Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior
Synthetic peptides are promising structural and functional components of bioactive and tissue-engineering scaffolds. Here, we demonstrate the design of self-assembling nanofiber scaffolds based on peptide amphiphile (PA) molecules containing multi-functional histidine residues with trace metal (TM)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/4/883 |
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| author | Kenana Dayob Aygul Zengin Ruslan Garifullin Mustafa O. Guler Timur I. Abdullin Abdulla Yergeshov Diana V. Salakhieva Hong Hanh Cong Mohamed Zoughaib |
| author_facet | Kenana Dayob Aygul Zengin Ruslan Garifullin Mustafa O. Guler Timur I. Abdullin Abdulla Yergeshov Diana V. Salakhieva Hong Hanh Cong Mohamed Zoughaib |
| author_sort | Kenana Dayob |
| collection | DOAJ |
| description | Synthetic peptides are promising structural and functional components of bioactive and tissue-engineering scaffolds. Here, we demonstrate the design of self-assembling nanofiber scaffolds based on peptide amphiphile (PA) molecules containing multi-functional histidine residues with trace metal (TM) coordination ability. The self-assembly of PAs and characteristics of PA nanofiber scaffolds along with their interaction with Zn, Cu, and Mn essential microelements were studied. The effects of TM-activated PA scaffolds on mammalian cell behavior, reactive oxygen species (ROS), and glutathione levels were shown. The study reveals the ability of these scaffolds to modulate adhesion, proliferation, and morphological differentiation of neuronal PC-12 cells, suggesting a particular role of Mn(II) in cell-matrix interaction and neuritogenesis. The results provide a proof-of-concept for the development of histidine-functionalized peptide nanofiber scaffolds activated with ROS- and cell-modulating TMs to induce regenerative responses. |
| first_indexed | 2024-03-11T04:43:10Z |
| format | Article |
| id | doaj.art-05099fe4153a444d9be8b68981661b28 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T04:43:10Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-05099fe4153a444d9be8b68981661b282023-11-17T20:30:46ZengMDPI AGMicromachines2072-666X2023-04-0114488310.3390/mi14040883Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell BehaviorKenana Dayob0Aygul Zengin1Ruslan Garifullin2Mustafa O. Guler3Timur I. Abdullin4Abdulla Yergeshov5Diana V. Salakhieva6Hong Hanh Cong7Mohamed Zoughaib8Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaDepartment of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The NetherlandsInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaThe Pritzker School of Molecular Engineering, The University of Chicago, Chicago, IL 60637, USAInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaInstitute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Hanoi 100000, VietnamInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 18 Kremlyovskaya St., 420008 Kazan, RussiaSynthetic peptides are promising structural and functional components of bioactive and tissue-engineering scaffolds. Here, we demonstrate the design of self-assembling nanofiber scaffolds based on peptide amphiphile (PA) molecules containing multi-functional histidine residues with trace metal (TM) coordination ability. The self-assembly of PAs and characteristics of PA nanofiber scaffolds along with their interaction with Zn, Cu, and Mn essential microelements were studied. The effects of TM-activated PA scaffolds on mammalian cell behavior, reactive oxygen species (ROS), and glutathione levels were shown. The study reveals the ability of these scaffolds to modulate adhesion, proliferation, and morphological differentiation of neuronal PC-12 cells, suggesting a particular role of Mn(II) in cell-matrix interaction and neuritogenesis. The results provide a proof-of-concept for the development of histidine-functionalized peptide nanofiber scaffolds activated with ROS- and cell-modulating TMs to induce regenerative responses.https://www.mdpi.com/2072-666X/14/4/883peptide amphiphilesself-assemblynanofiber scaffoldhistidinetrace metalsreactive oxygen species |
| spellingShingle | Kenana Dayob Aygul Zengin Ruslan Garifullin Mustafa O. Guler Timur I. Abdullin Abdulla Yergeshov Diana V. Salakhieva Hong Hanh Cong Mohamed Zoughaib Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior Micromachines peptide amphiphiles self-assembly nanofiber scaffold histidine trace metals reactive oxygen species |
| title | Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior |
| title_full | Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior |
| title_fullStr | Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior |
| title_full_unstemmed | Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior |
| title_short | Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior |
| title_sort | metal chelating self assembling peptide nanofiber scaffolds for modulation of neuronal cell behavior |
| topic | peptide amphiphiles self-assembly nanofiber scaffold histidine trace metals reactive oxygen species |
| url | https://www.mdpi.com/2072-666X/14/4/883 |
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