Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light
Maximal and partial quantum yields of photosystem II (Fv/Fm and Fv–/Fp) in Phaseolus vulgaris leaves were determined in response to the turning on saturating and low (undersaturating) actinic light, accordingly. Measuring lights (ML) of blue, green, and red colors were applied simultaneously using a...
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Elsevier
2020-06-01
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| Series: | Information Processing in Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214317319301726 |
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| author | Vladimir Lysenko Ya Guo Alexey Kosolapov Elena Usova Tatyana Varduny Vladimir Krasnov |
| author_facet | Vladimir Lysenko Ya Guo Alexey Kosolapov Elena Usova Tatyana Varduny Vladimir Krasnov |
| author_sort | Vladimir Lysenko |
| collection | DOAJ |
| description | Maximal and partial quantum yields of photosystem II (Fv/Fm and Fv–/Fp) in Phaseolus vulgaris leaves were determined in response to the turning on saturating and low (undersaturating) actinic light, accordingly. Measuring lights (ML) of blue, green, and red colors were applied simultaneously using a novel method of the polychromatic fast Fourier transforming pulse amplitude modulation (FFT PAM) chlorophyll fluorometry. Colors of ML were cross-combined with the colors of low and saturating actinic light. Fv/Fm values measured with the green ML were found to be close to that of red light, whereas application of blue ML leads to lower Fv/Fm. In addition, 5-nm resolved excitation spectra were measured to evaluate the dependence between red/far red fluorescence ratios (peak height based – F685/F730, and deconvolved peak square based – F685sq/F730sq) and wavelength of the excitation light. It demonstrates that chlorophyll fluorescence ratio red/far red may be dependent on the spectral absorbance of chlorophyll. The data obtained confirm the results of the previous studies which explain high photosynthetic activity of the green light in terms of redistribution of absorbed quanta throughout thickness of the leaf, thus suggesting possible advisability of green light application in greenhouse lighting, especially for the plants having thick leaves. |
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| institution | Directory Open Access Journal |
| issn | 2214-3173 |
| language | English |
| last_indexed | 2024-03-12T06:08:17Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
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| series | Information Processing in Agriculture |
| spelling | doaj.art-04e5558c88114c5888662c5804cd02202023-09-03T03:19:21ZengElsevierInformation Processing in Agriculture2214-31732020-06-0172204211Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green lightVladimir Lysenko0Ya Guo1Alexey Kosolapov2Elena Usova3Tatyana Varduny4Vladimir Krasnov5Southern Federal University, Academy of Biology and Biotechnology, Rostov-on-Don, Russia; Russian Information-Analytical and Water Management Research Center, Rostov-on-Don, Russia; Corresponding author at: Southern Federal University, Academy of Biology and Biotechnology, Rostov-on-Don, Russia.School of IoT Engineering, Jiangnan University, Wuxi, ChinaRussian Information-Analytical and Water Management Research Center, Rostov-on-Don, RussiaRussian Information-Analytical and Water Management Research Center, Rostov-on-Don, RussiaSouthern Federal University, Academy of Biology and Biotechnology, Rostov-on-Don, RussiaRussian Information-Analytical and Water Management Research Center, Rostov-on-Don, Russia; Rostov State Medical University, Department of General and Clinical Biochemistry No. 1, Rostov-on-Don, RussiaMaximal and partial quantum yields of photosystem II (Fv/Fm and Fv–/Fp) in Phaseolus vulgaris leaves were determined in response to the turning on saturating and low (undersaturating) actinic light, accordingly. Measuring lights (ML) of blue, green, and red colors were applied simultaneously using a novel method of the polychromatic fast Fourier transforming pulse amplitude modulation (FFT PAM) chlorophyll fluorometry. Colors of ML were cross-combined with the colors of low and saturating actinic light. Fv/Fm values measured with the green ML were found to be close to that of red light, whereas application of blue ML leads to lower Fv/Fm. In addition, 5-nm resolved excitation spectra were measured to evaluate the dependence between red/far red fluorescence ratios (peak height based – F685/F730, and deconvolved peak square based – F685sq/F730sq) and wavelength of the excitation light. It demonstrates that chlorophyll fluorescence ratio red/far red may be dependent on the spectral absorbance of chlorophyll. The data obtained confirm the results of the previous studies which explain high photosynthetic activity of the green light in terms of redistribution of absorbed quanta throughout thickness of the leaf, thus suggesting possible advisability of green light application in greenhouse lighting, especially for the plants having thick leaves.http://www.sciencedirect.com/science/article/pii/S2214317319301726Multicolor PAMFourier transformsChlorophyll fluorescenceGreen lightRed/far red ratioGreenhouse lighting |
| spellingShingle | Vladimir Lysenko Ya Guo Alexey Kosolapov Elena Usova Tatyana Varduny Vladimir Krasnov Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light Information Processing in Agriculture Multicolor PAM Fourier transforms Chlorophyll fluorescence Green light Red/far red ratio Greenhouse lighting |
| title | Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light |
| title_full | Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light |
| title_fullStr | Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light |
| title_full_unstemmed | Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light |
| title_short | Polychromatic Fourier-PAM fluorometry and hyperspectral analysis of chlorophyll fluorescence from Phaseolus vulgaris leaves: Effects of green light |
| title_sort | polychromatic fourier pam fluorometry and hyperspectral analysis of chlorophyll fluorescence from phaseolus vulgaris leaves effects of green light |
| topic | Multicolor PAM Fourier transforms Chlorophyll fluorescence Green light Red/far red ratio Greenhouse lighting |
| url | http://www.sciencedirect.com/science/article/pii/S2214317319301726 |
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