Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant
Anthocyanin is abundant in a few vegetables, including eggplant. It protects plants from abiotic stress and benefits human health, making the research of anthocyanin biosynthesis increasingly important. Flowering time is an important reference for judging reproduction and adaptability, which can gui...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-01-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014120300868 |
| _version_ | 1797203273148530688 |
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| author | Haochun Xi Yongjun He Huoying Chen |
| author_facet | Haochun Xi Yongjun He Huoying Chen |
| author_sort | Haochun Xi |
| collection | DOAJ |
| description | Anthocyanin is abundant in a few vegetables, including eggplant. It protects plants from abiotic stress and benefits human health, making the research of anthocyanin biosynthesis increasingly important. Flowering time is an important reference for judging reproduction and adaptability, which can guide plant production. In this study, SmbHLH13 from eggplant was identified. Yeast one-hybrid and dual-luciferase assays showed that SmbHLH13 binded and activated the expression of structural genes SmCHS and SmF3H in anthocyanin biosynthesis and it also was bound to the promoter of the key gene SmFT in flowering. Furthermore, genetic transformation of Arabidopsis revealed that overexpression of SmbHLH13 enhanced anthocyanin accumulation and delayed flowering. These results demonstrated that SmbHLH13 might promote anthocyanin accumulation through positive regulation of SmCHS and SmF3H. Moreover, SmbHLH13 might have a role in delaying eggplant flowering. |
| first_indexed | 2024-04-24T08:16:42Z |
| format | Article |
| id | doaj.art-0501e9f834454e52886d0d7d1866637f |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:16:42Z |
| publishDate | 2021-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-0501e9f834454e52886d0d7d1866637f2024-04-17T03:05:15ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412021-01-01717380Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in EggplantHaochun Xi0Yongjun He1Huoying Chen2School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai JiaoTong University, Shanghai 200240, ChinaCorresponding author: Tel: +86 21 34206934.; School of Agriculture and Biology, Shanghai JiaoTong University, Shanghai 200240, ChinaAnthocyanin is abundant in a few vegetables, including eggplant. It protects plants from abiotic stress and benefits human health, making the research of anthocyanin biosynthesis increasingly important. Flowering time is an important reference for judging reproduction and adaptability, which can guide plant production. In this study, SmbHLH13 from eggplant was identified. Yeast one-hybrid and dual-luciferase assays showed that SmbHLH13 binded and activated the expression of structural genes SmCHS and SmF3H in anthocyanin biosynthesis and it also was bound to the promoter of the key gene SmFT in flowering. Furthermore, genetic transformation of Arabidopsis revealed that overexpression of SmbHLH13 enhanced anthocyanin accumulation and delayed flowering. These results demonstrated that SmbHLH13 might promote anthocyanin accumulation through positive regulation of SmCHS and SmF3H. Moreover, SmbHLH13 might have a role in delaying eggplant flowering.http://www.sciencedirect.com/science/article/pii/S2468014120300868eggplantSolanum melongenatranscription factoranthocyanin biosynthesisflowering |
| spellingShingle | Haochun Xi Yongjun He Huoying Chen Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant Horticultural Plant Journal eggplant Solanum melongena transcription factor anthocyanin biosynthesis flowering |
| title | Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant |
| title_full | Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant |
| title_fullStr | Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant |
| title_full_unstemmed | Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant |
| title_short | Functional Characterization of SmbHLH13 in Anthocyanin Biosynthesis and Flowering in Eggplant |
| title_sort | functional characterization of smbhlh13 in anthocyanin biosynthesis and flowering in eggplant |
| topic | eggplant Solanum melongena transcription factor anthocyanin biosynthesis flowering |
| url | http://www.sciencedirect.com/science/article/pii/S2468014120300868 |
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