Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

BACKGROUND: 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the out...

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Bibliographic Details
Main Authors: Hideyuki Tamaki, Chris L Wright, Xiangzhen Li, Qiaoyan Lin, Chiachi Hwang, Shiping Wang, Jyothi Thimmapuram, Yoichi Kamagata, Wen-Tso Liu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3179495?pdf=render

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