MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> Biofilms
Quorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as <i>N</i>-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/12/11/1148 |
| _version_ | 1827644120449941504 |
|---|---|
| author | Rattanaburi Pitchapa Sivamoke Dissook Sastia Prama Putri Eiichiro Fukusaki Shuichi Shimma |
| author_facet | Rattanaburi Pitchapa Sivamoke Dissook Sastia Prama Putri Eiichiro Fukusaki Shuichi Shimma |
| author_sort | Rattanaburi Pitchapa |
| collection | DOAJ |
| description | Quorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as <i>N</i>-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on the heterogeneity of QS metabolites in biofilms remains largely unknown. Here, we describe the utilization of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to follow the production of specific metabolites, including QS metabolites, during <i>Pseudomonas putida</i> biofilm development. To do so, a method to grow an agar-based biofilm was first established, and MALDI-MSI was used to detect and visualize the distribution of QS metabolites in biofilms at different cultivation times. This study demonstrated that <i>N</i>-acyl-homoserine lactones are homogeneously produced in the early stages of <i>P. putida</i> biofilm formation. In contrast, the spatial distribution of quinolones and pyochelin correlated with the swarming motility of <i>P. putida</i> in mature biofilms. These two metabolites are involved in the production of extracellular polymeric substances and iron chelators. Our study thus contributes to establishing the specific temporal regulation and spatial distribution of <i>N</i>-acyl-homoserine lactone-related metabolites and quinolone and pyochelin in <i>P. putida</i> biofilms. |
| first_indexed | 2024-03-09T18:08:32Z |
| format | Article |
| id | doaj.art-04fcecebfd254652acff8a437ad1cb93 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T18:08:32Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-04fcecebfd254652acff8a437ad1cb932023-11-24T09:13:50ZengMDPI AGMetabolites2218-19892022-11-011211114810.3390/metabo12111148MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> BiofilmsRattanaburi Pitchapa0Sivamoke Dissook1Sastia Prama Putri2Eiichiro Fukusaki3Shuichi Shimma4Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 5650871, Osaka, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 5650871, Osaka, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 5650871, Osaka, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 5650871, Osaka, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 5650871, Osaka, JapanQuorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as <i>N</i>-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on the heterogeneity of QS metabolites in biofilms remains largely unknown. Here, we describe the utilization of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to follow the production of specific metabolites, including QS metabolites, during <i>Pseudomonas putida</i> biofilm development. To do so, a method to grow an agar-based biofilm was first established, and MALDI-MSI was used to detect and visualize the distribution of QS metabolites in biofilms at different cultivation times. This study demonstrated that <i>N</i>-acyl-homoserine lactones are homogeneously produced in the early stages of <i>P. putida</i> biofilm formation. In contrast, the spatial distribution of quinolones and pyochelin correlated with the swarming motility of <i>P. putida</i> in mature biofilms. These two metabolites are involved in the production of extracellular polymeric substances and iron chelators. Our study thus contributes to establishing the specific temporal regulation and spatial distribution of <i>N</i>-acyl-homoserine lactone-related metabolites and quinolone and pyochelin in <i>P. putida</i> biofilms.https://www.mdpi.com/2218-1989/12/11/1148mass spectrometry imagingquorum sensing<i>Pseudomonas putida</i>biofilmssample preparations |
| spellingShingle | Rattanaburi Pitchapa Sivamoke Dissook Sastia Prama Putri Eiichiro Fukusaki Shuichi Shimma MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> Biofilms Metabolites mass spectrometry imaging quorum sensing <i>Pseudomonas putida</i> biofilms sample preparations |
| title | MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> Biofilms |
| title_full | MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> Biofilms |
| title_fullStr | MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> Biofilms |
| title_full_unstemmed | MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> Biofilms |
| title_short | MALDI Mass Spectrometry Imaging Reveals the Existence of an <i>N</i>-Acyl-homoserine Lactone Quorum Sensing System in <i>Pseudomonas putida</i> Biofilms |
| title_sort | maldi mass spectrometry imaging reveals the existence of an i n i acyl homoserine lactone quorum sensing system in i pseudomonas putida i biofilms |
| topic | mass spectrometry imaging quorum sensing <i>Pseudomonas putida</i> biofilms sample preparations |
| url | https://www.mdpi.com/2218-1989/12/11/1148 |
| work_keys_str_mv | AT rattanaburipitchapa maldimassspectrometryimagingrevealstheexistenceofaniniacylhomoserinelactonequorumsensingsysteminipseudomonasputidaibiofilms AT sivamokedissook maldimassspectrometryimagingrevealstheexistenceofaniniacylhomoserinelactonequorumsensingsysteminipseudomonasputidaibiofilms AT sastiapramaputri maldimassspectrometryimagingrevealstheexistenceofaniniacylhomoserinelactonequorumsensingsysteminipseudomonasputidaibiofilms AT eiichirofukusaki maldimassspectrometryimagingrevealstheexistenceofaniniacylhomoserinelactonequorumsensingsysteminipseudomonasputidaibiofilms AT shuichishimma maldimassspectrometryimagingrevealstheexistenceofaniniacylhomoserinelactonequorumsensingsysteminipseudomonasputidaibiofilms |