A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats
<p>Abstract</p> <p>Background</p> <p>We have previously shown that either the continuous intake of a palatable hyperlipidic diet (H) or the alternation of chow (C) and an H diet (CH regimen) induced obesity in rats. Here, we investigated whether the time of the start an...
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | Lipids in Health and Disease |
| Online Access: | http://www.lipidworld.com/content/10/1/168 |
| _version_ | 1818563696603955200 |
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| author | Motoyama Caio SM Ribeiro Eliane B Souza Gabriel I Habitante Carlos A Bueno Allain A Oyama Lila M Estadella Debora Oller do Nascimento Claudia M |
| author_facet | Motoyama Caio SM Ribeiro Eliane B Souza Gabriel I Habitante Carlos A Bueno Allain A Oyama Lila M Estadella Debora Oller do Nascimento Claudia M |
| author_sort | Motoyama Caio SM |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>We have previously shown that either the continuous intake of a palatable hyperlipidic diet (H) or the alternation of chow (C) and an H diet (CH regimen) induced obesity in rats. Here, we investigated whether the time of the start and duration of these feeding regimens are relevant and whether they affect brain glucose metabolism.</p> <p>Methods</p> <p>Male Wistar rats received C, H, or CH diets during various periods of their life spans: days 30-60, days 30-90, or days 60-90. Experiments were performed the 60<sup>th </sup>or the 90<sup>th </sup>day of life. Rats were killed by decapitation. The glucose, insulin, leptin plasma concentration, and lipid content of the carcasses were determined. The brain was sliced and incubated with or without insulin for the analysis of glucose uptake, oxidation, and the conversion of [1-<sup>14</sup>C]-glucose to lipids.</p> <p>Results</p> <p>The relative carcass lipid content increased in all of the H and CH groups, and the H30-60 and H30-90 groups had the highest levels. Groups H30-60, H30-90, CH30-60, and CH30-90 exhibited a higher serum glucose level. Serum leptin increased in all H groups and in the CH60-90 and CH30-90 groups. Serum insulin was elevated in the H30-60, H60-90, CH60-90, CH30-90 groups. Basal brain glucose consumption and hypothalamic insulin receptor density were lower only in the CH30-60 group. The rate of brain lipogenesis was increased in the H30-90 and CH30-90 groups.</p> <p>Conclusion</p> <p>These findings indicate that both H and CH diet regimens increased body adiposity independent treatment and the age at which treatment was started, whereas these diets caused hyperglycemia and affected brain metabolism when started at an early age.</p> |
| first_indexed | 2024-12-14T01:19:46Z |
| format | Article |
| id | doaj.art-04ec0c540ca948e5b84409878c54243f |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-14T01:19:46Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-04ec0c540ca948e5b84409878c54243f2022-12-21T23:22:27ZengBMCLipids in Health and Disease1476-511X2011-09-0110116810.1186/1476-511X-10-168A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in ratsMotoyama Caio SMRibeiro Eliane BSouza Gabriel IHabitante Carlos ABueno Allain AOyama Lila MEstadella DeboraOller do Nascimento Claudia M<p>Abstract</p> <p>Background</p> <p>We have previously shown that either the continuous intake of a palatable hyperlipidic diet (H) or the alternation of chow (C) and an H diet (CH regimen) induced obesity in rats. Here, we investigated whether the time of the start and duration of these feeding regimens are relevant and whether they affect brain glucose metabolism.</p> <p>Methods</p> <p>Male Wistar rats received C, H, or CH diets during various periods of their life spans: days 30-60, days 30-90, or days 60-90. Experiments were performed the 60<sup>th </sup>or the 90<sup>th </sup>day of life. Rats were killed by decapitation. The glucose, insulin, leptin plasma concentration, and lipid content of the carcasses were determined. The brain was sliced and incubated with or without insulin for the analysis of glucose uptake, oxidation, and the conversion of [1-<sup>14</sup>C]-glucose to lipids.</p> <p>Results</p> <p>The relative carcass lipid content increased in all of the H and CH groups, and the H30-60 and H30-90 groups had the highest levels. Groups H30-60, H30-90, CH30-60, and CH30-90 exhibited a higher serum glucose level. Serum leptin increased in all H groups and in the CH60-90 and CH30-90 groups. Serum insulin was elevated in the H30-60, H60-90, CH60-90, CH30-90 groups. Basal brain glucose consumption and hypothalamic insulin receptor density were lower only in the CH30-60 group. The rate of brain lipogenesis was increased in the H30-90 and CH30-90 groups.</p> <p>Conclusion</p> <p>These findings indicate that both H and CH diet regimens increased body adiposity independent treatment and the age at which treatment was started, whereas these diets caused hyperglycemia and affected brain metabolism when started at an early age.</p>http://www.lipidworld.com/content/10/1/168 |
| spellingShingle | Motoyama Caio SM Ribeiro Eliane B Souza Gabriel I Habitante Carlos A Bueno Allain A Oyama Lila M Estadella Debora Oller do Nascimento Claudia M A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats Lipids in Health and Disease |
| title | A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats |
| title_full | A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats |
| title_fullStr | A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats |
| title_full_unstemmed | A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats |
| title_short | A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats |
| title_sort | palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats |
| url | http://www.lipidworld.com/content/10/1/168 |
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