A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae
Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1999-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.9.825 |
| _version_ | 1818915045557403648 |
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| author | Martin A. Crockard Anthony J. Bjourson James E. Cooper |
| author_facet | Martin A. Crockard Anthony J. Bjourson James E. Cooper |
| author_sort | Martin A. Crockard |
| collection | DOAJ |
| description | Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h. Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen. |
| first_indexed | 2024-12-19T23:56:02Z |
| format | Article |
| id | doaj.art-04e489935cd74ee98f7793f836aec6a6 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:56:02Z |
| publishDate | 1999-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-04e489935cd74ee98f7793f836aec6a62022-12-21T20:01:00ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-09-0112982582810.1094/MPMI.1999.12.9.825A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringaeMartin A. CrockardAnthony J. BjoursonJames E. CooperTemporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h. Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.9.825 |
| spellingShingle | Martin A. Crockard Anthony J. Bjourson James E. Cooper A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae Molecular Plant-Microbe Interactions |
| title | A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae |
| title_full | A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae |
| title_fullStr | A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae |
| title_full_unstemmed | A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae |
| title_short | A New Peroxidase cDNA from White Clover: Its Characterization and Expression in Root Tissue Challenged with Homologous Rhizobia, Heterologous Rhizobia, or Pseudomonas syringae |
| title_sort | new peroxidase cdna from white clover its characterization and expression in root tissue challenged with homologous rhizobia heterologous rhizobia or pseudomonas syringae |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.9.825 |
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