vitro pre-treatment of reference bacteria with AgNPs or antibiotics. The present study revealed that bacterial treatments in vitro and in vivo with AgNPs: (1) decrease the bacterial ability to adhere to cells to start an infection and (2) decrease bacterial anti-lysozyme activity, thereby enhancing the activity of lysozyme, a natural “antibiotic” present in living organisms. The obtained data contribute to the perspective of the future application of AgNPs for recovering the activity of antibiotics rapidly disappearing from the market.https://www.mdpi.com/1422-0067/24/9/7854antibiotics resistanceincrease of bacterial susceptibility to antibioticssilver nanoparticlesanti-lysozyme activityadhesionbovine mastitis |
| spellingShingle | Nina Bogdanchikova Maria Maklakova Luis Jesús Villarreal-Gómez Ekaterina Nefedova Nikolay N. Shkil Evgenii Plotnikov Alexey Pestryakov Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics International Journal of Molecular Sciences antibiotics resistance increase of bacterial susceptibility to antibiotics silver nanoparticles anti-lysozyme activity adhesion bovine mastitis |
| title | Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics |
| title_full | Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics |
| title_fullStr | Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics |
| title_full_unstemmed | Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics |
| title_short | Revealing the Second and the Third Causes of AgNPs Property to Restore the Bacterial Susceptibility to Antibiotics |
| title_sort | revealing the second and the third causes of agnps property to restore the bacterial susceptibility to antibiotics |
| topic | antibiotics resistance increase of bacterial susceptibility to antibiotics silver nanoparticles anti-lysozyme activity adhesion bovine mastitis |
| url | https://www.mdpi.com/1422-0067/24/9/7854 |
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