Expression levels of filaggrin-2 in relation to drip loss in pigs
Objective The aim of this study was to investigate the expression level of filaggrin-2 (FLG2) in correlation with drip loss. Methods The muscle samples were randomly taken from a local meat supplier. Samples were taken from Longissimus lumborum muscles to evaluate the drip loss (n = 100). Five muscl...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Asian-Australasian Association of Animal Production Societies
2022-04-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-21-0220.pdf |
| _version_ | 1830510877963452416 |
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| author | Autchara Kayan Nunyarat Koomkrong |
| author_facet | Autchara Kayan Nunyarat Koomkrong |
| author_sort | Autchara Kayan |
| collection | DOAJ |
| description | Objective The aim of this study was to investigate the expression level of filaggrin-2 (FLG2) in correlation with drip loss. Methods The muscle samples were randomly taken from a local meat supplier. Samples were taken from Longissimus lumborum muscles to evaluate the drip loss (n = 100). Five muscles per group (low and high drip loss) were selected to evaluate FLG2 mRNA and protein expression levels. Results mRNA of FLG2 gene was not significantly different in pigs with different levels of drip loss (p>0.05). Statistical analysis revealed that FLG2 protein expression levels were significantly different between the drip loss groups. Western blot revealed that the high drip loss group had higher FLG2 protein expression level than the low drip loss group (p<0.001). Moreover, immunohistochemistry revealed the high signal intensity was on the muscle cell membrane and cytoplasm. Conclusion FLG2 protein might play roles in drip loss of pork and will provide the basis for information to improving meat quality traits in pigs. |
| first_indexed | 2024-12-22T02:00:36Z |
| format | Article |
| id | doaj.art-050491224e6543afa78b901b998e945f |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-12-22T02:00:36Z |
| publishDate | 2022-04-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-050491224e6543afa78b901b998e945f2022-12-21T18:42:39ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352022-04-0135462463010.5713/ab.21.022024777Expression levels of filaggrin-2 in relation to drip loss in pigsAutchara Kayan0Nunyarat Koomkrong1 Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand Department of Animal Science, Faculty of Science and Technology, Suratthani Rajabhat University, Suratthani 84100, ThailandObjective The aim of this study was to investigate the expression level of filaggrin-2 (FLG2) in correlation with drip loss. Methods The muscle samples were randomly taken from a local meat supplier. Samples were taken from Longissimus lumborum muscles to evaluate the drip loss (n = 100). Five muscles per group (low and high drip loss) were selected to evaluate FLG2 mRNA and protein expression levels. Results mRNA of FLG2 gene was not significantly different in pigs with different levels of drip loss (p>0.05). Statistical analysis revealed that FLG2 protein expression levels were significantly different between the drip loss groups. Western blot revealed that the high drip loss group had higher FLG2 protein expression level than the low drip loss group (p<0.001). Moreover, immunohistochemistry revealed the high signal intensity was on the muscle cell membrane and cytoplasm. Conclusion FLG2 protein might play roles in drip loss of pork and will provide the basis for information to improving meat quality traits in pigs.http://www.animbiosci.org/upload/pdf/ab-21-0220.pdfdrip lossexpressionfilaggrin-2 (flg2)pig |
| spellingShingle | Autchara Kayan Nunyarat Koomkrong Expression levels of filaggrin-2 in relation to drip loss in pigs Animal Bioscience drip loss expression filaggrin-2 (flg2) pig |
| title | Expression levels of filaggrin-2 in relation to drip loss in pigs |
| title_full | Expression levels of filaggrin-2 in relation to drip loss in pigs |
| title_fullStr | Expression levels of filaggrin-2 in relation to drip loss in pigs |
| title_full_unstemmed | Expression levels of filaggrin-2 in relation to drip loss in pigs |
| title_short | Expression levels of filaggrin-2 in relation to drip loss in pigs |
| title_sort | expression levels of filaggrin 2 in relation to drip loss in pigs |
| topic | drip loss expression filaggrin-2 (flg2) pig |
| url | http://www.animbiosci.org/upload/pdf/ab-21-0220.pdf |
| work_keys_str_mv | AT autcharakayan expressionlevelsoffilaggrin2inrelationtodriplossinpigs AT nunyaratkoomkrong expressionlevelsoffilaggrin2inrelationtodriplossinpigs |