The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved]
RNA-binding protein Fused-in Sarcoma (FUS) plays an essential role in various cellular processes. Mutations in the C-terminal domain region, where the nuclear localization signal (NLS) is located, causes the redistribution of FUS from the nucleus to the cytoplasm. In neurons, neurotoxic aggregates a...
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| Format: | Article |
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F1000 Research Ltd
2023-06-01
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| Online Access: | https://f1000research.com/articles/12-376/v2 |
| _version_ | 1827915911980384256 |
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| author | Peter S. McPherson Carl Laflamme Kathleen Southern Riham Ayoubi Maryam Fotouhi Walaa Alshalfie Zhipeng You |
| author_facet | Peter S. McPherson Carl Laflamme Kathleen Southern Riham Ayoubi Maryam Fotouhi Walaa Alshalfie Zhipeng You |
| author_sort | Peter S. McPherson |
| collection | DOAJ |
| description | RNA-binding protein Fused-in Sarcoma (FUS) plays an essential role in various cellular processes. Mutations in the C-terminal domain region, where the nuclear localization signal (NLS) is located, causes the redistribution of FUS from the nucleus to the cytoplasm. In neurons, neurotoxic aggregates are formed as a result, contributing to neurogenerative diseases. Well-characterized anti-FUS antibodies would enable the reproducibility of FUS research, thereby benefiting the scientific community. In this study, we characterized ten FUS commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs. |
| first_indexed | 2024-03-13T03:06:21Z |
| format | Article |
| id | doaj.art-04f26f0f204a462ab3f9959ffa5b98de |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-03-13T03:06:21Z |
| publishDate | 2023-06-01 |
| publisher | F1000 Research Ltd |
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| series | F1000Research |
| spelling | doaj.art-04f26f0f204a462ab3f9959ffa5b98de2023-06-27T00:00:01ZengF1000 Research LtdF1000Research2046-14022023-06-0112151937The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved]Peter S. McPherson0Carl Laflamme1https://orcid.org/0000-0001-5906-025XKathleen Southern2https://orcid.org/0000-0002-4125-3608Riham Ayoubi3Maryam Fotouhi4Walaa Alshalfie5Zhipeng You6Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, CanadaThe Neuro’s Early Drug Discovery Unit (EDDU), Structural Genomics Consortium, McGill University, Montreal, Québec, H3A 2B4, CanadaRNA-binding protein Fused-in Sarcoma (FUS) plays an essential role in various cellular processes. Mutations in the C-terminal domain region, where the nuclear localization signal (NLS) is located, causes the redistribution of FUS from the nucleus to the cytoplasm. In neurons, neurotoxic aggregates are formed as a result, contributing to neurogenerative diseases. Well-characterized anti-FUS antibodies would enable the reproducibility of FUS research, thereby benefiting the scientific community. In this study, we characterized ten FUS commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.https://f1000research.com/articles/12-376/v2Uniprot ID P35637 FUS RNA-binding protein FUS antibody characterization antibody validation Western Bloteng |
| spellingShingle | Peter S. McPherson Carl Laflamme Kathleen Southern Riham Ayoubi Maryam Fotouhi Walaa Alshalfie Zhipeng You The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] F1000Research Uniprot ID P35637 FUS RNA-binding protein FUS antibody characterization antibody validation Western Blot eng |
| title | The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_full | The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_fullStr | The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_full_unstemmed | The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_short | The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 2 approved] |
| title_sort | identification of high performing antibodies for rna binding protein fus for use in western blot immunoprecipitation and immunofluorescence version 2 peer review 2 approved |
| topic | Uniprot ID P35637 FUS RNA-binding protein FUS antibody characterization antibody validation Western Blot eng |
| url | https://f1000research.com/articles/12-376/v2 |
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