Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection
The Toll-like receptors (TLR), being pattern recognition molecules, are a powerful first line of defense in response to pathogen invasion. They are known to play a crucial role in detecting and binding to the microbial molecule and triggering a non-specific immune response. Quantitative real time PC...
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| Format: | Article |
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Elsevier
2015-05-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513415000071 |
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| author | Mundanda Muthappa Dechamma Moger Rajeish Biswajit Maiti Madhu K. Mani Indrani Karunasagar |
| author_facet | Mundanda Muthappa Dechamma Moger Rajeish Biswajit Maiti Madhu K. Mani Indrani Karunasagar |
| author_sort | Mundanda Muthappa Dechamma |
| collection | DOAJ |
| description | The Toll-like receptors (TLR), being pattern recognition molecules, are a powerful first line of defense in response to pathogen invasion. They are known to play a crucial role in detecting and binding to the microbial molecule and triggering a non-specific immune response. Quantitative real time PCR (qPCR) expression of the TLR gene was studied in healthy and Vibrio harveyi infected black tiger shrimp (Penaeus monodon). Lymphoid tissue expression of TLR in V. harveyi infected animals 24 h post injection showed statistically significant up regulation of the gene as compared to the control animals sham injected with phosphate buffered saline (PBS). The qPCR expression pattern of TLR at different time points in shrimp administered with the immunostimulant glucan for 6 days by oral feeding followed by challenge with V. harveyi showed statistically significant level at 48 h post bacterial challenge as compared to the control (immunostimulant treated) animals sham injected with PBS. The novelty of the study is that it elicits the role of TLRs as important response proteins of the innate immune system in the shrimp. |
| first_indexed | 2024-04-13T18:40:22Z |
| format | Article |
| id | doaj.art-05081390f42e4a78bcc5446834863859 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-13T18:40:22Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-05081390f42e4a78bcc54468348638592022-12-22T02:34:44ZengElsevierAquaculture Reports2352-51342015-05-011C1410.1016/j.aqrep.2015.02.002Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infectionMundanda Muthappa Dechamma0Moger Rajeish1Biswajit Maiti2Madhu K. Mani3Indrani Karunasagar4Department of Fisheries Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575002, IndiaDepartment of Fisheries Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575002, IndiaFaculty of Biomedical Sciences, UNESCO–MIRCEN for Medical & Marine Biotechnology, Nitte University Center for Science Education and Research, Nitte University, Deralakatte, Mangalore 575018, IndiaDepartment of Fisheries Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575002, IndiaFaculty of Biomedical Sciences, UNESCO–MIRCEN for Medical & Marine Biotechnology, Nitte University Center for Science Education and Research, Nitte University, Deralakatte, Mangalore 575018, IndiaThe Toll-like receptors (TLR), being pattern recognition molecules, are a powerful first line of defense in response to pathogen invasion. They are known to play a crucial role in detecting and binding to the microbial molecule and triggering a non-specific immune response. Quantitative real time PCR (qPCR) expression of the TLR gene was studied in healthy and Vibrio harveyi infected black tiger shrimp (Penaeus monodon). Lymphoid tissue expression of TLR in V. harveyi infected animals 24 h post injection showed statistically significant up regulation of the gene as compared to the control animals sham injected with phosphate buffered saline (PBS). The qPCR expression pattern of TLR at different time points in shrimp administered with the immunostimulant glucan for 6 days by oral feeding followed by challenge with V. harveyi showed statistically significant level at 48 h post bacterial challenge as compared to the control (immunostimulant treated) animals sham injected with PBS. The novelty of the study is that it elicits the role of TLRs as important response proteins of the innate immune system in the shrimp.http://www.sciencedirect.com/science/article/pii/S2352513415000071Toll-like receptorsPenaeus monodonVibrio harveyiReal time PCRImmunostimulant |
| spellingShingle | Mundanda Muthappa Dechamma Moger Rajeish Biswajit Maiti Madhu K. Mani Indrani Karunasagar Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection Aquaculture Reports Toll-like receptors Penaeus monodon Vibrio harveyi Real time PCR Immunostimulant |
| title | Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection |
| title_full | Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection |
| title_fullStr | Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection |
| title_full_unstemmed | Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection |
| title_short | Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection |
| title_sort | expression of toll like receptors tlr in lymphoid organ of black tiger shrimp penaeus monodon in response to vibrio harveyi infection |
| topic | Toll-like receptors Penaeus monodon Vibrio harveyi Real time PCR Immunostimulant |
| url | http://www.sciencedirect.com/science/article/pii/S2352513415000071 |
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