Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean Basin
Chitin is a structural polysaccharide of the cell walls of fungi and exoskeletons of insects and crustaceans. In this study, chitin was extracted, for the first time in our knowledge, from the <i>Cicada</i> <i>orni</i> sloughs of the south-eastern French Mediterranean basin b...
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MDPI AG
2020-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/11/2543 |
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| author | Aurelia Poerio Chloé Petit Jean-Philippe Jehl Elmira Arab-Tehrany João F. Mano Franck Cleymand |
| author_facet | Aurelia Poerio Chloé Petit Jean-Philippe Jehl Elmira Arab-Tehrany João F. Mano Franck Cleymand |
| author_sort | Aurelia Poerio |
| collection | DOAJ |
| description | Chitin is a structural polysaccharide of the cell walls of fungi and exoskeletons of insects and crustaceans. In this study, chitin was extracted, for the first time in our knowledge, from the <i>Cicada</i> <i>orni</i> sloughs of the south-eastern French Mediterranean basin by treatment with 1 M HCl for demineralization, 1 M NaOH for deproteinization, and 1% NaClO for decolorization. The different steps of extraction were investigated by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Scanning Electron Microscopy (SEM). Results demonstrated that the extraction process was efficiently performed and that <i>Cicada</i> <i>orni</i> sloughs of the south-eastern French Mediterranean basin have a high content of chitin (42.8%) in the α-form with a high degree of acetylation of 96% ± 3.4%. These results make <i>Cicada</i> <i>orni</i> of the south-eastern French Mediterranean basin a new and promising source of chitin. Furthermore, we showed that each step of the extraction present specific characteristics (for example FTIR and XRD spectra and, consequently, distinct absorbance peaks and values of crystallinity as well as defined values of maximum degradation temperatures identifiable by TGA analysis) that could be used to verify the effectiveness of the treatments, and could be favorably compared with other natural chitin sources. |
| first_indexed | 2024-03-10T19:29:18Z |
| format | Article |
| id | doaj.art-050b84f506ab41269c80134f641d41ca |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:29:18Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-050b84f506ab41269c80134f641d41ca2023-11-20T02:13:41ZengMDPI AGMolecules1420-30492020-05-012511254310.3390/molecules25112543Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean BasinAurelia Poerio0Chloé Petit1Jean-Philippe Jehl2Elmira Arab-Tehrany3João F. Mano4Franck Cleymand5Jean Lamour Institute, University of Lorraine, UMR 7198 CNRS, 2 allée André Guinier-Campus Artem, BP 50840, F-54011 Nancy CEDEX, FranceJean Lamour Institute, University of Lorraine, UMR 7198 CNRS, 2 allée André Guinier-Campus Artem, BP 50840, F-54011 Nancy CEDEX, FranceJean Lamour Institute, University of Lorraine, UMR 7198 CNRS, 2 allée André Guinier-Campus Artem, BP 50840, F-54011 Nancy CEDEX, FranceLaboratoire Ingénierie des Biomolécules, University of Lorraine, TSA 40602, F-54518 Vandoeuvre-lès-Nancy, FranceJean Lamour Institute, University of Lorraine, UMR 7198 CNRS, 2 allée André Guinier-Campus Artem, BP 50840, F-54011 Nancy CEDEX, FranceJean Lamour Institute, University of Lorraine, UMR 7198 CNRS, 2 allée André Guinier-Campus Artem, BP 50840, F-54011 Nancy CEDEX, FranceChitin is a structural polysaccharide of the cell walls of fungi and exoskeletons of insects and crustaceans. In this study, chitin was extracted, for the first time in our knowledge, from the <i>Cicada</i> <i>orni</i> sloughs of the south-eastern French Mediterranean basin by treatment with 1 M HCl for demineralization, 1 M NaOH for deproteinization, and 1% NaClO for decolorization. The different steps of extraction were investigated by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Scanning Electron Microscopy (SEM). Results demonstrated that the extraction process was efficiently performed and that <i>Cicada</i> <i>orni</i> sloughs of the south-eastern French Mediterranean basin have a high content of chitin (42.8%) in the α-form with a high degree of acetylation of 96% ± 3.4%. These results make <i>Cicada</i> <i>orni</i> of the south-eastern French Mediterranean basin a new and promising source of chitin. Furthermore, we showed that each step of the extraction present specific characteristics (for example FTIR and XRD spectra and, consequently, distinct absorbance peaks and values of crystallinity as well as defined values of maximum degradation temperatures identifiable by TGA analysis) that could be used to verify the effectiveness of the treatments, and could be favorably compared with other natural chitin sources.https://www.mdpi.com/1420-3049/25/11/2543<i>Cicada</i> <i>orni</i> sloughschitinextractionphysicochemical characterization |
| spellingShingle | Aurelia Poerio Chloé Petit Jean-Philippe Jehl Elmira Arab-Tehrany João F. Mano Franck Cleymand Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean Basin Molecules <i>Cicada</i> <i>orni</i> sloughs chitin extraction physicochemical characterization |
| title | Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean Basin |
| title_full | Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean Basin |
| title_fullStr | Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean Basin |
| title_full_unstemmed | Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean Basin |
| title_short | Extraction and Physicochemical Characterization of Chitin from <i>Cicada</i> <i>orni</i> Sloughs of the South-Eastern French Mediterranean Basin |
| title_sort | extraction and physicochemical characterization of chitin from i cicada i i orni i sloughs of the south eastern french mediterranean basin |
| topic | <i>Cicada</i> <i>orni</i> sloughs chitin extraction physicochemical characterization |
| url | https://www.mdpi.com/1420-3049/25/11/2543 |
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