Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage
BACKGROUND AND OBJECTIVE: Limited resources for adult stem cells necessitate appropriate methods for isolation, proliferation and differentiation of these cells. Human third molar is an easily available source in this regard that usually remains unused. The aim of this study was to investigate the i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Babol University of Medical Sciences
2014-12-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
| Subjects: | |
| Online Access: | http://jbums.org/browse.php?a_code=A-10-1472-1&slc_lang=en&sid=1 |
| _version_ | 1818500667334983680 |
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| author | Maryam Raoof ali Mohammadi Kamal-abadi Mohammad Mehdi Yaghoobi razieh kooshki Mehdi Abbasnejad ali Derakhshani |
| author_facet | Maryam Raoof ali Mohammadi Kamal-abadi Mohammad Mehdi Yaghoobi razieh kooshki Mehdi Abbasnejad ali Derakhshani |
| author_sort | Maryam Raoof |
| collection | DOAJ |
| description | BACKGROUND AND OBJECTIVE: Limited resources for adult
stem cells necessitate appropriate methods for isolation, proliferation and
differentiation of these cells. Human third molar is an easily available source
in this regard that usually remains unused.
The aim of this study was to investigate the isolation, culture and odontogenic
differentiation of human dental pulp stem cells.METHODS: In this experimental study, twenty human third molars were
used for stem cell isolation. The digested pulp tissue culture approach was
used to isolate stem cells. Some markers of stem cells were studied by RT-PCR.
Stem cells were then induced towards odontoblast cells by use of BMP-2 and
Dexamethasone. Expression of odontoblastic markers was investigated in induced
cells by RT-PCR and immunocytochemistry methods.FINDINGS: The human dental pulp stem cells were
isolated with high efficiency. RT-PCR
analysis showed that these cells express some markers of embryonic stem cells.
However, the induced cells did not expressed odontoblastic markers such as
DSPP, DMP-1, BMP7 and Osteopontin.
Also the induced cells did not produce DSPP and DMP-1 proteins.CONCLUSION: Despite the successful isolation of stem cells, the induced
cells failed to differentiate into odontoblasts. This may be due to the
differentiation method as well as the passage during induction or monolayer
culture. |
| first_indexed | 2024-12-10T20:45:50Z |
| format | Article |
| id | doaj.art-050ebf6d9a1641529c172ea0c933493a |
| institution | Directory Open Access Journal |
| issn | 1561-4107 2251-7170 |
| language | English |
| last_indexed | 2024-12-10T20:45:50Z |
| publishDate | 2014-12-01 |
| publisher | Babol University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
| spelling | doaj.art-050ebf6d9a1641529c172ea0c933493a2022-12-22T01:34:14ZengBabol University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul1561-41072251-71702014-12-0116122331Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic LineageMaryam Raoof0ali Mohammadi Kamal-abadi1Mohammad Mehdi Yaghoobi2razieh kooshki3Mehdi Abbasnejad4ali Derakhshani5 Department of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran. Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman,Iran. Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman,Iran. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran. Department of Pathology, Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran. BACKGROUND AND OBJECTIVE: Limited resources for adult stem cells necessitate appropriate methods for isolation, proliferation and differentiation of these cells. Human third molar is an easily available source in this regard that usually remains unused. The aim of this study was to investigate the isolation, culture and odontogenic differentiation of human dental pulp stem cells.METHODS: In this experimental study, twenty human third molars were used for stem cell isolation. The digested pulp tissue culture approach was used to isolate stem cells. Some markers of stem cells were studied by RT-PCR. Stem cells were then induced towards odontoblast cells by use of BMP-2 and Dexamethasone. Expression of odontoblastic markers was investigated in induced cells by RT-PCR and immunocytochemistry methods.FINDINGS: The human dental pulp stem cells were isolated with high efficiency. RT-PCR analysis showed that these cells express some markers of embryonic stem cells. However, the induced cells did not expressed odontoblastic markers such as DSPP, DMP-1, BMP7 and Osteopontin. Also the induced cells did not produce DSPP and DMP-1 proteins.CONCLUSION: Despite the successful isolation of stem cells, the induced cells failed to differentiate into odontoblasts. This may be due to the differentiation method as well as the passage during induction or monolayer culture.http://jbums.org/browse.php?a_code=A-10-1472-1&slc_lang=en&sid=1KEY WORDS: Dental Pulp Stem CellsOdontoblastsBMP-2Dexamethasone. |
| spellingShingle | Maryam Raoof ali Mohammadi Kamal-abadi Mohammad Mehdi Yaghoobi razieh kooshki Mehdi Abbasnejad ali Derakhshani Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul KEY WORDS: Dental Pulp Stem Cells Odontoblasts BMP-2 Dexamethasone. |
| title | Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage |
| title_full | Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage |
| title_fullStr | Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage |
| title_full_unstemmed | Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage |
| title_short | Isolation and Differentiation of Dental Pulp Stem Cells into Odontoblastic Lineage |
| title_sort | isolation and differentiation of dental pulp stem cells into odontoblastic lineage |
| topic | KEY WORDS: Dental Pulp Stem Cells Odontoblasts BMP-2 Dexamethasone. |
| url | http://jbums.org/browse.php?a_code=A-10-1472-1&slc_lang=en&sid=1 |
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