Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes
Abstract Aims/Introduction Genetic and epigenetic mechanisms have been implicated in the pathogenesis of type 1 diabetes, and histone acetylation is an epigenetic modification pattern that activates gene transcription. However, the genome‐wide histone H3 acetylation in new‐onset type 1 diabetes pati...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Diabetes Investigation |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jdi.12867 |
| _version_ | 1818600127288311808 |
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| author | Yanfei Wang Can Hou Jonathan Wisler Kanhaiya Singh Chao Wu Zhiguo Xie Qianjin Lu Zhiguang Zhou |
| author_facet | Yanfei Wang Can Hou Jonathan Wisler Kanhaiya Singh Chao Wu Zhiguo Xie Qianjin Lu Zhiguang Zhou |
| author_sort | Yanfei Wang |
| collection | DOAJ |
| description | Abstract Aims/Introduction Genetic and epigenetic mechanisms have been implicated in the pathogenesis of type 1 diabetes, and histone acetylation is an epigenetic modification pattern that activates gene transcription. However, the genome‐wide histone H3 acetylation in new‐onset type 1 diabetes patients has not been well described. Accordingly, we aimed to unveil the genome‐wide promoter acetylation profile in CD4+ T lymphocytes from type 1 diabetes patients, especially for those who are glutamate decarboxylase antibody‐positive. Materials and Methods A total of 12 patients with new‐onset type 1 diabetes who were glutamate decarboxylase antibody‐positive were enrolled, and 12 healthy individuals were recruited as controls. The global histone H3 acetylation level of CD4+ T lymphocytes from peripheral blood was detected by western blot, with chromatin immunoprecipitation linked to microarrays to characterize the promoter acetylation profile. Furthermore, we validated the results of particular genes from chromatin immunoprecipitation linked to microarrays by using chromatin immunoprecipitation quantitative polymerase chain reaction, and analyzed the transcription level by real‐time quantitative polymerase chain reaction. Results Elevated global histone H3 acetylation level was observed in type 1 diabetes patients, with 607 differentially acetylated genes identified between type 1 diabetes patients and controls by chromatin immunoprecipitation linked to microarrays. The hyperacetylated genes were enriched in biological processes involved in immune cell activation and inflammatory response. Gene‐specific assessments showed that increased transcription of inducible T‐cell costimulator was in concordance with the elevated acetylation in its gene promoter, along with positive correlation with glutamate decarboxylase antibody titer in type 1 diabetes patients. Conclusions The present study generates a genome‐wide histone acetylation profile specific to CD4+ T lymphocytes in type 1 diabetes patients who are glutamic acid decarboxylase antibody‐positive, which is instrumental in improving our understanding of the epigenetic involvement in autoimmune diabetes. |
| first_indexed | 2024-12-16T12:30:33Z |
| format | Article |
| id | doaj.art-05076a5b0d114f9381f6af9760b437d4 |
| institution | Directory Open Access Journal |
| issn | 2040-1116 2040-1124 |
| language | English |
| last_indexed | 2024-12-16T12:30:33Z |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Investigation |
| spelling | doaj.art-05076a5b0d114f9381f6af9760b437d42022-12-21T22:31:43ZengWileyJournal of Diabetes Investigation2040-11162040-11242019-01-01101516110.1111/jdi.12867Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetesYanfei Wang0Can Hou1Jonathan Wisler2Kanhaiya Singh3Chao Wu4Zhiguo Xie5Qianjin Lu6Zhiguang Zhou7Department of Metabolism & Endocrinology The Second Xiangya Hospital Central South UniversityChangsha Hunan ChinaDepartment of Intensive Care Unit The Second Xiangya Hospital Central South University Changsha Hunan ChinaDepartment of Surgery Division of Trauma, Critical Care and Burn SurgeryThe Ohio State University Wexner Medical Center Columbus Ohio USADepartment of Surgery The Ohio State University Wexner Medical Center Columbus Ohio USADepartment of Metabolism & Endocrinology The Second Xiangya Hospital Central South UniversityChangsha Hunan ChinaDepartment of Metabolism & Endocrinology The Second Xiangya Hospital Central South UniversityChangsha Hunan ChinaDepartment of Dermatology The Second Xiangya Hospital Central South University Changsha Hunan ChinaDepartment of Metabolism & Endocrinology The Second Xiangya Hospital Central South UniversityChangsha Hunan ChinaAbstract Aims/Introduction Genetic and epigenetic mechanisms have been implicated in the pathogenesis of type 1 diabetes, and histone acetylation is an epigenetic modification pattern that activates gene transcription. However, the genome‐wide histone H3 acetylation in new‐onset type 1 diabetes patients has not been well described. Accordingly, we aimed to unveil the genome‐wide promoter acetylation profile in CD4+ T lymphocytes from type 1 diabetes patients, especially for those who are glutamate decarboxylase antibody‐positive. Materials and Methods A total of 12 patients with new‐onset type 1 diabetes who were glutamate decarboxylase antibody‐positive were enrolled, and 12 healthy individuals were recruited as controls. The global histone H3 acetylation level of CD4+ T lymphocytes from peripheral blood was detected by western blot, with chromatin immunoprecipitation linked to microarrays to characterize the promoter acetylation profile. Furthermore, we validated the results of particular genes from chromatin immunoprecipitation linked to microarrays by using chromatin immunoprecipitation quantitative polymerase chain reaction, and analyzed the transcription level by real‐time quantitative polymerase chain reaction. Results Elevated global histone H3 acetylation level was observed in type 1 diabetes patients, with 607 differentially acetylated genes identified between type 1 diabetes patients and controls by chromatin immunoprecipitation linked to microarrays. The hyperacetylated genes were enriched in biological processes involved in immune cell activation and inflammatory response. Gene‐specific assessments showed that increased transcription of inducible T‐cell costimulator was in concordance with the elevated acetylation in its gene promoter, along with positive correlation with glutamate decarboxylase antibody titer in type 1 diabetes patients. Conclusions The present study generates a genome‐wide histone acetylation profile specific to CD4+ T lymphocytes in type 1 diabetes patients who are glutamic acid decarboxylase antibody‐positive, which is instrumental in improving our understanding of the epigenetic involvement in autoimmune diabetes.https://doi.org/10.1111/jdi.12867CD4+ T lymphocytesHistone H3 acetylation profileType 1 diabetes |
| spellingShingle | Yanfei Wang Can Hou Jonathan Wisler Kanhaiya Singh Chao Wu Zhiguo Xie Qianjin Lu Zhiguang Zhou Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes Journal of Diabetes Investigation CD4+ T lymphocytes Histone H3 acetylation profile Type 1 diabetes |
| title | Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes |
| title_full | Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes |
| title_fullStr | Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes |
| title_full_unstemmed | Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes |
| title_short | Elevated histone H3 acetylation is associated with genes involved in T lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes |
| title_sort | elevated histone h3 acetylation is associated with genes involved in t lymphocyte activation and glutamate decarboxylase antibody production in patients with type 1 diabetes |
| topic | CD4+ T lymphocytes Histone H3 acetylation profile Type 1 diabetes |
| url | https://doi.org/10.1111/jdi.12867 |
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