Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction.
Edwardsiella ictaluri is a Gram-negative facultative anaerobe intracellular bacterium that causes enteric septicemia in channel catfish. Iron is an essential inorganic nutrient of bacteria and is crucial for bacterial invasion. Reduced availability of iron by the host may cause significant stress fo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4500449?pdf=render |
| _version_ | 1811279207474397184 |
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| author | Pradeep R Dumpala Brian C Peterson Mark L Lawrence Attila Karsi |
| author_facet | Pradeep R Dumpala Brian C Peterson Mark L Lawrence Attila Karsi |
| author_sort | Pradeep R Dumpala |
| collection | DOAJ |
| description | Edwardsiella ictaluri is a Gram-negative facultative anaerobe intracellular bacterium that causes enteric septicemia in channel catfish. Iron is an essential inorganic nutrient of bacteria and is crucial for bacterial invasion. Reduced availability of iron by the host may cause significant stress for bacterial pathogens and is considered a signal that leads to significant alteration in virulence gene expression. However, the precise effect of iron-restriction on E. ictaluri protein abundance is unknown. The purpose of this study was to identify differentially abundant proteins of E. ictaluri during in vitro iron-restricted conditions. We applied two-dimensional difference in gel electrophoresis (2D-DIGE) for determining differentially abundant proteins and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF MS) for protein identification. Gene ontology and pathway-based functional modeling of differentially abundant proteins was also conducted. A total of 50 unique differentially abundant proteins at a minimum of 2-fold (p ≤ 0.05) difference in abundance due to iron-restriction were detected. The numbers of up- and down-regulated proteins were 37 and 13, respectively. We noted several proteins, including EsrB, LamB, MalM, MalE, FdaA, and TonB-dependent heme/hemoglobin receptor family proteins responded to iron restriction in E. ictaluri. |
| first_indexed | 2024-04-13T00:50:34Z |
| format | Article |
| id | doaj.art-04efdc0004a6480185394ec885b48a74 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T00:50:34Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-04efdc0004a6480185394ec885b48a742022-12-22T03:09:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01107e013250410.1371/journal.pone.0132504Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction.Pradeep R DumpalaBrian C PetersonMark L LawrenceAttila KarsiEdwardsiella ictaluri is a Gram-negative facultative anaerobe intracellular bacterium that causes enteric septicemia in channel catfish. Iron is an essential inorganic nutrient of bacteria and is crucial for bacterial invasion. Reduced availability of iron by the host may cause significant stress for bacterial pathogens and is considered a signal that leads to significant alteration in virulence gene expression. However, the precise effect of iron-restriction on E. ictaluri protein abundance is unknown. The purpose of this study was to identify differentially abundant proteins of E. ictaluri during in vitro iron-restricted conditions. We applied two-dimensional difference in gel electrophoresis (2D-DIGE) for determining differentially abundant proteins and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF MS) for protein identification. Gene ontology and pathway-based functional modeling of differentially abundant proteins was also conducted. A total of 50 unique differentially abundant proteins at a minimum of 2-fold (p ≤ 0.05) difference in abundance due to iron-restriction were detected. The numbers of up- and down-regulated proteins were 37 and 13, respectively. We noted several proteins, including EsrB, LamB, MalM, MalE, FdaA, and TonB-dependent heme/hemoglobin receptor family proteins responded to iron restriction in E. ictaluri.http://europepmc.org/articles/PMC4500449?pdf=render |
| spellingShingle | Pradeep R Dumpala Brian C Peterson Mark L Lawrence Attila Karsi Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction. PLoS ONE |
| title | Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction. |
| title_full | Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction. |
| title_fullStr | Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction. |
| title_full_unstemmed | Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction. |
| title_short | Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction. |
| title_sort | identification of differentially abundant proteins of edwardsiella ictaluri during iron restriction |
| url | http://europepmc.org/articles/PMC4500449?pdf=render |
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