<i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> Infections
With the practice of a successful livestock industry using antibiotics, which has continued for more than five decades, researchers have long been interested in finding alternatives to antibiotics for poultry production. Probiotics can potentially reduce enteric diseases in livestock and enhance the...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/7/1719 |
| _version_ | 1797588134083428352 |
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| author | Ashraf Khalifa Hairul-Islam Mohamed Ibrahim Abdullah Sheikh |
| author_facet | Ashraf Khalifa Hairul-Islam Mohamed Ibrahim Abdullah Sheikh |
| author_sort | Ashraf Khalifa |
| collection | DOAJ |
| description | With the practice of a successful livestock industry using antibiotics, which has continued for more than five decades, researchers have long been interested in finding alternatives to antibiotics for poultry production. Probiotics can potentially reduce enteric diseases in livestock and enhance their productivity. The aim of this study was to isolate putative probiotics from camel milk and test them against <i>Salmonella</i> infection as well as host immune development. Thirteen different isolates were obtained from six different camel milk samples from dairy farms in Saudi Arabia. Three of the six isolates (PM1, PM2, PM3, PM4, PM5, and PM6) that showed Gram-positive characters reacted negatively to catalase and hemolytic assays. PM1, PM5, and PM6 showed significant nonpolar surface properties (>51% hydrophobic) and potent antimicrobial activities against avian pathogens, namely <i>S. enterica, S. typhi, S. aureus,</i> and <i>E. coli</i>. PM5 exhibited substantial probiotic traits; therefore, further focus was given to it. PM5 was identified as <i>Bacillus subtilis</i> OQ913924 by the 16S rRNA sequencing method and showed similarity matrix > 99%. An in vivo chicken model was used to access the health benefits of probiotics. After <i>salmonella</i> infection, the mucosal immune response was significantly increased (<i>p</i> < 0.01), and none of the challenge protocols caused mortality or clinical symptoms after infection in intestinal contents. <i>S. enterica</i> organ infiltration in the spleen, thymus, and small intestine was significantly reduced in the <i>B. subtilis</i> PM5-fed chickens. The <i>S. enterica</i> load in chicken feces was reduced from CFU 7.2 to 5.2 in oral-fed <i>B. subtilis</i> PM5-fed chickens. Probiotic-fed chickens showed buffered intestinal content and positively regulated the level of butyric acid (<i>p</i> < 0.05), and intestinal interleukin 1 beta (IL1-β), C-reactive protein (CRP), and interferon gamma (IFN-γ) levels were reduced (<i>p</i> < 0.05). In addition, <i>B. subtilis</i> PM5 showed significant binding to peritoneal macrophages cells and inhibited <i>S. enterica</i> surface adhesion, indicating co-aggregation of <i>B. subtilis</i> PM5 in macrophage cells. It could be concluded that supplementation with probiotics can improve the growth performance of broilers and the quality of broiler chickens against enteric pathogens. The introduction of this probiotic into the commercial poultry feed market in the near future may assist in narrowing the gap that now exists between chicken breeding and consumer demand. |
| first_indexed | 2024-03-11T00:48:29Z |
| format | Article |
| id | doaj.art-04effd6fb75b42fdb02268cb069d0270 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T00:48:29Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-04effd6fb75b42fdb02268cb069d02702023-11-18T20:35:29ZengMDPI AGMicroorganisms2076-26072023-06-01117171910.3390/microorganisms11071719<i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> InfectionsAshraf Khalifa0Hairul-Islam Mohamed Ibrahim1Abdullah Sheikh2Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaBiological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaCamel Research Center, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaWith the practice of a successful livestock industry using antibiotics, which has continued for more than five decades, researchers have long been interested in finding alternatives to antibiotics for poultry production. Probiotics can potentially reduce enteric diseases in livestock and enhance their productivity. The aim of this study was to isolate putative probiotics from camel milk and test them against <i>Salmonella</i> infection as well as host immune development. Thirteen different isolates were obtained from six different camel milk samples from dairy farms in Saudi Arabia. Three of the six isolates (PM1, PM2, PM3, PM4, PM5, and PM6) that showed Gram-positive characters reacted negatively to catalase and hemolytic assays. PM1, PM5, and PM6 showed significant nonpolar surface properties (>51% hydrophobic) and potent antimicrobial activities against avian pathogens, namely <i>S. enterica, S. typhi, S. aureus,</i> and <i>E. coli</i>. PM5 exhibited substantial probiotic traits; therefore, further focus was given to it. PM5 was identified as <i>Bacillus subtilis</i> OQ913924 by the 16S rRNA sequencing method and showed similarity matrix > 99%. An in vivo chicken model was used to access the health benefits of probiotics. After <i>salmonella</i> infection, the mucosal immune response was significantly increased (<i>p</i> < 0.01), and none of the challenge protocols caused mortality or clinical symptoms after infection in intestinal contents. <i>S. enterica</i> organ infiltration in the spleen, thymus, and small intestine was significantly reduced in the <i>B. subtilis</i> PM5-fed chickens. The <i>S. enterica</i> load in chicken feces was reduced from CFU 7.2 to 5.2 in oral-fed <i>B. subtilis</i> PM5-fed chickens. Probiotic-fed chickens showed buffered intestinal content and positively regulated the level of butyric acid (<i>p</i> < 0.05), and intestinal interleukin 1 beta (IL1-β), C-reactive protein (CRP), and interferon gamma (IFN-γ) levels were reduced (<i>p</i> < 0.05). In addition, <i>B. subtilis</i> PM5 showed significant binding to peritoneal macrophages cells and inhibited <i>S. enterica</i> surface adhesion, indicating co-aggregation of <i>B. subtilis</i> PM5 in macrophage cells. It could be concluded that supplementation with probiotics can improve the growth performance of broilers and the quality of broiler chickens against enteric pathogens. The introduction of this probiotic into the commercial poultry feed market in the near future may assist in narrowing the gap that now exists between chicken breeding and consumer demand.https://www.mdpi.com/2076-2607/11/7/1719<i>Bacillus</i>camel milkchickeninfectionprobiotics |
| spellingShingle | Ashraf Khalifa Hairul-Islam Mohamed Ibrahim Abdullah Sheikh <i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> Infections Microorganisms <i>Bacillus</i> camel milk chicken infection probiotics |
| title | <i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> Infections |
| title_full | <i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> Infections |
| title_fullStr | <i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> Infections |
| title_full_unstemmed | <i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> Infections |
| title_short | <i>Bacillus subtilis</i> PM5 from Camel Milk Boosts Chicken Immunity and Abrogates <i>Salmonella entertitidis</i> Infections |
| title_sort | i bacillus subtilis i pm5 from camel milk boosts chicken immunity and abrogates i salmonella entertitidis i infections |
| topic | <i>Bacillus</i> camel milk chicken infection probiotics |
| url | https://www.mdpi.com/2076-2607/11/7/1719 |
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