Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020
Objective: to study the bacterial microflora in the sputum of patients with pneumonia caused by SARSCoV-2 or other pathogens.Materials and methods. The bacterial microflora of sputum of 173 patients with pneumonia admitted to hospitals in Khabarovsk and the Khabarovsk Territory in May – June 2020 wa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2020-10-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1362 |
| _version_ | 1797220777490120704 |
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| author | A. P. Bondarenko V. A. Shmylenko O. E. Trotsenko V. O. Kotova L. V. Butakova E. A. Bazykina |
| author_facet | A. P. Bondarenko V. A. Shmylenko O. E. Trotsenko V. O. Kotova L. V. Butakova E. A. Bazykina |
| author_sort | A. P. Bondarenko |
| collection | DOAJ |
| description | Objective: to study the bacterial microflora in the sputum of patients with pneumonia caused by SARSCoV-2 or other pathogens.Materials and methods. The bacterial microflora of sputum of 173 patients with pneumonia admitted to hospitals in Khabarovsk and the Khabarovsk Territory in May – June 2020 was examined. Detection of RNA of the SARS-CoV-2 virus was carried out by PCR with the Vector-PCRRV-2019-nCoV-RG test system (manufactured by the State Scientific Center of VB “Vector”, Koltsovo). Determination of the DNA of mycoplasmosis agents and chlamydia was carried out with the test system “AmpliSens® Mycoplasma pneumoniae/Chlamydophila pneumonia” (manufactured by CRIE). Statistical data processing was performed using the Excel program. Results and discussion. Both groups of patients (Covid-19+ and Covid-19–) had high levels of bacterial flora isolation (81.4 and 74.7 %) including common pathogens for community-acquired pneumonia as well as notable detection frequency of Candida spp. and microbial associations. The group of Covid-19+ patients demonstrated a wider range of detected pathogens, was positive for poly-resistant gram-negative Enterobacteriaceae, non-fermenting gram-negative poly-resistant bacteria, with more expressed manifestation of microbial associations. In the group of Covid-19– participants drug-resistant microflora was presented only by MRSA and MRSE staphylococci. |
| first_indexed | 2024-03-08T13:41:56Z |
| format | Article |
| id | doaj.art-04f48418ac904acd93f6ff6b724036e6 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:54:56Z |
| publishDate | 2020-10-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-04f48418ac904acd93f6ff6b724036e62024-04-05T16:47:15ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2020-10-0103434910.21055/0370-1069-2020-3-43-491220Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020A. P. Bondarenko0V. A. Shmylenko1O. E. Trotsenko2V. O. Kotova3L. V. Butakova4E. A. Bazykina5Khabarovsk Research Institute of Microbiology and EpidemiologyKhabarovsk Research Institute of Microbiology and EpidemiologyKhabarovsk Research Institute of Microbiology and EpidemiologyKhabarovsk Research Institute of Microbiology and EpidemiologyKhabarovsk Research Institute of Microbiology and EpidemiologyKhabarovsk Research Institute of Microbiology and EpidemiologyObjective: to study the bacterial microflora in the sputum of patients with pneumonia caused by SARSCoV-2 or other pathogens.Materials and methods. The bacterial microflora of sputum of 173 patients with pneumonia admitted to hospitals in Khabarovsk and the Khabarovsk Territory in May – June 2020 was examined. Detection of RNA of the SARS-CoV-2 virus was carried out by PCR with the Vector-PCRRV-2019-nCoV-RG test system (manufactured by the State Scientific Center of VB “Vector”, Koltsovo). Determination of the DNA of mycoplasmosis agents and chlamydia was carried out with the test system “AmpliSens® Mycoplasma pneumoniae/Chlamydophila pneumonia” (manufactured by CRIE). Statistical data processing was performed using the Excel program. Results and discussion. Both groups of patients (Covid-19+ and Covid-19–) had high levels of bacterial flora isolation (81.4 and 74.7 %) including common pathogens for community-acquired pneumonia as well as notable detection frequency of Candida spp. and microbial associations. The group of Covid-19+ patients demonstrated a wider range of detected pathogens, was positive for poly-resistant gram-negative Enterobacteriaceae, non-fermenting gram-negative poly-resistant bacteria, with more expressed manifestation of microbial associations. In the group of Covid-19– participants drug-resistant microflora was presented only by MRSA and MRSE staphylococci.https://journal.microbe.ru/jour/article/view/1362community-acquired pneumoniaetiological agentsgroups under observation covid-19+ and covid‑19–sputumbacterial microflora |
| spellingShingle | A. P. Bondarenko V. A. Shmylenko O. E. Trotsenko V. O. Kotova L. V. Butakova E. A. Bazykina Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020 Проблемы особо опасных инфекций community-acquired pneumonia etiological agents groups under observation covid-19+ and covid‑19– sputum bacterial microflora |
| title | Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020 |
| title_full | Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020 |
| title_fullStr | Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020 |
| title_full_unstemmed | Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020 |
| title_short | Characteristics of Bacterial Microflora Isolated from Sputum of Patients with Pneumonia Registered in Khabarovsk City and Khabarovsk Territory in the Initial Period of COVID-19 Pandemic in May–June, 2020 |
| title_sort | characteristics of bacterial microflora isolated from sputum of patients with pneumonia registered in khabarovsk city and khabarovsk territory in the initial period of covid 19 pandemic in may june 2020 |
| topic | community-acquired pneumonia etiological agents groups under observation covid-19+ and covid‑19– sputum bacterial microflora |
| url | https://journal.microbe.ru/jour/article/view/1362 |
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