Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron Content
The ability of a number of microorganisms isolated from highly magnetic soil of the city Mednogorsk to oxidize Fe (II) under conditions of periodic cultivation in a liquid medium was studied. Among the studied microorganisms, two microbial isolates with maximum growth characteristics and iron-oxidiz...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/13/7/901 |
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| author | Ekaterina Pleshakova Clement Ngun Mikhail Reshetnikov Maxim Viktorovich Larionov |
| author_facet | Ekaterina Pleshakova Clement Ngun Mikhail Reshetnikov Maxim Viktorovich Larionov |
| author_sort | Ekaterina Pleshakova |
| collection | DOAJ |
| description | The ability of a number of microorganisms isolated from highly magnetic soil of the city Mednogorsk to oxidize Fe (II) under conditions of periodic cultivation in a liquid medium was studied. Among the studied microorganisms, two microbial isolates with maximum growth characteristics and iron-oxidizing activity were selected and identified: <i>Bacillus megaterium</i> 69.3 and <i>B. megaterium</i> 69.5. Individual levels of metal resistance of the isolates were determined: maximum tolerated concentration (MTC) for Fe (II) of the isolates <i>B. megaterium</i> 69.3 and <i>B. megaterium</i> 69.5 was 1200 mg L<sup>−1</sup>, minimum inhibitory concentration (MIC) was 1800 mg L<sup>−1</sup>. Both microbial isolates actively oxidized Fe (II) by reducing its high concentration in the medium (1.19 g L<sup>−1</sup>) by 33 and 39% during 14 days of culturing. Total increase in the biomass of <i>B. megaterium</i> 69.3 and <i>B. megaterium</i> 69.5 after 14 days of culturing was 15.3 and 14.7 g L<sup>−1</sup>; the active parts of the biomass increased 8.7- and 6.9-fold compared to the inoculum dose, respectively. These microbial isolates could be used in future in the biotechnological process of water purification with increased/high levels of Fe (II). |
| first_indexed | 2024-03-10T12:52:51Z |
| format | Article |
| id | doaj.art-04ee4181b1ab4e26a9760a5c2b81c99b |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-10T12:52:51Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-04ee4181b1ab4e26a9760a5c2b81c99b2023-11-21T12:06:12ZengMDPI AGWater2073-44412021-03-0113790110.3390/w13070901Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron ContentEkaterina Pleshakova0Clement Ngun1Mikhail Reshetnikov2Maxim Viktorovich Larionov3Department of Biochemistry and Biophysics, Saratov State University, 410012 Saratov, RussiaDepartment of Biochemistry and Biophysics, Saratov State University, 410012 Saratov, RussiaDepartment of Biochemistry and Biophysics, Saratov State University, 410012 Saratov, RussiaFederal State Budgetary Educational Institution of Higher Education, Russian Timiryazev State Agrarian University, 127550 Moscow, RussiaThe ability of a number of microorganisms isolated from highly magnetic soil of the city Mednogorsk to oxidize Fe (II) under conditions of periodic cultivation in a liquid medium was studied. Among the studied microorganisms, two microbial isolates with maximum growth characteristics and iron-oxidizing activity were selected and identified: <i>Bacillus megaterium</i> 69.3 and <i>B. megaterium</i> 69.5. Individual levels of metal resistance of the isolates were determined: maximum tolerated concentration (MTC) for Fe (II) of the isolates <i>B. megaterium</i> 69.3 and <i>B. megaterium</i> 69.5 was 1200 mg L<sup>−1</sup>, minimum inhibitory concentration (MIC) was 1800 mg L<sup>−1</sup>. Both microbial isolates actively oxidized Fe (II) by reducing its high concentration in the medium (1.19 g L<sup>−1</sup>) by 33 and 39% during 14 days of culturing. Total increase in the biomass of <i>B. megaterium</i> 69.3 and <i>B. megaterium</i> 69.5 after 14 days of culturing was 15.3 and 14.7 g L<sup>−1</sup>; the active parts of the biomass increased 8.7- and 6.9-fold compared to the inoculum dose, respectively. These microbial isolates could be used in future in the biotechnological process of water purification with increased/high levels of Fe (II).https://www.mdpi.com/2073-4441/13/7/901ironiron-oxidizing microorganismscultivationbiomass growthwater purification |
| spellingShingle | Ekaterina Pleshakova Clement Ngun Mikhail Reshetnikov Maxim Viktorovich Larionov Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron Content Water iron iron-oxidizing microorganisms cultivation biomass growth water purification |
| title | Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron Content |
| title_full | Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron Content |
| title_fullStr | Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron Content |
| title_full_unstemmed | Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron Content |
| title_short | Evaluation of the Ecological Potential of Microorganisms for Purifying Water with High Iron Content |
| title_sort | evaluation of the ecological potential of microorganisms for purifying water with high iron content |
| topic | iron iron-oxidizing microorganisms cultivation biomass growth water purification |
| url | https://www.mdpi.com/2073-4441/13/7/901 |
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