<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35
<i>Mycoplasma genitalium</i> is a newly recognized pathogen associated with sexually transmitted diseases (STDs). MgPa, the adhesion protein of <i>Mycoplasma genitalium</i>, is the main adhesin and the key factor for <i>M. genitalium</i> interacting with host cell...
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MDPI AG
2021-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/11/1449 |
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| author | Pei Dai Xiangying Deng Peng Liu Lingling Li Dan Luo Yating Liao Yanhua Zeng |
| author_facet | Pei Dai Xiangying Deng Peng Liu Lingling Li Dan Luo Yating Liao Yanhua Zeng |
| author_sort | Pei Dai |
| collection | DOAJ |
| description | <i>Mycoplasma genitalium</i> is a newly recognized pathogen associated with sexually transmitted diseases (STDs). MgPa, the adhesion protein of <i>Mycoplasma genitalium</i>, is the main adhesin and the key factor for <i>M. genitalium</i> interacting with host cells. Currently, the long-term survival mechanism of <i>M. genitalium</i> in the host is not clear. In this study, a T7 phage-displayed human urothelial cell (SV-HUC-1) cDNA library was constructed, and the interaction of MgPa was screened from this library using the recombinant MgPa (rMgPa) as a target molecule. We verified that 60S ribosomal protein L35 (RPL35) can interact with MgPa using far-Western blot and co-localization analysis. According to the results of tandem mass tag (TMT) labeling and proteome quantitative analysis, there were altogether 407 differentially expressed proteins between the pcDNA3.1(+)/MgPa-transfected cells and non-transfected cells, of which there were 6 downregulated proteins and 401 upregulated proteins. The results of qRT-PCR demonstrated that interaction between rMgPa and RPL35 could promote the expressions of EIF2, SRP68, SERBP1, RPL35A, EGF, and TGF-β. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide bromide (MTT) assays corroborated that the interaction between rMgPa and RPL35 could promote SV-HUC-1 cell proliferation. Therefore, our findings indicated that the interaction between rMgPa and RPL35 can enhance the expressions of transcription-initiation and translation-related proteins and thus promote cell proliferation. This study elucidates a new biological function of MgPa and can explain this new mechanism of <i>M. genitalium</i> in the host. |
| first_indexed | 2024-03-10T05:09:34Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T05:09:34Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-04fced3f4cb44b2d96dc71a1a45157cc2023-11-23T00:53:29ZengMDPI AGPathogens2076-08172021-11-011011144910.3390/pathogens10111449<i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35Pei Dai0Xiangying Deng1Peng Liu2Lingling Li3Dan Luo4Yating Liao5Yanhua Zeng6Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, ChinaHunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, ChinaHunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, ChinaHunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, ChinaHunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, ChinaHunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, ChinaHunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, China<i>Mycoplasma genitalium</i> is a newly recognized pathogen associated with sexually transmitted diseases (STDs). MgPa, the adhesion protein of <i>Mycoplasma genitalium</i>, is the main adhesin and the key factor for <i>M. genitalium</i> interacting with host cells. Currently, the long-term survival mechanism of <i>M. genitalium</i> in the host is not clear. In this study, a T7 phage-displayed human urothelial cell (SV-HUC-1) cDNA library was constructed, and the interaction of MgPa was screened from this library using the recombinant MgPa (rMgPa) as a target molecule. We verified that 60S ribosomal protein L35 (RPL35) can interact with MgPa using far-Western blot and co-localization analysis. According to the results of tandem mass tag (TMT) labeling and proteome quantitative analysis, there were altogether 407 differentially expressed proteins between the pcDNA3.1(+)/MgPa-transfected cells and non-transfected cells, of which there were 6 downregulated proteins and 401 upregulated proteins. The results of qRT-PCR demonstrated that interaction between rMgPa and RPL35 could promote the expressions of EIF2, SRP68, SERBP1, RPL35A, EGF, and TGF-β. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide bromide (MTT) assays corroborated that the interaction between rMgPa and RPL35 could promote SV-HUC-1 cell proliferation. Therefore, our findings indicated that the interaction between rMgPa and RPL35 can enhance the expressions of transcription-initiation and translation-related proteins and thus promote cell proliferation. This study elucidates a new biological function of MgPa and can explain this new mechanism of <i>M. genitalium</i> in the host.https://www.mdpi.com/2076-0817/10/11/1449<i>Mycoplasma genitalium</i>MgPaT7 phage-displayed cDNA libraryinteracting proteinsRPL35 |
| spellingShingle | Pei Dai Xiangying Deng Peng Liu Lingling Li Dan Luo Yating Liao Yanhua Zeng <i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35 Pathogens <i>Mycoplasma genitalium</i> MgPa T7 phage-displayed cDNA library interacting proteins RPL35 |
| title | <i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35 |
| title_full | <i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35 |
| title_fullStr | <i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35 |
| title_full_unstemmed | <i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35 |
| title_short | <i>Mycoplasma genitalium</i> Protein of Adhesion Promotes the Early Proliferation of Human Urothelial Cells by Interacting with RPL35 |
| title_sort | i mycoplasma genitalium i protein of adhesion promotes the early proliferation of human urothelial cells by interacting with rpl35 |
| topic | <i>Mycoplasma genitalium</i> MgPa T7 phage-displayed cDNA library interacting proteins RPL35 |
| url | https://www.mdpi.com/2076-0817/10/11/1449 |
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