Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathway
Abstract Introduction Previous evidence realized the critical role of histone in disease control. The anti‐inflammatory function of estradiol (E2) in sepsis has been documented. We here intended to unveil the role of extracellular histone H3 in sepsis regarding cell ferroptosis and the role of E2 in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.754 |
| _version_ | 1811176805794578432 |
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| author | Zhijun Han Zhizhou Yuan Linfei Shu Tao Li Fan Yang Lei Chen |
| author_facet | Zhijun Han Zhizhou Yuan Linfei Shu Tao Li Fan Yang Lei Chen |
| author_sort | Zhijun Han |
| collection | DOAJ |
| description | Abstract Introduction Previous evidence realized the critical role of histone in disease control. The anti‐inflammatory function of estradiol (E2) in sepsis has been documented. We here intended to unveil the role of extracellular histone H3 in sepsis regarding cell ferroptosis and the role of E2 in a such mechanism. Methods Clinical sample, cecal ligation and puncture (CLP)‐induced animal models and lipopolysaccharides (LPS)‐induced cell models were prepared for testing relative expression of extracellular histone H3 and E2 as well as analyzing the role of extracellular histone H3 and E2 in sepsis concerning cell viability, reactive oxygen species (ROS), and ferroptosis. Results Under sepsis, we found increased ferroptosis and extracellular histone H3 content, but reduced E2 concentration. Extracellular histone H3 facilitated ferroptosis of human umbilical vein endothelial cells (HUVECs) induced by LPS through activating the ROS/c‐Jun N‐terminal kinase (JNK) pathway. Moreover, E2 antagonized the effect of extracellular histone H3 on LPS‐induced HUVEC ferroptosis and sepsis injury in CLP‐induced animal models. Conclusion We highlighted that extracellular histone H3 facilitated lipopolysaccharides‐induced HUVEC ferroptosis via activating ROS/JNK pathway, and such an effect could be antagonized by E2. |
| first_indexed | 2024-04-10T19:57:46Z |
| format | Article |
| id | doaj.art-04ff6c57118e44dca1f8eebbfadb8405 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-04-10T19:57:46Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-04ff6c57118e44dca1f8eebbfadb84052023-01-27T13:18:32ZengWileyImmunity, Inflammation and Disease2050-45272023-01-01111n/an/a10.1002/iid3.754Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathwayZhijun Han0Zhizhou Yuan1Linfei Shu2Tao Li3Fan Yang4Lei Chen5Department of Urology Surgery Zhuzhou Central Hospital Zhuzhou Hunan Province ChinaDepartment of Urology Surgery Zhuzhou Central Hospital Zhuzhou Hunan Province ChinaDepartment of Urology Surgery Zhuzhou Central Hospital Zhuzhou Hunan Province ChinaThe Second Affiliated Hospital of Hainan Medical University Haikou Hainan Province ChinaDepartment of Urology Surgery Zhuzhou Central Hospital Zhuzhou Hunan Province ChinaDepartment of Urology Surgery Zhuzhou Central Hospital Zhuzhou Hunan Province ChinaAbstract Introduction Previous evidence realized the critical role of histone in disease control. The anti‐inflammatory function of estradiol (E2) in sepsis has been documented. We here intended to unveil the role of extracellular histone H3 in sepsis regarding cell ferroptosis and the role of E2 in a such mechanism. Methods Clinical sample, cecal ligation and puncture (CLP)‐induced animal models and lipopolysaccharides (LPS)‐induced cell models were prepared for testing relative expression of extracellular histone H3 and E2 as well as analyzing the role of extracellular histone H3 and E2 in sepsis concerning cell viability, reactive oxygen species (ROS), and ferroptosis. Results Under sepsis, we found increased ferroptosis and extracellular histone H3 content, but reduced E2 concentration. Extracellular histone H3 facilitated ferroptosis of human umbilical vein endothelial cells (HUVECs) induced by LPS through activating the ROS/c‐Jun N‐terminal kinase (JNK) pathway. Moreover, E2 antagonized the effect of extracellular histone H3 on LPS‐induced HUVEC ferroptosis and sepsis injury in CLP‐induced animal models. Conclusion We highlighted that extracellular histone H3 facilitated lipopolysaccharides‐induced HUVEC ferroptosis via activating ROS/JNK pathway, and such an effect could be antagonized by E2.https://doi.org/10.1002/iid3.754c‐Jun N‐terminal kinaseestradiolferroptosishistones H3reactive oxygen speciessepsis |
| spellingShingle | Zhijun Han Zhizhou Yuan Linfei Shu Tao Li Fan Yang Lei Chen Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathway Immunity, Inflammation and Disease c‐Jun N‐terminal kinase estradiol ferroptosis histones H3 reactive oxygen species sepsis |
| title | Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathway |
| title_full | Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathway |
| title_fullStr | Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathway |
| title_full_unstemmed | Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathway |
| title_short | Extracellular histone H3 facilitates ferroptosis in sepsis through ROS/JNK pathway |
| title_sort | extracellular histone h3 facilitates ferroptosis in sepsis through ros jnk pathway |
| topic | c‐Jun N‐terminal kinase estradiol ferroptosis histones H3 reactive oxygen species sepsis |
| url | https://doi.org/10.1002/iid3.754 |
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